Success of several programed AI-breeding protocols including ovsynch

In Experiment 1, four programmed AIbreeding treatments were tested. The so-called OvSynch program, which requires no heat detection before a fixed-time insemination, decreased conception rates compared with a similar treatment in which inseminations occurred after detected estrus (30 vs 51%). The traditional two-injection prostaglandin program produced greater conception rate for cattle inseminated after a detected estrus (53%) than after one fixed-time insemination was given in the absence of estrus (31%). A similar protocol of two prostaglandin injections plus an injection of gonadotropin-releasing hormone (GnRH or Cystorelin®) before one fixed-time insemination produced lower conception rates (33%) than when cattle were inseminated after detected estrus (53%). In Experiment 2, the OvSynch program was retested with the interval between the PGF and the second GnRH 2a injection being 48 hr (36 hr in Experiment 1). Conception in 27 cows on the OvSynch48 program with timed insemination (37%) was comparable with 43% in 21 cows on a similar program without the second GnRH injection but inseminated at estrus

Cross-Platform Normalization of Microarray and Rna-Seq Data for Machine Learning Applications

Large, publicly available gene expression datasets are often analyzed with the aid of machine learning algorithms. Although RNA-seq is increasingly the technology of choice, a wealth of expression data already exist in the form of microarray data. If machine learning models built from legacy data can be applied to RNA-seq data, larger, more diverse training datasets can be created and validation can be performed on newly generated data. We developed Training Distribution Matching (TDM), which transforms RNA-seq data for use with models constructed from legacy platforms. We evaluated TDM, as well as quantile normalization, nonparanormal transformation, and a simple log 2 transformation, on both simulated and biological datasets of gene expression. Our evaluation included both supervised and unsupervised machine learning approaches. We found that TDM exhibited consistently strong performance across settings and that quantile normalization also performed well in many circumstances. We also provide a TDM package for the R programming language

P17. P3b event-related potentials show changes in varsity football players due to accumulated sub-concussive head impacts

BACKGROUND: Concussion has been a focus in football at all levels of participation. However, there is a growing appreciation that repetitive sub-concussive impacts may have more significant effects on overall neurological health than the isolated diagnosed concussions that have been the focus of recent research. The purpose of this study was to evaluate the relationship between the number of head impacts that players experience throughout the season and their P300 evoked potential. METHODS: Canadian university football players (n=45) were separated into three groups based on player mass and position/skill (small-skilled, big-skilled and big-unskilled). Groups were separated into low and high levels of impact exposure based on the total number of head impacts experienced in-season. Players completed baseline, midseason, postseason, and follow-up neurophysiological tests to measure P300 evoked potentials. Statistically significant differences between high versus low impact subgroups for each player group were assessed using independent-samples t-tests. RESULTS: Small-skilled and big-skilled players showed statistically significant decreases in P300 amplitude at midseason and postseason for high impact players compared to low impact players. Follow-up measures revealed that all groups were not significantly different compared to baseline measures. DISCUSSION & CONCLUSION: Players that experience a large number of head impacts in varsity football demonstrate significant decreases in specific EEG measures of cognitive function and information processing. INTERDISCIPLINARY REFLECTION: The combination of biomechanical head impact exposure with neurophysiological outcomes yields insight into the processes behind head impacts and their effects on the human brain

Bulk Enthalpy Calculations in the Arc Jet Facility at NASA ARC

The Arc Jet Facilities at NASA Ames Research Center generate test streams with enthalpies ranging from 5 MJ/kg to 25 MJ/kg. The present work describes a rigorous method, based on equilibrium thermodynamics, for calculating the bulk enthalpy of the flow produced in two of these facilities. The motivation for this work is to determine a dimensionally-correct formula for calculating the bulk enthalpy that is at least as accurate as the conventional formulas that are currently used. Unlike previous methods, the new method accounts for the amount of argon that is present in the flow. Comparisons are made with bulk enthalpies computed from an energy balance method. An analysis of primary facility operating parameters and their associated uncertainties is presented in order to further validate the enthalpy calculations reported herein

Synchronization of estrus and ovulation in dairy heifers using norgestomet, GnRH, and PGF2α

Two experiments were performed using the same treatments. All heifers received two injections of PGF2α 14 days apart. Controls then were inseminated after detected estrus. Heifers assigned to the two treatments also received 6 mg of norgestomet for 8 days beginning 7 days before the second of two PGF2α injections. The heifers in the last treatment also received GnRH 48 hr after the second PGF2α injection to induce ovulation in any heifer not observed in estrus before a fixed-time insemination at 72 hr after PGF2α. In Experiment 1, any control heifer or herifer in the two treatments not detected in estrus by 72 hr after PGF2α received a fixed-time insemination at 72 hr. Heifers receiving GnRH tended to have fewer standing events and a shorter duration of estrus. Fixed-time inseminations reduced conception compared to those after detected estrus. In Experiment 2, when inseminations were performed only after detected estrus, all measures of fertility were unaffected by treatments. These results indicated that addition of norgestomet and(or) GnRH did not improve measures of estrus synchronization or fertility of dairy heifers.; Dairy Day, 1997, Kansas State University, Manhattan, KS, 1997

The test case of HD26965: difficulties disentangling weak Doppler signals from stellar activity

