Localisations of cobordism categories and invertible TFTs in dimension two

Cobordism categories have played an important role in classical geometry and more recently in mathematical treatments of quantum field theory. Here we will compute localisations of two-dimensional discrete cobordism categories. This allows us, up to equivalence, to determine the category of invertible two-dimensional topological field theories in the sense of Atiyah. We are able to treat the orientable, non-orientable, closed and open cases.Comment: 30 pages, accepted for publication by Homology, Homotopy and Application

Supply chain integration in New Zealand: benchmark comparisons with the UK automotive sector

Supply chain integration is a promising approach to cross-enterprise process improvement that is still not well understood. This research investigates the level of sophistication (maturity) of supply chain integration in New Zealand from the systems uncertainty perspective. Uncertainty levels of value streams are evaluated using the 'uncertainty circle' concept, thereby enabling benchmark comparisons of value stream performance. A sample of 21 NZ process industry value streams is assessed using a so-called Quick Scan Audit Methodology (QSAM). and the uncertainty results compared with those obtained from 21 value streams in the UK automotive sector. This benchmarking revealed that value streams in New Zealand are weakly integrated and have control mechanisms which are significantly looser than those of the UK sample, even though they face higher uncertainty on the control and demand sides. In contrast, crosscountry differences in supply and process uncertainty are marginal. While providing insights into the general health of New Zealand value streams, the authors acknowledge that the sample is not a comprehensive representation of every NZ value stream

Administration of galacto-oligosaccharide prebiotics in the Flinders Sensitive Line animal model of depression

INTRODUCTION: Major depressive disorder is the leading source of disability globally and current pharmacological treatments are less than adequate. Animal models such as the Flinders Sensitive Line (FSL) rats are used to mimic aspects of the phenotype in the human disorder and to characterise candidate antidepressant agents. Communication between the gut microbiome and the brain may play an important role in psychiatric disorders such as depression. Interventions targeting the gut microbiota may serve as potential treatments for depression, and this drives increasing research into the effect of probiotics and prebiotics in neuropsychiatric disorders. Prebiotics, galacto-oligosaccharides and fructooligosaccharides that stimulate the activity of gut bacteria have been reported to have a positive impact, reducing anxiety and depressive-like phenotypes and stress-related physiology in mice and rats, as well as in humans. Bimuno, the commercially available beta-galacto-oligosaccharide, has been shown to increase gut microbiota diversity. AIM: Here, we aim to investigate the effect of Bimuno on rat anxiety-like and depressive-like behaviour and gut microbiota composition in the FSL model, a genetic model of depression, in comparison to their control, the Flinders Resistant Line (FRL) rats. METHODS: Sixty-four male rats aged 5–7 weeks, 32 FSL and 32 FRL rats, will be randomised to receive Bimuno or control (4 g/kg) daily for 4 weeks. Animals will be tested by an experimenter unaware of group allocation on the forced swim test to assessed depressive-like behaviour, the elevated plus maze to assess anxiety-like behaviour and the open field test to assess locomotion. Animals will be weighed and food and water intake, per kilogram of bodyweight, will be recorded. Faeces will be collected from each animal prior to the start of the experiment and on the final day to assess the bacterial diversity and relative abundance of bacterial genera in the gut. All outcomes and statistical analysis will be carried out blinded to group allocation, group assignments will be revealed after raw data have been uploaded to Open Science Framework. Two-way analysis of variance will be carried out to investigate the effect of treatment (control or prebiotic) and strain (FSL or FRL) on depressive-like and anxiety-like behaviours

Artificial Metalloenzymes Based on the Biotin–Streptavidin Technology: Challenges and Opportunities

The biotin–streptavidin technology offers an attractive means to engineer artificial metalloenzymes (ArMs). Initiated over 50 years ago by Bayer and Wilchek, the biotin–(strept)avidin techonology relies on the exquisite supramolecular affinity of either avidin or streptavidin for biotin. This versatile tool, commonly referred to as “molecular velcro”, allows nearly irreversible anchoring of biotinylated probes within a (strept)avidin host protein. Building upon a visionary publication by Whitesides from 1978, several groups have been exploiting this technology to create artificial metalloenzymes. For this purpose, a biotinylated organometallic catalyst is introduced within (strept)avidin to afford a hybrid catalyst that combines features reminiscent of both enzymes and organometallic catalysts. Importantly, ArMs can be optimized by chemogenetic means. Combining a small collection of biotinylated organometallic catalysts with streptavidin mutants allows generation of significant diversity, thus allowing optimization of the catalytic performance of ArMs. Pursuing this strategy, the following reactions have been implemented: hydrogenation, alcohol oxidation, sulfoxidation, dihydroxylation, allylic alkylation, transfer hydrogenation, Suzuki cross-coupling, C–H activation, and metathesis. In this Account, we summarize our efforts in the latter four reactions. X-ray analysis of various ArMs based on the biotin–streptavidin technology reveals the versatility and commensurability of the biotin-binding vestibule to accommodate and interact with transition states of the scrutinized organometallic transformations. In particular, streptavidin residues at positions 112 and 121 recurrently lie in close proximity to the biotinylated metal cofactor. This observation led us to develop a streamlined 24-well plate streptavidin production and screening platform to optimize the performance of ArMs. To date, most of the efforts in the field of ArMs have focused on the use of purified protein samples. This seriously limits the throughput of the optimization process. With the ultimate goal of complementing natural enzymes in the context of synthetic and chemical biology, we outline the milestones required to ultimately implement ArMs within a cellular environment. Indeed, we believe that ArMs may allow signficant expansion of the natural enzymes’ toolbox to access new-to-nature reactivities in vivo . With this ambitious goal in mind, we report on our efforts to (i) activate the biotinylated catalyst precursor upon incorporation within streptavidin, (ii) minimize the effect of the cellular environment on the ArM’s performance, and (iii) demonstrate the compatibility of ArMs with natural enzymes in cascade reactions

