Intermittent swim stress effects on anxiety behavior

Millions of Americans are suffering from depression each year, leading to a significant number of individuals who seek treatment for their ailment. However, fewer than 50 percent of depressed individuals fully recover using current methods. The comorbidity between depression and anxiety could be a contributing factor in the lower rates of recovery. The demonstrated correlation between anxiety and depression has led to the term anxious depression, which is associated with difficulty in coping, a poorer rate of recovery, and more severe symptoms of depression. The purpose of this dissertation was to expand on an existing animal model of depression (intermittent swim stress) and its possible relationship to anxiety. In the intermittent swim stress (ISS) model, animals experienced 100, 5 second trials of cold water swim stress, and subsequent behavioral and cellular mechanisms were assessed. Behavioral measures incorporated animal models of anxiety (i.e., open field test and juvenile social exploration), while serotonergic and noradrenergic neurons were assessed at the dorsal raphe nucleus and locus coeruleus, respectively, through immunohistochemistry techniques. Results indicated ISS-induced deficits were noted for social exploration, but not with the open field test. No apparent cellular differences were revealed following the open field test, but this has yet to be investigated for juvenile social exploration. The anxiety effects produced by ISS support the trans-situational value of the model and also suggest ISS as a possible animal model of post-traumatic stress disorder. Future directions should assess cellular mechanisms following exposure to juvenile social exploration as well as explore the time course of the neural activity marker described in the experiments

Test order effects in simultaneous protocols

Simultaneous protocols typically yield poorer stimulus equivalence outcomes than do other protocols commonly used in equivalence research. Two independent groups of three 3-member equivalence sets of stimuli were used in conditional discrimination procedures in two conditions, one using the standard simultaneous protocol and the other using a hybrid simultaneous training and simple-to-complex testing. Participants completed the two conditions in one long session in Experiment 1, but in separate sessions in Experiment 2. The same stimulus sets used in Experiment 1 were randomized for the two conditions in Experiment 2. Overall, accuracy was better with the hybrid than with the standard protocol in both experiments. The equivalence yield was also better under the hybrid than under the standard protocol in each experiment. The results suggest that the order of testing for emergent relations may account for the difficulty often encountered with the standard simultaneous protocol

Change Detection Accuracy and Image Properties: A Study Using Simulated Data

Simulated data were used to investigate the relationships between image properties and change detection accuracy in a systematic manner. The image properties examined were class separability, radiometric normalization and image spectral band-to-band correlation. The change detection methods evaluated were post-classification comparison, direct classification of multidate imagery, image differencing, principal component analysis, and change vector analysis. The simulated data experiments showed that the relative accuracy of the change detection methods varied with changes in image properties, thus confirming the hypothesis that caution should be used in generalizing from studies that use only a single image pair. In most cases, direct classification and post-classification comparison were the least sensitive to changes in the image properties of class separability, radiometric normalization error and band correlation. Furthermore, these methods generally produced the highest accuracy, or were amongst those with a high accuracy. PCA accuracy was highly variable; the use of four principal components consistently resulted in substantial decreased classification accuracy relative to using six components, or classification using the original six bands. The accuracy of image differencing also varied greatly in the experiments. Of the three methods that require radiometric normalization, image differencing was the method most affected by radiometric error, relative to change vector and classification methods, for classes that have moderate and low separability. For classes that are highly separable, image differencing was relatively unaffected by radiometric normalization error. CVA was found to be the most accurate method for classes with low separability and all but the largest radiometric errors. CVA accuracy tended to be the least affected by changes in the degree of band correlation in situations where the class means were moderately dispersed, or clustered near the diagonal. For all change detection methods, the classification accuracy increased as simulated band correlation increased, and direct classification methods consistently had the highest accuracy, while PCA generally had the lowest accuracy

Evaluation of Sampling and Cross-Validation Tuning Strategies for Regional-Scale Machine Learning Classification