We report the discovery of a radial velocity signal that can be interpreted as a planetary-mass candidate orbiting the K dwarf HD26965, with an orbital period of 42.364$\pm$0.015 days, or alternatively, as the presence of residual, uncorrected rotational activity in the data. Observations include data from HIRES, PFS, CHIRON, and HARPS, where 1,111 measurements were made over 16 years. Our best solution for HD26965 $b$ is consistent with a super-Earth that has a minimum mass of 6.92$\pm$0.79 M$_{\oplus}$ orbiting at a distance of 0.215$\pm$0.008 AU from its host star. We have analyzed the correlation between spectral activity indicators and the radial velocities from each instrument, showing moderate correlations that we include in our model. From this analysis, we recover a $\sim$38 day signal, which matches some literature values of the stellar rotation period. However, from independent Mt. Wilson HK data for this star, we find evidence for a significant 42 day signal after subtraction of longer period magnetic cycles, casting doubt on the planetary hypothesis for this period. Although our statistical model strongly suggests that the 42-day signal is Doppler in origin, we conclude that the residual effects of stellar rotation are difficult to fully model and remove from this dataset, highlighting the difficulties to disentangle small planetary signals and photospheric noise, particularly when the orbital periods are close to the rotation period of the star. This study serves as an excellent test case for future works that aim to detect small planets orbiting `Sun-like' stars using radial velocity measurements.Comment: 16 pages, 10 figures, 13 tables, accepted for publication in A

A novel estrus-synchronization program for anestrous and cycling, suckled, beef cows

We used four herds at three Kansas ranches to evaluate the potential of two new estrus synchronization strategies to increase estrus expression and fertility of 911 crossbred suckled beef cows. The treatments included: 1) 100 μg of GnRH and a 6-mg norgestomet ear implant on day -7 and 25 mg of PG F2" and implant removal on day 0 (GnRH+NORG+PG F2"); 2) 100 μg of GnRH on day - 7 and 25 mg of PGF 2" on day 0 (GnRH+PG F2"); and 3 ) (control) 25- mg injections of PG F2" on days -14 and 0; (2×PGF2" control) . The GnRH+NORG+ PGF 2" and GnRH+PGF treatments increased (P<.01) 2" the overall percentages of cows detected in estrus by 49% and 27% and pregnancy rates by 46% and 37%, respectively, over the control group, without altering conception rate. Both treatments increase d the estrus, conception, and pregnancy rates of noncycling cows, compared to controls

A Six-Planet System Around the Star HD 34445

We present a new precision radial velocity dataset that reveals a multi-planet system orbiting the G0V star HD 34445. Our 18-year span consists of 333 precision radial velocity observations, 56 of which were previously published, and 277 which are new data from Keck Observatory, Magellan at Las Campanas Observatory, and the Automated Planet Finder at Lick Observatory. These data indicate the presence of six planet candidates in Keplerian motion about the host star with periods of 1057, 215, 118, 49, 677, and 5700 days, and minimum masses of 0.63, 0.17, 0.1, 0.05, 0.12 and 0.38 Jupiter masses respectively. The HD 34445 planetary system, with its high degree of multiplicity, its long orbital periods, and its induced stellar radial velocity half-amplitudes in the range $2 \,{\rm m\, s^{-1}} \lesssim K \lesssim 5\,{\rm m\, s^{-1}}$ is fundamentally unlike either our own solar system (in which only Jupiter and Saturn induce significant reflex velocities for the Sun), or the Kepler multiple-transiting systems (which tend to have much more compact orbital configurations)Comment: 10 pages, 11 figure

Site of semen deposition and fertility in lactating beef cows synchronized with GnRH and PGF2α

Our objective was to determine the effect of site of semen deposition on pregnancy rate in beef cows inseminated at a fixed time or after observed estrus. Cows were synchronized with a combination of gonadotropin-releasing hormone (GnRH) prostaglandin-F α (PGF). GnRH was injected 7 days before PGF (day 0; first of breeding season). The trial was conducted at two locations, one in Kansas (147 cows) and one in Colorado (313 cows). At each location, cows were assigned to be inseminated after observed estrus (ESTRUS-AI) or at a fixed time (TIMED-AI). Within these two groups, cows either were inseminated in the uterine body (BODY-bred) or in both uterine horns (HORN-bred). Cows in the ESTRUS-AI group were observed for estrus each morning and evening until day 5 afterPGF and then inseminated 12 hr after first detected estrus. Cows in the TIMED-AI group received a second dose of GnRH on day 2 and were inseminated at that time (48 to 56 hr after PGF). Heat response, AI conception rate, and pregnancy rate were analyzed for BODY-bred and HORN-bred cows within each treatment at each location. No differences in these variables occurred between locations, so the results were combined. Within the ESTRUS-AI group, neither conception rate (70% vs. 73%) nor pregnancy rate (39% vs. 40%) was different between BODY-bred and HORN-bred cows respectively. Pregnancy rate within the TIMED-AI group tended (P=.09) to be greater for BODY-bred (53%) compared to HORN-bred (42%) cows. When BODY-bred and HORN-bred treatments were combined, the pregnancy rate of TIMED-AI cows (48%) tended (P=.07) to be greater than that of ESTRUS-AI cows (39%). Timed-insemination resulted in a greater pregnancy rate than inseminating cows according to estrus. No advantage was seen in conception rates when semen was deposited in the uterine horns compared to the uterine body