Adhesion of surfaces mediated by adsorbed particles: Monte Carlo simulations and a general relationship between adsorption isotherms and effective adhesion energies

Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.In colloidal and biological systems, interactions between surfaces are often mediated by adsorbed particles or molecules that interconnect the surfaces. In this article, we present a general relationship between the adsorption isotherms of the particles and the effective, particle-mediated adhesion energies of the surfaces. Our relationship is based on the analysis and modeling of detailed data from Monte Carlo simulations. As general properties that should hold for a wide class of adsorption scenarios, we find (i) that the particle-mediated adhesion energies of surfaces are maximal at intermediate bulk concentrations of the particles, and (ii) that the particle coverage in the bound state of the surfaces is twice the coverage in the unbound state at these bulk concentrations.DFG, GRK 1524, Self-Assembled Soft-Matter Nanostructures at Interface

Mental health benefits of interactions with nature in children and teenagers: a systematic review

Background It is commonly believed that nature has positive impacts on children’s health, including physical, mental and social dimensions. This review focuses on how accessibility to, exposure to and engagement with nature affects the mental health of children and teenagers. Methods Ten academic databases were used to systematically search and identify primary research papers in English or French from 1990 to 1 March 2017. Papers were included for review based on their incorporation of nature, children and teenagers (0–18 years), quantitative results and focus on mental health. Results Of the 35 papers included in the review, the majority focused on emotional well-being and attention deficit disorder/hyperactivity disorder. Other outcome measures included overall mental health, self-esteem, stress, resilience, depression and health-related quality of life. About half of all reported findings revealed statistically significant positive relationships between nature and mental health outcomes and almost half reported no statistical significance. Conclusions Findings support the contention that nature positively influences mental health; however, in most cases, additional research with more rigorous study designs and objective measures of both nature and mental health outcomes are needed to confirm statistically significant relationships. Existing evidence is limited by the cross-sectional nature of most papers

Contribution of Fdh3 and Glr1 to Glutathione Redox State, Stress Adaptation and Virulence in Candida albicans

Acknowledgments: We thank Aaron Mitchell and Dominique Sanglard for providing the C. albicans protein kinase and transposon mutant libraries, and Louise Walker for the strain CAMY203.Peer reviewedPublisher PD

Intercomparison of Hantzsch and fiber-laser-induced-fluorescence formaldehyde measurements

Two gas-phase formaldehyde (HCHO) measurement techniques, a modified commercial wet-chemical instrument based on Hantzsch fluorimetry and a custom-built instrument based on fiber laser-induced fluorescence (FILIF), were deployed at the atmospheric simulation chamber SAPHIR (Simulation of Atmospheric PHotochemistry In a large Reaction Chamber) to compare the instruments' performances under a range of conditions. Thermolysis of para-HCHO and ozonolysis of 1-butene were used as HCHO sources, allowing for calculations of theoretical HCHO mixing ratios. Calculated HCHO mixing ratios are compared to measurements, and the two measurements are also compared. Experiments were repeated under dry and humid conditions (RH 60%) to investigate the possibility of a water artifact in the FILIF measurements. The ozonolysis of 1-butene also allowed for the investigation of an ozone artifact seen in some Hantzsch measurements in previous intercomparisons. Results show that under all conditions the two techniques are well correlated (R2 ≥ 0.997), and linear regression statistics show measurements agree with within stated uncertainty (15% FILIF + 5% Hantzsch). No water or ozone artifacts are identified. While a slight curvature is observed in some Hantzsch vs. FILIF regressions, the potential for variable instrument sensitivity cannot be attributed to a single instrument at this time. Measurements at low concentrations highlight the need for a secondary method for testing the purity of air used in instrument zeroing and the need for further FILIF White cell outgassing experiments