5 research outputs found
Generation of Tumor-associated Cytotoxic T Lymphocytes Requires Interleukin 4 from CD8+ T Cells
<i>In Vivo</i> Coating of Bacterial Magnetic Nanoparticles by Magnetosome Expression of Spider Silk-Inspired Peptides
Magnetosomes are
natural magnetic nanoparticles with exceptional
properties that are synthesized in magnetotactic bacteria by a highly
regulated biomineralization process. Their usability in many applications
could be further improved by encapsulation in biocompatible polymers.
In this study, we explored the production of spider silk-inspired
peptides on magnetosomes of the alphaproteobacterium Magnetospirillum gryphiswaldense. Genetic fusion
of different silk sequence-like variants to abundant magnetosome membrane
proteins enhanced magnetite biomineralization and caused the formation
of a proteinaceous capsule, which increased the colloidal stability
of isolated particles. Furthermore, we show that spider silk peptides
fused to a magnetosome membrane protein can be used as seeds for silk
fibril growth on the magnetosome surface. In summary, we demonstrate
that the combination of two different biogenic materials generates
a genetically encoded hybrid composite with engineerable new properties
and enhanced potential for various applications
<i>In Vivo</i> Coating of Bacterial Magnetic Nanoparticles by Magnetosome Expression of Spider Silk-Inspired Peptides
Magnetosomes are
natural magnetic nanoparticles with exceptional
properties that are synthesized in magnetotactic bacteria by a highly
regulated biomineralization process. Their usability in many applications
could be further improved by encapsulation in biocompatible polymers.
In this study, we explored the production of spider silk-inspired
peptides on magnetosomes of the alphaproteobacterium Magnetospirillum gryphiswaldense. Genetic fusion
of different silk sequence-like variants to abundant magnetosome membrane
proteins enhanced magnetite biomineralization and caused the formation
of a proteinaceous capsule, which increased the colloidal stability
of isolated particles. Furthermore, we show that spider silk peptides
fused to a magnetosome membrane protein can be used as seeds for silk
fibril growth on the magnetosome surface. In summary, we demonstrate
that the combination of two different biogenic materials generates
a genetically encoded hybrid composite with engineerable new properties
and enhanced potential for various applications