80 research outputs found
Comments on the development of harmonized method for Sustainability Assessment of Technologies (SAT)
A VAR2CSA:CSP conjugate capable of inducing dual specificity antibody responses
Background: Vaccine antigens targeting specific P. falciparum parasite stages are under pre-clinical and clinical development. It seems plausible that vaccine with multiple specificities will offer higher protection. With this hypothesis, we exploited the Spy- Tag/SpyCatcher conjugation system to make a, post expression, dual antigen conjugate vaccine, comprising two clinically tested antigen candidates (CSP and VAR2CSA).Methods: The DBL1x-DBL2x-ID2a region of VAR2CSA was genetically fused with SpyTag at N-terminus. The full-length CSP antigen was genetically fused to C-terminal SpyCatcher peptide. The covalent interaction between SpyTag/ SpyCatcher enables the formation of DBL1x-DBL2x-ID2a:CSP conjugate vaccine. Immunogenicity and quality of antibody responses induced by the conjugate vaccine, as well as a control CSP-SpyCatcher vaccine, was tested in BALB/c mice.Results: Serum samples obtained from mice immunized with the conjugate vaccine were able to recognize both untagged DBL1x-DBL2x-ID2a as well as CSP antigen. Moreover, the geometric mean anti-CSP antibody titer was 1.9-fold higher in serum (at day 35 and 55 post-first immunization) from mice immunized with the conjugate vaccine, as compared to mice receiving the control vaccine.Conclusion: The data obtained in this study serves as proof-of-concept for the simultaneous induction of antibodies directed against individual antigen components in a dual stage anti-malaria vaccine.Keywords: Malaria vaccine, Circumsporozoite protein, VAR2CSA, CSP SpyCatcher, SpyTag-DBL1x-DBL2x-ID2a, bacterial superglue, DBL1x-DBL2x-ID2a:CSP conjugat
A VAR2CSA:CSP conjugate capable of inducing dual specificity antibody responses.
Background: Vaccine antigens targeting specific P. falciparum
parasite stages are under pre-clinical and clinical development. It
seems plausible that vaccine with multiple specificities will offer
higher protection. With this hypothesis, we exploited the
SpyTag/SpyCatcher conjugation system to make a, post expression, dual
antigen conjugate vaccine, comprising two clinically tested antigen
candidates (CSP and VAR2CSA). Methods: The DBL1x-DBL2x-ID2a region of
VAR2CSA was genetically fused with SpyTag at N-terminus. The
full-length CSP antigen was genetically fused to C-terminal SpyCatcher
peptide. The covalent interaction between SpyTag/SpyCatcher enables the
formation of DBL1x-DBL2x-ID2a:CSP conjugate vaccine. Immunogenicity and
quality of antibody responses induced by the conjugate vaccine, as well
as a control CSP-SpyCatcher vaccine, was tested in BALB/c mice.
Results: Serum samples obtained from mice immunized with the conjugate
vaccine were able to recognize both untagged DBL1x-DBL2x-ID2a as well
as CSP antigen. Moreover, the geometric mean anti-CSP antibody titer
was 1.9-fold higher in serum (at day 35 and 55 post-first immunization)
from mice immunized with the conjugate vaccine, as compared to mice
receiving the control vaccine. Conclusion: The data obtained in this
study serves as proof-of-concept for the simultaneous induction of
antibodies directed against individual antigen components in a dual
stage anti-malaria vaccine
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