A Synoptic Climatology of Derecho Producing Mesoscale Convective Systems in the North-Central Plains

Synoptic-scale environments favourable for producing derechos, or widespread convectively induced windstorms, in the North-Central Plains are examined with the goal of providing pattern-recognition:diagnosis techniques. Fifteen derechos were identified across the North-Central Plains region during 1986–1995. The synoptic environment at the initiation, mid-point and decay of each derecho was then evaluated using surface, upper-air and National Center for Atmospheric Research (NCAR):National Center for Environmental Prediction (NCEP) reanalysis datasets. Results suggest that the synoptic environment is critical in maintaining derecho producing mesoscale convective systems (DMCSs). The synoptic environment in place downstream of the MCS initiation region determines the movement and potential strength of the system. Circulation around surface low pressure increased the instability gradient and maximized leading edge convergence in the initiation region of nearly all events regardless of DMCS location or movement. Other commonalities in the environments of these events include the presence of a weak thermal boundary, high convective instability and a layer of dry low-to-mid-tropospheric air. Of the two corridors sampled, northeastward moving derechos tend to initiate east of synoptic-scale troughs, while southeastward moving derechos form on the northeast periphery of a synoptic-scale ridge. Other differences between these two DMCS events are also discussed

Mycobacterium abscessus: Shapeshifter of the Mycobacterial World

In this review we will focus on unique aspects of Mycobacterium abscessus (MABS) which we feel earn it the designation of “shapeshifter of the mycobacterial world.” We will review its emergence as a distinct species, the recognition and description of MABS subspecies which are only now being clearly defined in terms of pathogenicity, its ability to exist in different forms favoring a saprophytic lifestyle or one more suitable to invasion of mammalian hosts, as well as current challenges in terms of antimicrobial therapy and future directions for research. One can see in the various phases of MABS, a species transitioning from a free living saprophyte to a host-adapted pathogen

Trehalose polyphleates, external cell wall lipids in mycobacterium abscessus, are associated with the formation of clumps with cording morphology, which have been associated with virulence

Mycobacterium abscessus is a reemerging pathogen that causes pulmonary diseases similar to tuberculosis, which is caused by Mycobacterium tuberculosis. When grown in agar medium, M. abscessus strains generate rough (R) or smooth colonies (S). R morphotypes are more virulent than S morphotypes. In searching for the virulence factors responsible for this difference, R morphotypes have been found to form large aggregates (clumps) that, after being phagocytozed, result in macrophage death. Furthermore, the aggregates released to the extracellular space by damaged macrophages grow, forming unphagocytosable structures that resemble cords. In contrast, bacilli of the S morphotype, which do not form aggregates, do not damage macrophages after phagocytosis and do not form cords. Cording has also been related to the virulence of M. tuberculosis. In this species, the presence of mycolic acids and surface-exposed cell wall lipids has been correlated with the formation of cords. The objective of this work was to study the roles of the surface-exposed cell wall lipids and mycolic acids in the formation of cords in M. abscessus. A comparative study of the pattern and structure of mycolic acids was performed on R (cording) and S (non-cording) morphotypes derived from the same parent strains, and no differences were observed between morphotypes. Furthermore, cords formed by R morphotypes were disrupted with petroleum ether (PE), and the extracted lipids were analyzed by thin layer chromatography, nuclear magnetic resonance spectroscopy and mass spectrometry. Substantial amounts of trehalose polyphleates (TPP) were recovered as major lipids from PE extracts, and images obtained by transmission electron microscopy suggested that these lipids are localized to the external surfaces of cords and R bacilli. The structure of M. abscessus TPP was revealed to be similar to those previously described in Mycobacterium smegmatis. Although the exact role of TPP is unknown, our results demonstrated that TPP are not toxic by themselves and have a function in the formation of clumps and cords in M. abscessus, thus playing an important role in the pathogenesis of this species

