<p><b>Copyright information:</b></p><p>Taken from "Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps"</p><p>http://www.virologyj.com/content/4/1/51</p><p>Virology Journal 2007;4():51-51.</p><p>Published online 5 Jun 2007</p><p>PMCID:PMC1892776.</p><p></p>taining an inframe 3'-terminal myc-tag. Similar transfection efficiencies were controlled by measuring the luciferase activity in cell supernatants (not shown). Six hours following transfection cells were infected with the GFP expressing recombinant RSV at an MOI of 2 (+). As negative control, cells were also left uninfected (-). GFP expression analysis revealed similar infection efficiencies (data not shown). Equal amounts of protein were analysed in non-reducing Western blot analysis using a monoclonal antibody to the myc-tag 48 h after transfection
