Optimal photometry for colour-magnitude diagrams and its application to NGC 2547

We have developed the techniques required to use the optimal photometry algorithm of Naylor (1998) to create colour-magnitude diagrams with well defined completeness functions. To achieve this we first demonstrate that the optimal extraction is insensitive to uncertainties in the star's measured position. We then show how to correct the optimally extracted fluxes such that they correspond to those measured in a large aperture, so aperture photometry of standard stars can be used to place the measurements on a standard system. The technique simultaneously removes the effects of a position dependent point-spread function. Finally we develop a method called "ghosting", which calculates the completeness corrections in the absence of an accurate description of the point spread function. We apply these techniques to the young cluster NGC 2547 (=C0809-491), and use an X-ray selected sample to find an age of 20-35Myr and an intrinsic distance modulus of 8.00-8.15 magnitudes. We use these isochrones to select members from our photometric surveys. Our derived luminosity function shows a well defined Wielen dip, making NGC 2547 the youngest cluster in which such a feature has been observed. Our derived mass function spans the range 0.1-6Mo and is similar to that for the field and the older, more massive clusters M35 and the Pleiades, supporting the idea of a universal initial mass function.Comment: 24 pages, 20 figures, MNRAS accepte

Path-Breakers: How Does Women's Political Participation Respond to Electoral Success?

This paper analyzes the effect of a woman's electoral victory on women's subsequent political participation. Using the regression discontinuity afforded by close elections between women and men in India's state elections, we find that a woman winning office leads to a large and significant increase in the share of female candidates from major political parties in the subsequent election. This stems mainly from an increased probability that previous women candidates contest again, an important margin in India where a substantial number of incumbents do not contest re-election. There is no significant entry of new female candidates, no change in female or male voter turnout and no spillover effects to neighboring areas. Further analysis points to a reduction in party bias against women candidates as the main mechanism driving the observed increase in women's candidacy

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Fluorine-18 Labeled Urea-Based Ligands Targeting Prostate-Specific Membrane Antigen (PSMA) with Increased Tumor and Decreased Renal Uptake

High expression of prostate-specific membrane antigen (PSMA) in prostate cancers prompted the development of the PSMA-targeted PET-imaging agent [18F]DCFPyL, which was recently approved by the FDA. Fluorine-18-labeled Lys–Urea–Glu-based oxime derivatives of [18F]DCFPyL were prepared for the comparison of their in vitro and in vivo properties to potentially improve kidney clearance and tumor targeting. The oxime radiotracers were produced by condensation of an aminooxy functionalized PSMA-inhibitor Lys–Urea–Glu scaffold with fluorine-18-labeled aldehydes. The radiochemical yields were between 15–42% (decay uncorrected) in 50–60 min. In vitro saturation and competition binding assays with human prostate cancer cells transfected with PSMA, PC3(+), indicated similar high nM binding affinities to PSMA for all radiotracers. In vivo biodistribution studies with positive control PC3(+) tumor xenografts showed that the kidneys had the highest uptake followed by tumors at 60 min. The PC3(+) tumor uptake was blocked with non-radioactive DCFPyL, and PC3(−) tumor xenograft (negative control) tumor uptake was negligible indicating that PSMA targeting was preserved. The most lipophilic tracer, [18F]2a, displayed comparable tumor-targeting to [18F]DCFPyL and a desirable alteration in pharmacokinetics and metabolism, resulting in significantly lower kidney uptake with a shift towards hepatobiliary clearance and increased liver uptake

Primary Amine Stabilization of a Dicopper(III) Bis(μ-oxo) Species: Modeling the Ligation in pMMO

Here we report the formation of the first examples of dicopper­(III) bis­(μ-oxo) complexes ligated by the primary amines, propylenediamine, and <i>N</i>,<i>N</i>,-dimethyl propylenediamine. Stabilization of these new compounds is effected at −125 °C by “core capture” introduction of exogenous ligand to a preformed dicopper­(III) bis­(μ-oxo) complex supported by the peralkylated tetramethyl propylenediamine. Primary amine ligation in these compounds matches the single primary amine coordination of the putative active site of particulate methane monooxygenase (pMMO) and polysaccharide monooxygenase. Reactivity studies presented here show primary amine ligated cores are competent oxidants, capable of activating C–H bonds by an H-atom abstraction mechanism. Trends in spectroscopy, structure, and reactivity provide hints to the potential role of primary amine ligation in pMMO: increased substrate accessibility to the redox active orbitals of the Cu₂O₂ core and greater stabilization of the oxidant without attenuation of oxidizing power

Adapting Urban Water Systems to a Changing Climate: Lessons from the Millennium Drought in Southeast Australia

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