High spatial resolution (1–5 m) remotely sensed datasets are increasingly being used to map land covers over large geographic areas using supervised machine learning algorithms. Although many studies have compared machine learning classification methods, sample selection methods for acquiring training and validation data for machine learning, and cross-validation techniques for tuning classifier parameters are rarely investigated, particularly on large, high spatial resolution datasets. This work, therefore, examines four sample selection methods—simple random, proportional stratified random, disproportional stratified random, and deliberative sampling—as well as three cross-validation tuning approaches—k-fold, leave-one-out, and Monte Carlo methods. In addition, the effect on the accuracy of localizing sample selections to a small geographic subset of the entire area, an approach that is sometimes used to reduce costs associated with training data collection, is investigated. These methods are investigated in the context of support vector machines (SVM) classification and geographic object-based image analysis (GEOBIA), using high spatial resolution National Agricultural Imagery Program (NAIP) orthoimagery and LIDAR-derived rasters, covering a 2,609 km2 regional-scale area in northeastern West Virginia, USA. Stratified-statistical-based sampling methods were found to generate the highest classification accuracy. Using a small number of training samples collected from only a subset of the study area provided a similar level of overall accuracy to a sample of equivalent size collected in a dispersed manner across the entire regional-scale dataset. There were minimal differences in accuracy for the different cross-validation tuning methods. The processing time for Monte Carlo and leave-one-out cross-validation were high, especially with large training sets. For this reason, k-fold cross-validation appears to be a good choice. Classifications trained with samples collected deliberately (i.e., not randomly) were less accurate than classifiers trained from statistical-based samples. This may be due to the high positive spatial autocorrelation in the deliberative training set. Thus, if possible, samples for training should be selected randomly; deliberative samples should be avoided

Expression profiling of snoRNAs in normal hematopoiesis and AML

Key Points A subset of snoRNAs is expressed in a developmental- and lineage-specific manner during human hematopoiesis. Neither host gene expression nor alternative splicing accounted for the observed differential expression of snoRNAs in a subset of AML.</jats:p

Reducing Uncertainties in Applying Remotely Sensed Land Use and Land Cover Maps in Land-Atmosphere Interaction: Identifying Change in Space and Time

Land use and land cover (LULC) data are a central component of most land-atmosphere interaction studies, but there are two common and highly problematic scale mismatches between LULC and climate data. First, in the spatial domain, researchers rarely consider the impact of scaling up fine-scale LULC data to match coarse-scale climate datasets. Second, in the temporal domain, climate data typically have sub-daily, daily, monthly, or annual resolution, but LULC datasets often have much coarser (e.g., decadal) resolution. We first explored the effect of three spatial scaling methods on correlations among LULC data and a land surface climatic variable, latent heat flux in China. Scaling by a fractional method preserved significant correlations among LULC data and latent heat flux at all three studied scales (0.5°, 1.0°, and 2.5°), whereas nearest-neighbor and majority-aggregation methods caused these correlations to diminish and even become statistically non-significant at coarser spatial scales (i.e., 2.5°). In the temporal domain, we identified fractional changes in croplands, forests, and grasslands in China using a recently developed and annually resolved time series of LULC maps from 1982 to 2012. Relative to common LULC change (LULCC) analyses conducted over two-time steps or several time periods, this annually resolved, 31-year time series of LULC maps enables robust interpretation of LULCC. Specifically, the annual resolution of these data enabled us to more precisely observe three key and statistically significant LULCC trends and transitions that could have consequential effects on land-atmosphere interaction: (1) decreasing grasslands to increasing croplands in the Northeast China plain and the Yellow river basin, (2) decreasing croplands to increasing forests in the Yangtze river basin, and (3) decreasing grasslands to increasing forests in Southwest China. Our study not only demonstrates the importance of using a fractional spatial rescaling method, but also illustrates the value of annually resolved LULC time series for detecting significant trends and transitions in LULCC, thus potentially facilitating a more robust use of remotely sensed data in land-atmosphere interaction studies