Mycobacteria clumping increase their capacity to damage macrophages

The rough morphotypes of non-tuberculous mycobacteria have been associated with the most severe illnesses in humans. This idea is consistent with the fact that Mycobacterium tuberculosis presents a stable rough morphotype. Unlike smooth morphotypes, the bacilli of rough morphotypes grow close together, leaving no spaces among them and forming large aggregates (clumps). Currently, the initial interaction of macrophages with clumps remains unclear. Thus, we infected J774 macrophages with bacterial suspensions of rough morphotypes of M. abscessus containing clumps and suspensions of smooth morphotypes, primarily containing isolated bacilli. Using confocal laser scanning microscopy and electron microscopy, we observed clumps of at least five rough-morphotype bacilli inside the phagocytic vesicles of macrophages at 3 h post-infection. These clumps grew within the phagocytic vesicles, killing 100% of the macrophages at 72 h post-infection, whereas the proliferation of macrophages infected with smooth morphotypes remained unaltered at 96 h post-infection. Thus, macrophages phagocytose large clumps, exceeding the bactericidal capacities of these cells. Furthermore, proinflammatory cytokines and granuloma-like structures were only produced by macrophages infected with rough morphotypes. Thus, the present study provides a foundation for further studies that consider mycobacterial clumps as virulence factors

Discordance between imaging and immunohistochemistry in unilateral primary aldosteronism

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/139975/1/cen13442.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/139975/2/cen13442_am.pd

Star-forming galaxies in low-redshift clusters: Data and integrated galaxy properties

This paper is a continuation of an ongoing study of the evolutionary processes affecting cluster galaxies. Both CCD R band and H alpha narrow-band imaging was used to determine photometric parameters (m_(r), r_(24), H alpha flux and equivalent width) and derive star formation rates for 227 CGCG galaxies in 8 low-redshift clusters. The galaxy sample is a subset of CGCG galaxies in an objective prism survey of cluster galaxies for H alpha emission. It is found that detection of emission-line galaxies in the OPS is 85%, 70%, and 50% complete at the mean surface brightness values of 1.25 x 10^(-19), 5.19 x 10^(-20), and 1.76 x 10^(-20) W m^(-2) arcsec^(-2), respectively, measured within the R band isophote of 24 mag arcsec^(-2) for the galaxy. The CCD data, together with matched data from a recent H alpha galaxy survey of UGC galaxies within 3000 km s^(-1), will be used for a comparative study of R band and H alpha surface photometry between cluster and field spirals.Comment: Accepted for publication in A&A. 11 pages, including 6 figure

Laboratory tests of IEC DER object models for grid applications.

This report describes a Cooperative Research and Development Agreement (CRADA) between Salt River Project Agricultural Improvement and Power District (SRP) and Sandia National Laboratories to jointly develop advanced methods of controlling distributed energy resources (DERs) that may be located within SRP distribution systems. The controls must provide a standardized interface to allow plug-and-play capability and should allow utilities to take advantage of advanced capabilities of DERs to provide a value beyond offsetting load power. To do this, Sandia and SRP field-tested the IEC 61850-7-420 DER object model (OM) in a grid environment, with the goal of validating whether the model is robust enough to be used in common utility applications. The diesel generator OM tested was successfully used to accomplish basic genset control and monitoring. However, as presently constituted it does not enable plug-and-play functionality. Suggestions are made of aspects of the standard that need further development and testing. These problems are far from insurmountable and do not imply anything fundamentally unsound or unworkable in the standard

Star-forming galaxies in low-redshift clusters: Comparison of integrated properties of cluster and field galaxies

We investigate the effect of the cluster environment on the star formation properties of galaxies in 8 nearby Abell clusters. Star formation properties are determined for individual galaxies using the equivalent width of H alpha plus [NII] line emission from narrow-band imaging. Equivalent width distributions are derived for each galaxy type in each of 3 environments - cluster, supercluster (outside the cluster virial radius) and field. The effects of morphological disturbance on star formation are also investigated. We identify a population of early-type disk galaxies in the cluster population with enhanced star formation compared to their field counterparts. The enhanced cluster galaxies frequently show evidence of disturbance, and the disturbed galaxies show marginal evidence for a higher velocity dispersion, possibly indicative of an infalling population.Comment: 9 pages, 10 figures, accepted for publication in Astronomy and Astrophysic