In vitro and in vivo evaluation of antidiabetic potential of extracts of selected medicinal plant preparations collected from Nigerian traditional medical practitioners

Background: Diabetes mellitus is one of the main chronic diseases worldwide. It is characterized by persistent hyperglycaemia. Individuals with persistent  hyperglycaemia are at high risk of developing complications (blindness, leg  amputations and kidney failure). Nigeria is 1 of 4 highly populated countries in  Africa with the largest number of people with diabetes mellitus majority of who use plant extracts given to them by traditional medical practitioners (TMPs).Objectives: To assess the antidiabetic potential of extracts of selected Nigerian medicinal plant recipes collected from TMPS.Materials and Methods: Eighteen recipes (maximum 2 plants) were selected after an ethnobotanical survey of medicinal plant recipes used by TMPs in the management of diabetes mellitus in the 6 geopolitical zones of Nigeria. Recipes were extracted in water according to traditional usage and screened in vitro to assess glucose uptake in C2C12 muscle cells and glucose production by the H4IIE liver cells (through inhibition of glucose-6-phosphatase, the rate limiting enzyme) and in vivo through the oral glucose tolerance test in normal mice (2 g/kg glucose).Results: Two extract recipes (NC01 and NC09E) stimulated glucose uptake in  C2C12 cells, 5 (NW14, NE15, NC01, SE01 and NW01) reduced glucose production in H4IIE cells and 4 (SE04, SE11, NC01 and NC09E) prevented hyperglycaemia in glucose-loaded normal mice. Conclusion: Only a few of the extracts from the recipes collected from the TMPs showed antidiabetic activity by increasing glucose uptake in the muscle (11%), reducing glucose production in the liver (28%) and/or preventing hyperglycaemia (22%).Keywords: Diabetes mellitus, Recipe extracts, Glucose uptake, Glucose production, Oral glucose tolerance tes

Crinum zeylanicum memory enhancing effect is mediated via central cholinergic transmission system

Background: Crinum zeylanicum is widely used in the ethno-therapeutic management of folk management of epilepsy, pain, neuropsychiatric, and dementing disorders in Nigeria. The current study was carried out to evaluate the possible mechanism of the memory enhancing the effect of C. zeylanicum extract and alkaloidal rich fraction in Wistar rats.Methods: The effect of Crinum zeylanicum bulb extract (250, 500, and 1000 mg/kg body weight orally), alkaloidal rich fraction (10, 20, and 40 mg/kg body weight p.o.), normal saline (10 ml/kg orally), or Eserine (0.3 mg/kg body weight i.p.) on spatial memory in rats was evaluated using the Y-maze. The blood samples obtained from rats in all treatment groups were evaluated for cholinesterase activities using modified Michelle electrometric method.Results: The extract and the alkaloid significantly (p<0.05) and dose-dependently increased spontaneous alternation behavior of rats in Y-maze. The extract produced 20.00%, 35.55%, and 52.00% inhibition of cholinesterase activity in the blood at 250, 500, and 1000 mg/kg body weight, respectively. The alkaloid produced 56.67%, 62.67%, and 68.67% inhibition of cholinesterase activity in blood at 10, 20, and 40 mg/kg body weight (p.o.). Eserine a standard cholinesterase inhibitor at 0.3 mg/kg body weight produced a significant increase in spontaneous alternation behavior and produced 73.33% inhibition of blood cholinesterase activity. Data obtained from the study showed that the enhanced spontaneous alternation behavior observed in rats treated with the extract, and the alkaloid may be due to facilitation of cholinergic transmission resulting from inhibition of cholinesterase activity.Conclusion: The extract, as well as its partially purified alkaloid, possesses potential that may be employed for therapeutic management of Alzheimer’s disease

ANTI-ULCER AND WOUND HEALING ACTIVITIES OF SIDA CORYMBOSA IN RATS

Background: There are strong beliefs in the efficacy of traditional medical systems worldwide. Many herbs have been acclaimed to possess antiulcer effects and could be unexplored sources of new lead compounds. Sida corymbosa R. E. Fries (Malvaceae) is used in Northern Nigeria to treat ulcers and wounds. This work aimed to investigate the usefulness of Sida corymbosa in treatments of stomach ulcers and wounds in traditional medicine. Materials and Methods: Effect of the aqueous extract was determined on gastric ulceration, rate of wound healing and inflammation using ethanol-induced and diclofenac-induced ulceration, wound excision model and albumin-induced inflammation respectively in rats. Results: The study demonstrated the anti-ulcer activity of Sida corymbosa as the extract (250, 500 and 1000 mg/kg) showed a dose-dependent, significant (

