<p><b>Copyright information:</b></p><p>Taken from "Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps"</p><p>http://www.virologyj.com/content/4/1/51</p><p>Virology Journal 2007;4():51-51.</p><p>Published online 5 Jun 2007</p><p>PMCID:PMC1892776.</p><p></p>ked by the human cytomegalovirus immediate early promoter/enhancer region (CMV) and the bovine growth hormone poly(A) signal (pA). Angled black arrows mark the transcriptional start point. The pIGvector contains intron A and flanking untranslated exonic regions E1 and E2 of the cytomegalovirus immediate early gene. In pIΔIGthe exon boundaries were precisely fused by deleting the intron. B) Northern blot analysis. Cells were cotransfected with the indicated VSV-G expression plasmids, a codon optimised HIV-1 expression plasmid (Hgp) and the lentiviral vector construct VICGΔBH containing a GFP expression cassette. Poly(A) RNA was isolated from transfected cells and analysed by Northern blot with a probe spanning the transcribed region of the BGH poly(A) signal present on all VSV-G transcripts and the positive 5 kb HIV-1 transcript. C) Western blot analyses. Cells were cotransfected with the indicated VSV-G expression plasmids, an SIV expression plasmid (SgpΔ2) and the lentiviral vector construct VICGΔBH containing a GFP expression cassette. Monoclonal antibodies to HIV-1 p24 capsid protein, which is cross reactive to SIV p27, or to VSV-G, respectively, were used for detection of the viral proteins in lysates of transfected cells
