Nurturing Nature During the Golden Age of Piracy

The impact of the natural world on an infamous era of maritime history, the Golden Age of Piracy, is immense, yet often overlooked. Piracy at the time was exacerbated by the dichotomy between rich and poor, where pirates fought for a life without the pressures of European Colonial powers. The New World was ripe for the picking, and pirates used any means possible to increase their wealth. However, geography, weather, disease, and natural disasters have all altered the historical course of piratical endeavors. This essay provides a detailed account of events where pirates were either hindered by, or benefitted from, the natural world, while struggling to understand its mechanisms. Included are stories of William Dampier, who simultaneously revolutionized the understanding of wind, while performing serious acts of piracy; of Francios L\u27Olonnais, who used the surrounding geography to succeed in his violent raids of plunder in Maracaibo; and of Henry Jennings, who capitalized on a hurricane-ravaged Spanish Treasure Fleet. Together, these stories demonstrate that pirates, like all humans, are ultimately subjects of nature

Numerical investigation of high-pressure combustion in rocket engines using Flamelet/Progress-variable models

The present paper deals with the numerical study of high pressure LOx/H2 or LOx/hydrocarbon combustion for propulsion systems. The present research effort is driven by the continued interest in achieving low cost, reliable access to space and more recently, by the renewed interest in hypersonic transportation systems capable of reducing time-to-destination. Moreover, combustion at high pressure has been assumed as a key issue to achieve better propulsive performance and lower environmental impact, as long as the replacement of hydrogen with a hydrocarbon, to reduce the costs related to ground operations and increase flexibility. The current work provides a model for the numerical simulation of high- pressure turbulent combustion employing detailed chemistry description, embedded in a RANS equations solver with a Low Reynolds number k-omega turbulence model. The model used to study such a combustion phenomenon is an extension of the standard flamelet-progress-variable (FPV) turbulent combustion model combined with a Reynolds Averaged Navier-Stokes equation Solver (RANS). In the FPV model, all of the thermo-chemical quantities are evaluated by evolving the mixture fraction Z and a progress variable C. When using a turbulence model in conjunction with FPV model, a probability density function (PDF) is required to evaluate statistical averages of chemical quantities. The choice of such PDF must be a compromise between computational costs and accuracy level. State- of-the-art FPV models are built presuming the functional shape of the joint PDF of Z and C in order to evaluate Favre-averages of thermodynamic quantities. The model here proposed evaluates the most probable joint distribution of Z and C without any assumption on their behavior.Comment: presented at AIAA Scitech 201

Efficiently Enumerating Hitting Sets of Hypergraphs Arising in Data Profiling

We devise an enumeration method for inclusion-wise minimal hitting sets in hypergraphs. It has delay O(mk* +1 · n2) and uses linear space. Hereby, n is the number of vertices, m the number of hyperedges, and k* the rank of the transversal hypergraph. In particular, on classes of hypergraphs for which the cardinality k* of the largest minimal hitting set is bounded, the delay is polynomial. The algorithm solves the extension problem for minimal hitting sets as a subroutine. We show that the extension problem is W[3]-complete when parameterised by the cardinality of the set which is to be extended. For the subroutine, we give an algorithm that is optimal under the exponential time hypothesis. Despite these lower bounds, we provide empirical evidence showing that the enumeration outperforms the theoretical worst-case guarantee on hypergraphs arising in the profiling of relational databases, namely, in the detection of unique column combinations

Spiropyran Photoisomerization Dynamics in Multiresponsive Hydrogels

Light-responsive, spiropyran-functionalized hydrogels have been used to create reversibly photoactuated structures for applications ranging from microfluidics to nonlinear optics. Tailoring a spiropyran-functionalized hydrogel system for a particular application requires an understanding of how co-monomer composition affects the switching dynamics of the spiropyran chromophore. Such gels are frequently designed to be responsive to different stimuli such as light, temperature, and pH. The coupling of these influences can significantly alter spiropyran behavior in ways not currently well understood. To better understand the influence of responsive co-monomers on the spiropyran isomerization dynamics, we use UV-vis spectroscopy and time-dependent fluorescence intensity measurements to study spiropyran-modified hydrogels polymerized from four common hydrogel precursors of different pH and temperature responsivity: acrylamide, acrylic acid, N-isopropylacrylamide, and 2-(dimethylamino)ethyl methacrylate. In acidic and neutral gels, we observe unusual nonmonotonic, triexponential fluorescence dynamics under 405 nm irradiation that cannot be explicated by either the established spiropyran-merocyanine interconversion model or hydrolysis. To explain these results, we introduce an analytical model of spiropyran interconversions that includes H-aggregated merocyanine and its light-triggered disaggregation under 405 nm irradiation. This model provides an excellent fit to the observed fluorescence dynamics and elucidates exactly how creating an acidic internal gel environment promotes the fast and complete conversion of the hydrophilic merocyanine speciesto the hydrophobic spiropyran form, which is desired in most light-sensitive hydrogel actuators. This can be achieved by incorporating acrylic acid monomers and by minimizing the aggregate concentration. Beyond spiropyran-functionalized gel actuators, these conclusions are particularly critical for nonlinear optical computing applications