Development of a Dynamic Cuckoo Search Algorithm

This research is aimed at the developing a modified cuckoo search algorithm called dynamic cuckoo search algorithm (dCSA). The standard cuckoo search algorithm is a metaheuristics search algorithm that mimic the behavior of brood parasitism of some cuckoo species and Levy flight behavior of some fruit flies and birds. It, however uses fixed value for control parameters (control probability and step size) and this method have drawbacks with respect to quality of the solutions and number of iterations to obtain optimal solution. Therefore, the dCSA is developed to address these problems in the CSA by introducing random inertia weight strategy to the control parameters so as to make the control parameters dynamic with respect to the proximity of a cuckoo to the optimal solution. The developed dCSA was compared with CSA using ten benchmark test functions. The results obtained indicated the superiority of dCSA over CSA by generating a near global optimal result for 9 out of the ten benchmark test functions

Development of Hybrid Automatic Segmentation Technique of a Single Leaf from Overlapping Leaves Image

The segmentation of a single leaf from an image with overlapping leaves is an important step towards the realization of effective precision agricultural systems. A popular approach used for this segmentation task is the hybridization of the Chan-Vese model and the Sobel operator CV-SO. This hybridized approach is popular because of its simplicity and effectiveness in segmenting a single leaf of interest from a complex background of overlapping leaves. However, the manual threshold and parameter tuning procedure of the CV-SO algorithm often degrades its detection performance. In this paper, we address this problem by introducing a dynamic iterative model to determine the optimal parameters for the CV-SO algorithm, which we dubbed the Dynamic CV-SO (DCV-SO) algorithm. This is a new hybrid automatic segmentation technique that attempts to improve the detection performance of the original hybrid CV-SO algorithm by reducing its mean error rate. The results obtained via simulation indicate that the proposed method yielded a 1.23% reduction in the mean error rate against the original CV-SO method

Antiplasmodial Efficacy Of Methanolic Root And Leaf Extracts Of Morinda lucida

Development of antimalarial drugs from medicinal plants continues to be a very appealing option. Morindalucida, commonly known as “Ugigo” by the Ebira people in Kogi State North-Central Nigeria, is used in the treatment of malaria, fever, amongst other ailments. Therapeutic effects of the methanolic root extract and a combination of extracts of the leaf and root parts were evaluated in Plasmodium bergheiinfection in mice. Percentage suppression of parasitaemia for the methanolic root extract was 56.30, 59.84, 67.72 and 81.80% for doses of 100, 200, 400mg/kg body weight of the extract, and 5mg/kg chloroquine respectively. The mean survival period in days were 15.00 ± 0.70, 18.75 ± 0.5, 19.75 ± 1.39, 23.25 ± 1.38 and 8.75 ± 1.25, for 100, 200, 400mg/kg body weight of the extract, 5mg/kg chloroquine, and the untreated control respectively. Effective dose dependent inhibitions of parasitaemia were also observed in the curative test. In the combination study, it was observed that, the antimalarial activity for leaf and root was slightly more, compared to that of each of the extracts, as seen in parasite inhibition, after 5days of treatment (26.00, 20.00, 25.28, 21.35, 27.00, 19.50, 8.5 and 85.00), for 100, 200mg/kg leaf extract alone, 100 and 200mg/kg root extract alone, 50, 100mg/kg leaf and root extracts, 5mg/kg chloroquine and control groups respectively. It is concluded that the methanolic root and leaf extracts of Morindalucida are potentially useful for the development of antimalarial drug. Key words: Antimalaria, Percentage suppression, Morinda lucida, Plasmodium berghei, Parasitaemi

GASTRO-PROTECTIVE EFFECT OF CROSSOPTERYX FEBRIFUGA IN WISTAR RATS.

