Process for mineral refining

A process for refining inorganic salts of an inorganic acid which is displaceable by sulfuric acid, such as phosphate and carbonate minerals like apatite and dolomite. In the process, an aqueous reaction mixture is provided comprising the salt and an aqueous reaction medium, and sulfur dioxide and oxygen are introduced into the aqueous medium. The salt, sulfur dioxide and oxygen are reacted in the aqueous medium to release the inorganic acid and to convert the inorganic salt to the corresponding inorganic sulfate

Issues and Opinions: Assessing the Emphasis on Information Security in the Systems Analysis and Design Course

Due to several recent highly publicized information breaches, information security has gained a higher profile. Hence, it is reasonable to expect that information security would receive an equally significant emphasis in the education of future systems professionals. A variety of security standards that various entities (e.g., NIST, COSO, ISACA-COBIT, ISO) have put forth emphasize the importance of information security from the very beginning of the system development lifecycle (SDLC) to avoid significant redesign in later phases. To determine the emphasis on security in typical systems analysis and design (SA&D) courses, we examine (1) to what extent security is emphasized in the core SA&D courses and (2) at what phase in the SDLC do most SA&D courses begin to emphasize security. In order to address these questions, we reviewed SA&D textbooks currently on the market to identify how extensively they cover security-related issues. Given the fairly high awareness of information security in practice, we expected to see an equally high emphasis on such matters in the textbooks. However, our review suggests that this is not the case, which suggests a gap in our preparation. To address this gap, we offer a proposal for modifying a portion of the SA&D curricula

Isolation of Human Antigen-Specific Antibodies from Memory B-Cells Nearly Two Years Post Vaccination

Isolation and production of therapeutic human monoclonal antibodies (mAbs) traditionally utilizes a handful of techniques including antibody engineering, phage display, hybridoma generation from transgenic mice or EBV immortalization of B-cells. Over the past decade a new approach has emerged that attempts to extract antigen-specific memory B-cells from the peripheral blood of individuals vaccinated or infected with the target. Initial attempts focused on culturing B-cells and inducing differentiation to plasmablasts for analysis of antibody-antigen specificity, but results were largely mixed due to difficult culture conditions and/or rarity of target cells. With advancing technology in cell sorting, single antigen-specific memory B-cells can be identified and sorted with fluorescently labeled antigens. This method has produced virus-specific mAbs from HIV-infected patients and tetanus-specific mAbs within weeks after Tdap immunization. Many other studies claim to have found antigen-specific mAbs months to years after immunization or clearance of an infection; however, these studies fail to provide direct evidence of antibody specificity by cloning and expressing the mAbs from B-cells. Here we report the efficient isolation of tetanus-specific mAbs from a subject Td-immunized almost two years prior to blood draw. Initially, the total B-cell population was isolated from peripheral blood mononuclear cells enriched by negative selection, then stained to identify tetanus-specific memory B-cells. These cells were individually sorted and PCR was performed to amplify heavy and light chain variable regions of the B-cell’s antibody mRNA. After sequencing, 15 of 42 samples produced both heavy and light chain antibody sequence and 11 mAbs were cloned and transiently expressed. ELISA analysis indicated 5 of the 11 mAbs bound the Hc protein fragment of tetanus toxin and 3 were specific for Hc. We plan to extend this initial success to additional targets and longer gaps between vaccination and B-cell isolation to identify functional therapeutic human antibodies

EXPERIMENTAL ERROR IN AGRONOMIC FIELD TRIALS

Agronomic experiments often summarize work carried out in trials run in several locations over several years, referred to generically as environments. The appropriate statistical analyses for these experiments depend on definitions used for experimental error. The results of one such experiment, in which identical designs were used in each environment, illustrate the commonalities and differences in analyses that can result from using different definitions of experimental error

Targeted Mutagenesis of a Therapeutic Human Monoclonal IgG1 Antibody Prevents Gelation at High Concentrations

A common challenge encountered during development of high concentration monoclonal antibody formulations is preventing self-association. Depending on the antibody and its formulation, self-association can be seen as aggregation, precipitation, opalescence or phase separation. Here we report on an unusual manifestation of self-association, formation of a semi-solid gel or “gelation”. Therapeutic monoclonal antibody C4 was isolated from human B cells based on its strong potency in neutralizing bacterial toxin in animal models. The purified antibody possessed the unusual property of forming a firm, opaque white gel when it was formulated at concentrations \u3e40 mg/mL and the temperature wa