Genetic characterization of human coxsackievirus A6 variants associated with atypical hand, foot and mouth disease: a potential role of recombination in emergence and pathogenicity

Human coxsackievirus A6 (CVA6) is an enterically transmitted enterovirus. Until recently, CVA6 infections were considered as being of minor clinical significance, and only rarely aetiologically linked with hand, foot and mouth disease (HFMD) associated with other species A enteroviruses (particularly EV71 and CVA16). From 2008 onwards, however, CVA6 infections have been associated with several outbreaks worldwide of atypical HFMD (aHFMD) accompanied by a varicelliform rash. We recently reported CVA6-associated eczema herpeticum occurring predominantly in children and young adults in Edinburgh in January and February 2014. To investigate genetic determinants of novel clinical phenotypes of CVA6, we genetically characterized and analysed CVA6 variants associated with eczema herpeticum in Edinburgh in 2014 and those with aHFMD in CAV isolates collected from 2008. A total of eight recombinant forms (RFs) have circulated worldwide over the past 10 years, with the particularly recent appearance of RF-H associated with eczema herpeticum cases in Edinburgh in 2014. Comparison of phylogenies and divergence of complete genome sequences of CVA6 identified recombination breakpoints in 2A-2C, within VP3, and between 5' untranslated region and VP1. A Bayesian temporal reconstruction of CVA6 evolution since 2004 provided estimates of dates and the actual recombination events that generated more recently appearing recombination groups (RF-E, -F, -G and -H). Associations were observed between recombination groups and clinical presentations of herpangina, aHFMD and eczema herpeticum, but not with VP1 or other structural genes. These observations provided evidence that NS gene regions may potentially contribute to clinical phenotypes and outcomes of CVA6 infection

Remote sensing in Iowa agriculture: Identification and classification of Iowa's crops, soils and forestry resources using ERTS-1 and complimentary underflight imagery

The author has identified the following significant results. Springtime ERTS-1 imagery covering pre-selected test sites in Iowa showed considerable detail with respect to broad soil and land use patterns. Additional imagery has been incorporated into a state mosaic. The mosaic was used as a base for soil association lines transferred from an existing map. The regions of greatest contrast are between the Clarion-Nicollet-Webster soil association area and adjacent areas. Landscape characteristics in this area result in land use patterns with a high percentage of pasture, hay, and timber. The soil association areas of the state that have patterns interpreted to be associated with intensive row crop production are: Moody, Galva-Primghar-Sac, Clarion-Nicollet-Webter, Tama-Muscatine, Dinsdale-Tama, Cresco-Lourdes, Clyde, Kenyon-Floyd-Clyde, and the Luton-Onawa-Salix area on the Missouri River floodplain. Forestland estimates have been attained for an area in central Iowa using wintertime ERTS-1 imagery. Visual analysis of multispectral, temporal imagery indicates that temporal analysis for cropland identification and acreage analyses procedures may be a very useful tool. Combinations of wintertime, springtime, and summertime ERTS-1 imagery separate most vegetation types. Timing can be critical depending upon crop development and harvesting times because of the dynamic nature of agricultural production

Remote sensing in Iowa agriculture: Identification and classification of Iowa's crops, soils and forestry resources using ERTS-1 and complimentary underflight imagery

There are no author-identified significant results in this report

Reduced healthcare utilisation following successful HCV treatment in HIV co-infected patients with mild liver disease

New direct-acting antivirals (DAA) for hepatitis C virus (HCV) infection have achieved high cure rates in many patient groups previously considered difficult-to-treat, including those HIV/HCV co-infected. The high price of these medications is likely to limit access to treatment, at least in the short term. Early treatment priority is likely to be given to those with advanced disease, but a more detailed understanding of the potential benefits in treating those with mild disease is needed. We hypothesized that successful HCV treatment within a co-infected population with mild liver disease would lead to a reduction in the use and costs of healthcare services in the 5 years following treatment completion. We performed a retrospective cohort study of HIV/HCV-co-infected patients without evidence of fibrosis/cirrhosis who received a course of HCV therapy between 2004 and 2013. Detailed analysis of healthcare utilization up to 5 years following treatment for each patient using clinical and electronic records was used to estimate healthcare costs. Sixty-three patients were investigated, of whom 48 of 63 (76.2%) achieved sustained virological response 12 weeks following completion of therapy (SVR12). Individuals achieving SVR12 incurred lower health utilization costs (£5000 per-patient) compared to (£10 775 per-patient) non-SVR patients in the 5 years after treatment. Healthcare utilization rates and costs in the immediate 5 years following treatment were significantly higher in co-infected patients with mild disease that failed to achieve SVR12. These data suggest additional value to achieving cure beyond the prevention of complications of disease

The functional integral with unconditional Wiener measure for anharmonic oscillator

In this article we propose the calculation of the unconditional Wiener measure functional integral with a term of the fourth order in the exponent by an alternative method as in the conventional perturbative approach. In contrast to the conventional perturbation theory, we expand into power series the term linear in the integration variable in the exponent. In such a case we can profit from the representation of the integral in question by the parabolic cylinder functions. We show that in such a case the series expansions are uniformly convergent and we find recurrence relations for the Wiener functional integral in the $N$ - dimensional approximation. In continuum limit we find that the generalized Gelfand - Yaglom differential equation with solution yields the desired functional integral (similarly as the standard Gelfand - Yaglom differential equation yields the functional integral for linear harmonic oscillator).Comment: Source file which we sent to journa