Preparations of Crossopteryx febrifuga (Afzel.) Benth. (Rubiaceae) are widely used in Northern Nigeria in the therapeutic management of trypanosomiasis, malaria and painful inflammatory disorders. Previous studies have shown that the methanolic stem bark extract of Crossopteryx febrifuga possesses significant analgesic and anti-inflammatory properties possibly mediated via Non-selective inhibition of cyclo-oxygenase pathways. In the present study, the methanolic stem bark extract of Crossopteryx febrifuga was evaluated against ethanol- and piroxicam-induced ulceration in rats. Histopathological studies of the rat stomach tissues were also carried out in order to determine its safety profile on the gastrointestinal tract (git). The extract (25, 50 and100 mg extract/kg body weight) significantly (

Characteristics of COVID-19 cases and factors associated with their mortality in Katsina State, Nigeria, April-July 2020

Introduction: COVID-19 was first detected in Daura, Katsina State, Nigeria on 4 April 2020. We characterized the cases and outlined factors associated with mortality. Methods: We analysed the COVID-19 data downloaded from Surveillance Outbreak Response, Management and Analysis System between 4 April and 31 July 2020. We defined a case as any person with a positive SARS-CoV-2 test within that period. We described the cases in time, person, and place; calculated the crude and adjusted odds ratios and 95% confidence intervals for factors associated with mortality. Results: We analysed 744 confirmed cases (median age 35, range 1-90), 73% males and 24 deaths (Case fatality rate 3.2%, Attack rate 8.5/100,000). The outbreak affected 31 districts, started in week 14, peaked in week 26, and is ongoing. Highest proportion of cases in the age groups were 26.7% (184) in 30-39, 21.7% (153) in 20-29 years, and 18.3% (129) in 40-49 years. While the highest case fatality rates in the age groups were 35.7% in 70-79, 33.3% in 80-89 years, and 19.4% in 60-69 years. Factors associated with death were cough (AOR: 9.88, 95% CI: 1.29-75.79), age ≥60 years (AOR: 18.42, 95% CI: 7.48-45.38), and male sex (AOR: 4.4, 95% CI: 0.98-20.12). Conclusion: Male contacts below 40 years carried the burden of COVID-19. Also, persons 60 years and above, with cough have an increased risk of dying from COVID-19. Risk communication should advocate for use of preventive measures, protection of persons 60 years and above, and consideration of cough as a red-flag sign

Neuropharmacological effects of standardized aqueous stem bark extract of Parkia biglobossa in Wistar rats

Objective: Parkia biglobossa stem bark decoction is a popular medicinal plant preparation used as calming agent for tensed patients in traditional medicine. The aim of this study was to evaluate the effects of aqueous stem bark extract of Parkia biglobossa (AEPB) and its active fraction AEPBF3 on anxiety, spontaneous alternation behavior, and locomotor activity. The open field apparatus was used to evaluate effects of AEPB and AEPBF3 on locomotion.The APBE and the  active fraction AEPBF3 were standardized using reverse phase high performance liquid chromatography to establish finger print to ascertain identity and stability of the extracts over time. Materials and Methods: The oral median lethal doses (LD50) of AEPB and AEPBF3 were evaluated using modified Lorke’s method in rats. The  effect of APBE (50-200 mg/kg p.o.), APBEF3 (25 and 50 mg/kg p.o.), diazepam (2.5 mg/kg, i.p.), and 10 ml normal saline/kg on anxiety-like behavior, spontaneous alternation behavior, and locomotion activity were evaluated in rats on elevated plus maze (EPM), Zero-maze, Y-maze, and open field apparatus, respectively. The oral LD50 values of AEPB and AEPBF3 were estimated to be 5000 mg/kg and 3800 mg/kg body weight in rats, respectively. Results: AEPB and AEPBF3 significantly (F6, 41=2342,

Neuro-pharmacological effects of Crinum zeylanicum in mice

Objectives: The aim of present study was to evaluate some effects of Crinum zeylanicum (C. zeylanicum) on central nervous system. Materials and Methods: C. zeylanicum methanolic bulb extract (250-1000 mg/kg orally), 2 mg chlorpromazine and 4 mg diazepam /kg body weight intraperitoneally respectively were tested in mice using Irwin test, pentobarbitone-induced sleep test, spontaneous motor activity, apomorphine-induced stereotype behaviour, and rota-rod performance. Results: The C. zeylanicum bulb extract significantly and dose-dependently decreased apomorphine-induced hyperactivity in mice (