Activities of the Boom and Chassis Group

Group One of the NASA Lunar Enabler Project has designed the primary chassis and boom structures for the lunar vehicle. Both components also feature V-clamps that were adapted to interface connections within the structure. The chassis features a front end, rear end section, middle cross-section, and face plate. The rear section contains an extra compartment for the engine, hydraulic pump, fuel bottles, and oil reservoir necessary for the wheel drives. Each section consists of tubular aluminum 6061-T6. The boom features four degrees of freedom system, where the minimum factor of safety of any part is 1.5 (but, normally much higher). It consists of a tapered upper boom, lower boom, and three elbows that complement the articulation joints. Each section of the boom has been constructed from aluminum 6061-T6. There are four joints and eight V-clamps in the boom assembly. The V-clamps feature support rings that prevent axial rotation. They provide easy adaptability and assembly

A Vibrio cholerae Classical TcpA Amino Acid Sequence Induces Protective Antibody That Binds an Area Hypothesized To Be Important for Toxin-Coregulated Pilus Structure

Vibrio cholerae is a gram-negative bacterium that has been associated with cholera pandemics since the early 1800s. Whole-cell, killed, and live-attenuated oral cholera vaccines are in use. We and others have focused on the development of a subunit cholera vaccine that features standardized epitopes from various V. cholerae macromolecules that are known to induce protective antibody responses. TcpA protein is assembled into toxin-coregulated pilus (TCP), a type IVb pilus required for V. cholerae colonization, and thus is a strong candidate for a cholera subunit vaccine. Polypeptides (24 to 26 amino acids) in TcpA that can induce protective antibody responses have been reported, but further characterization of their amino acid targets relative to tertiary or quaternary TCP structures has not been done. We report a refinement of the TcpA sequences that can induce protective antibody. One sequence, TcpA 15 (residues 170 to 183), induces antibodies that bind linear TcpA in a Western blot as well as weakly bind soluble TcpA in solution. These antibodies bind assembled pili at high density and provide 80 to 100% protection in the infant mouse protection assay. This is in sharp contrast to other anti-TcpA peptide sera (TcpA 11, TcpA 13, and TcpA 17) that bind very strongly in Western blot and solution assays yet do not provide protection or effectively bind TCP, as evidenced by immunoelectron microscopy. The sequences of TcpA 15 that induce protective antibody were localized on a model of assembled TCP. These sequences are centered on a site that is predicted to be important for TCP structure

Dietary and physical activity strategies to prevent type 2 diabetes in South Asian adults:protocol for a systematic review

Type 2 diabetes (T2D) is a major health concern among populations of South Asian ethnicity. Although dietary and physical activity interventions may reduce the risk of T2D, the effectiveness has been moderate among South Asians. This might (in part) be because this subgroup follows strategies that were originally developed for interventions among other populations. Therefore, this review aims to assess the evidence for the current dietary and physical activity strategies recommended in T2D prevention intervention studies and guidelines for South Asians. Included will be all studies and guidelines on dietary and/or physical activity strategies to prevent T2D in adult South Asians. Two reviewers will search online databases from their start until the present date for published and unpublished experimental/quasiexperimental studies, with at least an abstract in English. References of identified articles and key reviews will be screened for additional studies. Guidelines will be identified by searches in online databases and websites of public organisations. Finally, expert consultations will be held to supplement any missing information. Trial quality will be assessed with the Quality Assessment Tool for Quantitative Studies Data, and guidelines with the Appraisal of Guidelines for Research & Evaluation II. Data on the strategies recommended, targeting and evidence on effectiveness will be extracted by two reviewers and presented in tabular and narrative forms. Recommendations will be compared with the National Institute for Health and Care Excellence guidelines [PH35]. Overall findings on dietary and physical activity recommendations, as well as findings for specific subgroups (eg, by sex), will be discussed. Ethics assessment is not required. Start date: 1 January 2016, finishing and reporting date 31 July 2016. Results will be published in a peer-reviewed scientific journal, the project report of EuroDHYAN (www.eurodhyan.eu) and in a PhD dissertation. The protocol is registered with the International Prospective Register of Systematic Reviews (PROSPERO) registration number CRD42015027067

Roles for a Lipid Phosphatase in the Activation of its Opposing Lipid Kinase

Fig4 is a phosphoinositide phosphatase that converts PI3,5P2 to PI3P. Paradoxically, mutation of Fig4 results in lower PI3,5P2, indicating that Fig4 is also required for PI3,5P2 production. Fig4 promotes elevation of PI3,5P2, in part, through stabilization of a protein complex that includes its opposing lipid kinase, Fab1, and the scaffold protein Vac14. Here we show that multiple regions of Fig4 contribute to its roles in the elevation of PI3,5P2: Its catalytic site, an N-terminal disease-related surface, and a C-terminal region. We show that mutation of the Fig4 catalytic site enhances the formation of the Fab1-Vac14-Fig4 complex, and reduces the ability to elevate PI3,5P2. This suggests that independent of its lipid phosphatase function, the active site plays a role in the Fab1-Vac14-Fig4 complex. We also show that the N-terminal disease-related surface contributes to the elevation of PI3,5P2 and promotes Fig4 association with Vac14 in a manner that requires the Fig4 C-terminus. We find that the Fig4 C-terminus alone interacts with Vac14 in vivo and retains some functions of full-length Fig4. Thus, a subset of Fig4 functions are independent of its phosphatase domain and at least three regions of Fig4 play roles in the function of the Fab1-Vac14-Fig4 complex