Effect of Phosphorus Nutrition on Growth and Physiology of Cotton Under Ambient and Elevated Carbon Dioxide

Phosphorous deficiency in soil limits crop growth and productivity in the majority of arable lands worldwide and may moderate the growth enhancement effect of rising atmospheric carbon dioxide (CO2) concentration. To evaluate the interactive effect of these two factors on cotton (Gossypium hirsutum) growth and physiology, plants were grown in controlled environment growth chambers with three levels of phosphate (Pi) supply (0.20, 0.05 and 0.01 mM) under ambient and elevated (400 and 800 μmol mol‒1, respectively) CO2. Phosphate stress caused stunted growth and resulted in early leaf senescence with severely decreased leaf area and photosynthesis. Phosphate stress led to over 77 % reduction in total biomass across CO2 levels. There was a below-ground (roots) shift in biomass partitioning under Pi deficiency. While tissue phosphorus (P) decreased, tissue nitrogen (N) content tended to increase under Pi deficiency. The CO2 × Pi interactions were significant on leaf area, photosynthesis and biomass accumulation. The stimulatory effect of elevated CO2 on growth and photosynthesis was reduced or highly depressed suggesting an increased sensitivity of cotton to Pi deficiency under elevated CO2. Although, tissue P and stomatal conductance were lower at elevated CO2, these did not appear to be the main causes of cotton unresponsiveness to elevated CO2 under severe Pi-stress. The alteration in the uptake and utilization of N was suggested due to a consistent reduction (18–21 %) in the cotton plant tissue N content under elevated CO2

Statistical Analysis of Microarray Data with Replicated Spots: A Case Study with Synechococcus WH8102

Until recently microarray experiments often involved relatively few arrays with only a single representation of each gene on each array. A complete genome microarray with multiple spots per gene (spread out spatially across the array) was developed in order to compare the gene expression of a marine cyanobacterium and a knockout mutant strain in a defined artificial seawater medium. Statistical methods were developed for analysis in the special situation of this case study where there is gene replication within an array and where relatively few arrays are used, which can be the case with current array technology. Due in part to the replication within an array, it was possible to detect very small changes in the levels of expression between the wild type and mutant strains. One interesting biological outcome of this experiment is the indication of the extent to which the phosphorus regulatory system of this cyanobacterium affects the expression of multiple genes beyond those strictly involved in phosphorus acquisition

The ABCD of usability testing

We introduce a methodology for tracking and auditing feedback, errors and suggestions for software packages. This short paper describes how we innovate on the evaluation mechanism, introducing an (Antecedent, Barrier, Consequence and Development) ABCD form, embedded within an eParticipation platform to enable end users to easily report on any usability issues. This methodology will be utilised to improve the STEP cloud eParticipation platform (part of the current STEP Horizon2020 project http://step4youth.eu. The platform is currently being piloted in real life contexts, with the participation of public authorities that are integrating the eParticipation platform into their regular decision-making practices. The project is involving young people, through engagement and motivation strategies and giving them a voice in Environmental decision making at the local level. The pilot evaluation aims to demonstrate how open engagement needs to be embedded within public sector processes and the usability methodology reported here will help to identify the key barriers for wide scale deployment of the platform

Sensitive Chandra coverage of a representative sample of weak-line quasars::revealing the full range of X-ray properties

We present deeper Chandra observations for weak-line quasars (WLQs) in a representative sample that previously had limited X-ray constraints, and perform X-ray photometric analyses to reveal the full range of X-ray properties of WLQs. Only 5 of the 32 WLQs included in this representative sample remain X-ray undetected after these observations, and a stacking analysis shows that these 5 have an average X-ray weakness factor of > 85. One of the WLQs in the sample that was known to have extreme X-ray variability, SDSS J1539+3954, exhibited dramatic X-ray variability again: it changed from an X-ray normal state to an X-ray weak state within ~ 3 months in the rest frame. This short timescale for an X-ray flux variation by a factor of $\gtrsim$ 9 further supports the thick disk and outflow (TDO) model proposed to explain the X-ray and multiwavelength properties of WLQs. The overall distribution of the X-ray-to-optical properties of WLQs suggests that the TDO has an average covering factor of the X-ray emitting region of ~ 0.5, and the column density of the TDO can range from $N_{\rm H}$ $\sim 10^{23-24}~{\rm cm}^{-2}$ to $N_{\rm H}$ $\gtrsim 10^{24}~{\rm cm}^{-2}$, which leads to different levels of absorption and Compton reflection (and/or scattering) among WLQs.Comment: 16 pages, 10 figures. Accepted for publication in MNRA

Broad absorption line disappearance/emergence in multiple ions in a weak emission-line quasar

We report the discovery of disappearance of Mg ii, Al iii, C iv, and Si iv broad absorption lines (BALs) at the same velocity, accompanied by a new Civ BAL emerging at a higher velocity, in the quasar J0827+4252 at z = 2.038. This is the first report of BAL disappearance (i) over Mg ii, Al iii, C iv, and Si iv ions and (ii) in a weak emission-line quasar (WLQ). The discovery is based on four spectra from the SDSS and one follow-up spectrum from HET/LRS2. The simultaneous Civ BAL disappearance and emergence at different velocities, together with no variations in the CRTS light curve, indicate that ionization changes in the absorbing material are unlikely to cause the observed BAL variability. Our analyses reveal that transverse motion is the most likely dominant driver of the BAL disappearance/emergence. Given the presence of mildly relativistic BAL outflows and an apparently large C iv emission-line blueshift that is likely associated with strong bulk outflows in this WLQ, J0827+4252 provides a notable opportunity to study extreme quasar winds and their potential in expelling material from inner to large-scale regions.Comment: Published in 2019 ApJL, 870,