Historical Analysis of Submerged Aquatic Vegetation (SAV) in the Potomac River and Analysis of Bay-wide SAV Data to Establish a New Acreage Goal : Final Report

Throughout most regions of the Chesapeake Bay and its tributaries both direct and anecdotal evidence has indicated that large-scale declines of submerged aquatic vegetation (SAV) occurred in the late 1960s and early 1970s (Orth and Moore 1983). These declines have been related to increasing amounts of non-point inputs of nutrients and sediments in the bay system resulting from development of the bay’s shorelines and watershed (Twilley et al. 1985). Currently there are approximately 89,659 acres of SAV in Chesapeake Bay (Orth et al. 2003). Although it has been estimated that this is approximately 15% of the bay’s historical SAV distribution, most comprehensive analyses have been based on 1971 or later aerial photography and the distributions of SAV prior to this time in many regions are not well known. SAV is a highly valuable resource and its presence serves as an important indicator of local water quality conditions (Dennison et al. 1993). SAV growth and survival can be decreased by high levels of turbidity and nutrient enrichment. Because SAV beds are non-motile, their presence serves as an integrating measure of variable water quality conditions in local areas (Moore et al. 1996). Because of the direct relationships between SAV and water quality, trends in the distribution and abundance of SAV over time are also very useful in understanding trends in water quality. Review of photographic evidence from a number of sites dating back to 1937 suggests that SAV, once abundant throughout the Chesapeake Bay system, have declined from historical levels and therefore water quality conditions may have similarly deteriorated (Orth and Moore 1983)

Optical spectroscopy for real-time neuronavigation during deep brain stimulation surgeries

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Genetic diversity of CHC22 clathrin impacts its function in glucose metabolism

CHC22 clathrin plays a key role in intracellular membrane traffic of the insulin-responsive glucose transporter GLUT4 in humans. We performed population genetic and phylogenetic analyses of the CHC22-encoding CLTCL1 gene, revealing independent gene loss in at least two vertebrate lineages, after arising from gene duplication. All vertebrates retained the paralogous CLTC gene encoding CHC17 clathrin, which mediates endocytosis. For vertebrates retaining CLTCL1, strong evidence for purifying selection supports CHC22 functionality. All human populations maintained two high frequency CLTCL1 allelic variants, encoding either methionine or valine at position 1316. Functional studies indicated that CHC22-V1316, which is more frequent in farming populations than in hunter-gatherers, has different cellular dynamics than M1316-CHC22 and is less effective at controlling GLUT4 membrane traffic, altering its insulin-regulated response. These analyses suggest that ancestral human dietary change influenced selection of allotypes that affect CHC22’s role in metabolism and have potential to differentially influence the human insulin response

United States Department of State Diplomacy Laboratory An External Evaluation of Kosovo’s American Corners Program

This report provides analysis, findings, and recommendations as they pertain to American Corners in Kosovo as a contribution to an ongoing U.S. Department of State program. Stemming from America’s foreign policy, three corners located throughout Kosovo have been working to educate the populations they serve and to unite the country and improve its identity. The Corners, Pristina, Prizren, and Mitrovica serve their communities with a variety of educational and informational topics to include law, economics, language, STEM, and the promotion of regional stability with economic growth. It is the goal of the research to improve the Corners and support Kosovo as it works to become a democratic state. Methods of research derived from a variety of resources; open online resources, the American Corner’s website, lectures, and resources from the Indiana‐Purdue University Library database. Research was also conducted by Zoom meetings with the American Corner’s staff members. Staff members were asked specific, structured questions, and were also given open-ended discussion opportunities. Findings from research show an overwhelming response for improved collaboration among the three Corners. If done together program planning and implementation would benefit. For further collaboration, there is a need for increased involvement with the US Embassy to implement more cohesive training for all Corners staff. Some staff and space requirements and subject recommendations are presented as well. The authors recognize the limitations of the research. None of the authors have spent time in Kosovo first‐hand. The research is also being conducted during a serious international Pandemic, creating an environment and issues that may not always be consistent. The corners are also a relatively new foreign policy State Department enterprise in Kosovo and as well have been impacted by the Pandemic

HLA-DR Alpha 2 Mediates Negative Signalling via Binding to Tirc7 Leading to Anti-Inflammatory and Apoptotic Effects in Lymphocytes In Vitro and In Vivo

Classically, HLA-DR expressed on antigen presenting cells (APC) initiates lymphocyte activation via presentation of peptides to TCR bearing CD4+ T-Cells. Here we demonstrate that HLA-DR alpha 2 domain (sHLA-DRα2) also induces negative signals by engaging TIRC7 on lymphocytes. This interaction inhibits proliferation and induces apoptosis in CD4+ and CD8+ T-cells via activation of the intrinsic pathway. Proliferation inhibition is associated with SHP-1 recruitment by TIRC7, decreased phosphorylation of STAT4, TCR-ζ chain & ZAP70, and inhibition of IFN-γ and FasL expression. HLA-DRα2 and TIRC7 co-localize at the APC-T cell interaction site. Triggering HLA-DR - TIRC7 pathway demonstrates that sHLA-DRα2 treatment inhibits proinflammatory-inflammatory cytokine expression in APC & T cells after lipopolysaccaride (LPS) stimulation in vitro and induces apoptosis in vivo. These results suggest a novel antiproliferative role for HLA-DR mediated via TIRC7, revise the notion of an exclusive stimulatory interaction of HLA-DR with CD4+ T cells and highlights a novel physiologically relevant regulatory pathway

Structural and regulatory diversity shape HLA-C protein expression levels

Expression of HLA-C varies widely across individuals in an allele-specific manner. This variation in expression can influence efficacy of the immune response, as shown for infectious and autoimmune diseases. MicroRNA binding partially influences differential HLA-C expression, but the additional contributing factors have remained undetermined. Here we use functional and structural analyses to demonstrate that HLA-C expression is modulated not just at the RNA level, but also at the protein level. Specifically, we show that variation in exons 2 and 3, which encode the α1/α2 domains, drives differential expression of HLA-C allomorphs at the cell surface by influencing the structure of the peptide-binding cleft and the diversity of peptides bound by the HLA-C molecules. Together with a phylogenetic analysis, these results highlight the diversity and long-term balancing selection of regulatory factors that modulate HLA-C expression

Thin Shell, High Velocity Inertial Confinement Fusion Implosions on the National Ignition Facility

Experiments have recently been conducted at the National Ignition Facility utilizing inertial confinement fusion capsule ablators that are 175 and 165  μm in thickness, 10% and 15% thinner, respectively, than the nominal thickness capsule used throughout the high foot and most of the National Ignition Campaign. These three-shock, high-adiabat, high-foot implosions have demonstrated good performance, with higher velocity and better symmetry control at lower laser powers and energies than their nominal thickness ablator counterparts. Little to no hydrodynamic mix into the DT hot spot has been observed despite the higher velocities and reduced depth for possible instability feedthrough. Early results have shown good repeatability, with up to 1/2 the neutron yield coming from α-particle self-heating