Education pathways to the labour market for 16-year-olds who struggle to achieve maths and English in General Certificate of Secondary Education

We examine the post age-16 educational pathways taken by the 44% of young people who do not gain “good” grades in English and Maths at age 16 years. We then assess the causal effects of attending General Further Education (GFE) colleges on education and labour market outcomes for this group. We use the Longitudinal Education Outcomes dataset, which comprises linked administrative education, employment and income records for the population of English school pupils aged 16 in 2011. To summarise complex post-16 education trajectories, we present Sankey charts stratified by indicators of disadvantage. We study the effects of attending GFE at age 17 on whether a pupil gains a Level 3 qualification by age 19, and their earnings and employment status at age 24. To estimate a causal impact, we use distance from home to the closest GFE college as an instrumental variable, controlling for a rich set of background characteristics. Our graphical results highlight the complexity of post-16 educational pathways and transitions, which are differentiated by disadvantage. Over 50% have GFE as their first post-16 destination. Results from instrumental variable analyses show a positive association between attending GFE and gaining a Level 3 qualification by age 19, among pupils who do not gain a “good” pass in the General Certificate of Secondary Education (GCSE) in either English and/or Maths. Restricting analyses to the bottom of the distribution – those who gain an E, F or G grade in both English and Maths - we do not detect an impact of GFE on qualifications at age 19. Among both subgroups, we do not detect any impact of attending GFE on earnings and employment at age 24 years. While the post-16 pathways taken by disadvantaged, lower-attaining pupils do increase qualification attainment for some, the value these have in the labour market appears limited. These results may indicate the importance of “soft-skills” and early employment experiences for this subgroup of lower-attainers

Extremely low long‐term erosion rates around the Gamburtsev Mountains in interior East Antarctica

The high elevation and rugged relief (>3 km) of the Gamburtsev Subglacial Mountains (GSM) have long been considered enigmatic. Orogenesis normally occurs near plate boundaries, not cratonic interiors, and large‐scale tectonic activity last occurred in East Antarctica during the Pan‐African (480–600 Ma). We sampled detrital apatite from Eocene sands in Prydz Bay at the terminus of the Lambert Graben, which drained a large pre‐glacial basin including the northern Gamburtsev Mountains. Apatite fission‐track and (U‐Th)/He cooling ages constrain bedrock erosion rates throughout the catchment. We double‐dated apatites to resolve individual cooling histories. Erosion was very slow, averaging 0.01–0.02 km/Myr for >250 Myr, supporting the preservation of high elevation in interior East Antarctica since at least the cessation of Permian rifting. Long‐term topographic preservation lends credence to postulated high‐elevation mountain ice caps in East Antarctica since at least the Cretaceous and to the idea that cold‐based glaciation can preserve tectonically inactive topography

Some Results of the Educational Experiment APIS (Cervantes Mission on Board ISS)

Some results of the analysis of the pictures taken along the performance of the Análisis de Propiedades Inerciales de Sólidos, Analysis of the Inertia Properties of Solid Bodies (APIS) experiment carried out in the Cervantes mission on board ISS, are presented. APIS was an educational experiment devoted to take advantage of the unique conditions of absence of relative gravity forces of a space platform such as ISS, to show some of the characteristics of the free rotational motion of a solid body, which are impossible to carry out on earth. This field of experimental research has application to aerospace engineering science (e.g. attitude control of spacecrafts), to astrophysical sciences (e.g. state of rotation and tumbling motions of asteroids) and to engineering education. To avoid the effect of the ambient atmosphere loads on the motion, the test body is placed inside a sphere, which reduces the effect of the aerodynamic forces to just friction. The drastic reduction of the effect of the surrounding air during the short duration of the experimental sequences allows us to compare the actual motion with the known solutions for the solid body rotation in vacuum. In this paper, some selected, relevant sequences of the sphere enclosing a body with a nominal cylindrical inertia tensor, put into rotation by the astronaut, are shown; the main problems to extract the information concerning the characteristic parameters of the motion are outlined, and some of the results obtained concerning the motion of the test probe are included, which show what seems to be a curious and unexpected solution of the Euler equations for the solid body rotation in vacuum, without energy dissipation, when the angular momentum is almost perpendicular to the axisymmetry axis

Vibrations of a Columnar Vortex in a Trapped Bose-Einstein Condensate

We derive a governing equation for a Kelvin wave supported on a vortex line in a Bose-Einstein condensate, in a rotating cylindrically symmetric parabolic trap. From this solution the Kelvin wave dispersion relation is determined. In the limit of an oblate trap and in the absence of longitudinal trapping our results are consistent with previous work. We show that the derived Kelvin wave dispersion in the general case is in quantitative agreement with numerical calculations of the Bogoliubov spectrum and offer a significant improvement upon previous analytical work.Comment: 5 pages with 1 figur