Effects of Training Set Size on Supervised Machine-Learning Land-Cover Classification of Large-Area High-Resolution Remotely Sensed Data

The size of the training data set is a major determinant of classification accuracy. Neverthe- less, the collection of a large training data set for supervised classifiers can be a challenge, especially for studies covering a large area, which may be typical of many real-world applied projects. This work investigates how variations in training set size, ranging from a large sample size (n = 10,000) to a very small sample size (n = 40), affect the performance of six supervised machine-learning algo- rithms applied to classify large-area high-spatial-resolution (HR) (1–5 m) remotely sensed data within the context of a geographic object-based image analysis (GEOBIA) approach. GEOBIA, in which adjacent similar pixels are grouped into image-objects that form the unit of the classification, offers the potential benefit of allowing multiple additional variables, such as measures of object geometry and texture, thus increasing the dimensionality of the classification input data. The six supervised machine-learning algorithms are support vector machines (SVM), random forests (RF), k-nearest neighbors (k-NN), single-layer perceptron neural networks (NEU), learning vector quantization (LVQ), and gradient-boosted trees (GBM). RF, the algorithm with the highest overall accuracy, was notable for its negligible decrease in overall accuracy, 1.0%, when training sample size decreased from 10,000 to 315 samples. GBM provided similar overall accuracy to RF; however, the algorithm was very expensive in terms of training time and computational resources, especially with large training sets. In contrast to RF and GBM, NEU, and SVM were particularly sensitive to decreasing sample size, with NEU classifications generally producing overall accuracies that were on average slightly higher than SVM classifications for larger sample sizes, but lower than SVM for the smallest sample sizes. NEU however required a longer processing time. The k-NN classifier saw less of a drop in overall accuracy than NEU and SVM as training set size decreased; however, the overall accuracies of k-NN were typically less than RF, NEU, and SVM classifiers. LVQ generally had the lowest overall accuracy of all six methods, but was relatively insensitive to sample size, down to the smallest sample sizes. Overall, due to its relatively high accuracy with small training sample sets, and minimal variations in overall accuracy between very large and small sample sets, as well as relatively short processing time, RF was a good classifier for large-area land-cover classifications of HR remotely sensed data, especially when training data are scarce. However, as performance of different supervised classifiers varies in response to training set size, investigating multiple classification algorithms is recommended to achieve optimal accuracy for a project

Spatiotemporal Evolution of Land Subsidence in the Beijing Plain 2003–2015 Using Persistent Scatterer Interferometry (PSI) with Multi-Source SAR Data

Land subsidence is one of the most important geological hazards in Beijing, China, and its scope and magnitude have been growing rapidly over the past few decades, mainly due to long-term groundwater withdrawal. Interferometric Synthetic Aperture Radar (InSAR) has been used to monitor the deformation in Beijing, but there is a lack of analysis of the long-term spatiotemporal evolution of land subsidence. This study focused on detecting and characterizing spatiotemporal changes in subsidence in the Beijing Plain by using Persistent Scatterer Interferometry (PSI) and geographic spatial analysis. Land subsidence during 2003–2015 was monitored by using ENVISAT ASAR (2003–2010), RADARSAT-2 (2011–2015) and TerraSAR-X (2010–2015) images, with results that are consistent with independent leveling measurements. The radar-based deformation velocity ranged from −136.9 to +15.2 mm/year during 2003–2010, and −149.4 to +8.9 mm/year during 2011–2015 relative to the reference point. The main subsidence areas include Chaoyang, Tongzhou, Shunyi and Changping districts, where seven subsidence bowls were observed between 2003 and 2015. Equal Fan Analysis Method (EFAM) shows that the maximum extensive direction was eastward, with a growing speed of 11.30 km2/year. Areas of differential subsidence were mostly located at the boundaries of the seven subsidence bowls, as indicated by the subsidence rate slope. Notably, the area of greatest subsidence was generally consistent with the patterns of groundwater decline in the Beijing Plain