Analisis Faktor-Faktor yang Memengaruhi Tingkat Kepatuhan Wajib Pajak Orang Pribadi di Lingkungan Kantor Pelayanan Pajak Pratama, Tigaraksa Tangerang

Tax collection is not an easy matter. Active participation from the tax authorities also requires the willingness of the taxpayer. A public reaction can be seen from the taxpayer\u27s willingness to pay taxes. Willingness and awareness to pay taxes represent a value contributed by someone (which has been determined by regulation). Tax is used to finance public expenditures without any direct benefit. Taxpayer\u27s awareness about taxation functions as state funding is needed to improve tax compliance and to determine the level of tax compliance in implementing their tax obligations. Limitation of the scope of this study is the effect of the level of awareness of paying taxes, taxpayer\u27s understanding about tax benefits, tax penalties, and understanding of service quality to the tax authorities of individual taxpayer compliance in the fulfillment of tax obligations, as well as restricted to data obtained through questionnaires received and filled by the individual taxpayer of Tigaraksa Pratama Tax Office area. Data were obtained through questionnaire and processed and analyzed using parametric statistical tests and multiple linear regression with 4 independent variables and one dependent variable resulted in the conclusion that the factors that most influence taxpayer compliance in carrying out its tax liability is the use of sanctions against taxpayers who do not carry out its obligations under applicable legislation

Structure of linker molecules employed for biotin attachment to the stem–loop structures

<p><b>Copyright information:</b></p><p>Taken from "Immobilized stem–loop structured probes as conformational switches for enzymatic detection of microbial 16S rRNA"</p><p>Nucleic Acids Research 2005;33(11):e101-e101.</p><p>Published online 24 Jun 2005</p><p>PMCID:PMC1159122.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> ARP-biotin [-(aminooxyacetyl)--(-biotinoyl)hydrazine] is attached via Schiff base chemistry to an aldehyde group generated by oxidative cleavage of the terminal ribose moiety of cytidin (rC). C3-Biotin contains a C3-ether-glycerol linker attached to the biotin residue via an amide bond. In TEG-biotin, the C3-ether-glycerol linker is extended by a triethyleneglycol (TEG) moiety

Titration of PsVs by transduction of HEK293TT cells.

<p>(A) HEK293TT cells were transduced with the indicated PsVs carrying pEGFP as reporter plasmid. 72h after transduction, GFP-positive cells were counted and titer was calculated as transducing units per ml PsVs. Each data point represents one PsV preparation. (B) To compare the amount of transducing units with the amount of particles carrying the pEGFP reporter plasmid, plasmid DNA was isolated from PsVs and quantified by qPCR to obtain the pEGFP copy number in the sample.</p

Effect of ι-carrageenan on transduction with PcPV1 and MfPV11.

<p>(A) Different doses of ι-carrageenan were added to the cell culture medium immediately before transduction of HEK293TT cells with PcPV1 PsVs carrying a G.Luc reporter plasmid. Luciferase assay was performed 72h after transduction. Shown are mean values and standard deviation of three independent PcPV1 PsV preparations. Statistical analysis was performed by 2way ANOVA and Tukey’s multiple comparison test. (B) Additionally, HEK293TT cells were transduced with PcPV1 and MfPV11 PsVs carrying a pEGFP reporter plasmid with (+) and without (-) addition of 10μg/ml ι-carrageenan. GFP-positive cells were counted 72h after transduction and transducing units were calculated. Each data point represents one experiment with one PsV preparation. Dashed line indicates limit of detection. Statistical analysis was performed using t-test.</p

Bioluminescence imaging after intramuscular application.

<p>50μl of the indicated PsV preparation were injected into the left thigh muscle. Bioluminescent imaging was performed approx. 3h after injection (day 0) to test for any free F.Luc and subsequently in a weekly manner.</p

Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps-5

<p><b>Copyright information:</b></p><p>Taken from "Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps"</p><p>http://www.virologyj.com/content/4/1/51</p><p>Virology Journal 2007;4():51-51.</p><p>Published online 5 Jun 2007</p><p>PMCID:PMC1892776.</p><p></p>cate mutated consensus poly(A) signals. B) Western blot analysis of RSV-F protein levels after transfection of the indicated expression plasmids. An expression plasmid for EGFP was cotransfected. Undiluted lysates had equal protein content and a similar amount of fluorescent activity revealing constant transfection efficiency. Numbers above the lanes indicate the dilution at which the cell lysates were loaded on the gel

Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps-2

<p><b>Copyright information:</b></p><p>Taken from "Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps"</p><p>http://www.virologyj.com/content/4/1/51</p><p>Virology Journal 2007;4():51-51.</p><p>Published online 5 Jun 2007</p><p>PMCID:PMC1892776.</p><p></p> of primers used for the PCR analyses. The scale indicates the distance to the transcriptional start site. AATAAA: consensus signal for polyadenylation. B) Characterisation of splicing. 293T cells were transfected with pIF. Cytoplasmic RNA was isolated from transfected cells and reverse transcribed by oligo-dT priming (pIFcDNA). A PCR spanning the splice sites was performed with primers: 5'UTR-s and RSV-F-ia. The size of the PCR-products was compared to the size obtained in parallel PCR using pIΔIFand pIFplasmid-DNA (pDNA) as templates. HO: negative control. C) Characterisation of poly(A) signal usage. 293T cells were transfected with the indicated plasmids. Cytoplasmic RNA was isolated from transfected cells and reverse transcribed by priming with Oligo(dT)Add-a. As a control for DNA contamination, the reverse transcription reaction was also performed without the enzyme (-). The cDNA was amplified by PCR with primers 5' UTR-s and Oligo(dT)Add-a and the size of the PCR products was determined by agarose gel electrophoresis. D) PCR products from pIFtransfected cells were cloned and sequenced. The 3' end of the sequence obtained in 9 of 10 clones (RSV-F mRNA exp.) is shown aligned to the RSV-F sequence of the parental plasmid (RSV-F mRNA theor.)

Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps-4

<p><b>Copyright information:</b></p><p>Taken from "Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps"</p><p>http://www.virologyj.com/content/4/1/51</p><p>Virology Journal 2007;4():51-51.</p><p>Published online 5 Jun 2007</p><p>PMCID:PMC1892776.</p><p></p>taining an inframe 3'-terminal myc-tag. Similar transfection efficiencies were controlled by measuring the luciferase activity in cell supernatants (not shown). Six hours following transfection cells were infected with the GFP expressing recombinant RSV at an MOI of 2 (+). As negative control, cells were also left uninfected (-). GFP expression analysis revealed similar infection efficiencies (data not shown). Equal amounts of protein were analysed in non-reducing Western blot analysis using a monoclonal antibody to the myc-tag 48 h after transfection

Transmission electron microscopy of HPV16, PcPV1 and MfPV11 VLPs.

<p>Purified VLPs were fixed for 24h at room temperature with formaldehyde, contrasted with phosphotungstic acid and analyzed by transmission electron microscopy.</p

Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps-0

<p><b>Copyright information:</b></p><p>Taken from "Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps"</p><p>http://www.virologyj.com/content/4/1/51</p><p>Virology Journal 2007;4():51-51.</p><p>Published online 5 Jun 2007</p><p>PMCID:PMC1892776.</p><p></p>ked by the human cytomegalovirus immediate early promoter/enhancer region (CMV) and the bovine growth hormone poly(A) signal (pA). Angled black arrows mark the transcriptional start point. The pIGvector contains intron A and flanking untranslated exonic regions E1 and E2 of the cytomegalovirus immediate early gene. In pIΔIGthe exon boundaries were precisely fused by deleting the intron. B) Northern blot analysis. Cells were cotransfected with the indicated VSV-G expression plasmids, a codon optimised HIV-1 expression plasmid (Hgp) and the lentiviral vector construct VICGΔBH containing a GFP expression cassette. Poly(A) RNA was isolated from transfected cells and analysed by Northern blot with a probe spanning the transcribed region of the BGH poly(A) signal present on all VSV-G transcripts and the positive 5 kb HIV-1 transcript. C) Western blot analyses. Cells were cotransfected with the indicated VSV-G expression plasmids, an SIV expression plasmid (SgpΔ2) and the lentiviral vector construct VICGΔBH containing a GFP expression cassette. Monoclonal antibodies to HIV-1 p24 capsid protein, which is cross reactive to SIV p27, or to VSV-G, respectively, were used for detection of the viral proteins in lysates of transfected cells