THE "GREAT DECISIONS" PROGRAM IN OREGON

Teaching/Communication/Extension/Profession,

APPROACH D: MULTIPLE PRICING

Demand and Price Analysis,

Characterization of cells of amniotic fluids by immunological identification of intermediate-sized filaments: Presence of cells of different tissue origin

Antibodies against intermediate-sized filaments, of the prekeratin or vimentin type, were used to investigate the presence of these filaments by indirect immunofluorescence microscopy in cultured and non-cultured amniotic fluid cells, in frozen sections of the placenta and in isolated cells of the amniotic epithelium. Two major classes of cells can be cultured from amniotic fluids, namely cells of epithelial origin containing filaments of the prekeratin type and cells of different origin which contain filaments of the vimentin type but are negative when tested with antibodies to epidermal prekeratin. The presence of prekeratin type filaments correlates with the morphology of colonies of amniotic fluid cell cultures in vitro as classified by Hoehn et al. (1974). Cells of E-type colonies are shown to be of epithelial origin. In contrast our data indicate a different origin of almost all cells of F-type colonies and of the large majority of cells of AF-type colonies. Cells of epithelial origin and positively stained with antibodies to epidermal prekeratin are occasionally scattered in F-type colonies and in variable percentages (up to 30%) in AF-type colonies. Surprisingly, cryostat sections of the amniotic epithelium and isolated groups of amniotic cells showed positive reactions with both antibodies to vimentin and prekeratin. The possibility that amniotic cells may be different from other epithelial cells in that they contain both types of filaments simultaneously already in situ is presently under investigation

Characterization of cells of amniotic fluids by immunological identification of intermediate-sized filaments: Presence of cells of different tissue origin

Antibodies against intermediate-sized filaments, of the prekeratin or vimentin type, were used to investigate the presence of these filaments by indirect immunofluorescence microscopy in cultured and non-cultured amniotic fluid cells, in frozen sections of the placenta and in isolated cells of the amniotic epithelium. Two major classes of cells can be cultured from amniotic fluids, namely cells of epithelial origin containing filaments of the prekeratin type and cells of different origin which contain filaments of the vimentin type but are negative when tested with antibodies to epidermal prekeratin. The presence of prekeratin type filaments correlates with the morphology of colonies of amniotic fluid cell cultures in vitro as classified by Hoehn et al. (1974). Cells of E-type colonies are shown to be of epithelial origin. In contrast our data indicate a different origin of almost all cells of F-type colonies and of the large majority of cells of AF-type colonies. Cells of epithelial origin and positively stained with antibodies to epidermal prekeratin are occasionally scattered in F-type colonies and in variable percentages (up to 30%) in AF-type colonies. Surprisingly, cryostat sections of the amniotic epithelium and isolated groups of amniotic cells showed positive reactions with both antibodies to vimentin and prekeratin. The possibility that amniotic cells may be different from other epithelial cells in that they contain both types of filaments simultaneously already in situ is presently under investigation

No dose-response effect of carbohydrate mouth rinse concentration on 5 km running performance in recreational athletes

Oral carbohydrate rinsing has been demonstrated to provide beneficial effects on exercise performance of durations of up to one hour, albeit predominately in a laboratory setting. The aim of the present study was to investigate the effects of different concentrations of carbohydrate solution mouth-rinse on 5 km running performance. Fifteen healthy men (n=9; mean±SD age: 42±10 years; height: 177.6±6.1 cm; body mass: 73.9±8.9 kg) and women (n=6; mean±SD age: 43±9 years; height: 166.5±4.1 cm; body mass: 65.7±6.8 kg) performed a 5 km running time trial on a track on four separate occasions. Immediately before starting the time trial and then after each 1 km, subjects rinsed 25 mL of either 0, 3, 6, or 12% maltodextrin for 10 s. Mouth-rinsing with 0, 3, 6 or 12% maltodextrin did not have a significant effect on the time to complete the time trial (0%: 26:34±4:07 min:sec; 3%: 27:17±4:33 min:sec; 6%: 27:05±3:52 min:sec; 12%: 26:47±4.31 min:sec; P=0.071;2 =0.15), heart rate (P=0.095; 2 =0.16), rating of perceived exertion (RPE) (P=0.195; P =0.11), blood glucose (P=0.920; P =0.01) and blood lactate concentration (P=0.831; 2 =0.02), with only non-significant trivial to small differences between concentrations. Results of this study suggest that carbohydrate mouth-rinsing provides no ergogenic advantage over that of an acaloric placebo (0%), and that there is no dose-response relationship between carbohydrate solution concentration and 5 km track running performance

Multiplex T-RFLP allows for increased target number and specificity : detection of Salmonella enterica and six species of Listeria in a single test

A multiplex T-RFLP test was developed to detect and identify Salmonella enterica and all six species of Listeria inoculated into milk at minimal levels. Extensive in silico analysis was used to design a fifteen-primer, six-amplimer methodology and in vitro application showed target organism DNA, when amplified individually, yielded the predicted terminal restriction fragments (TRFs) following digestion. Non-target organisms were either not-amplified or yielded TRFs which did not interfere with target identification. Multiple target DNA analysis gave over 86% detection of total TRFs predicted, and this was improved to over 90% detection of total TRFs predicted when only two target DNA extracts were combined analysed. Co-inoculation of milk with five strains each of the target species of S. enterica and L. monocytogenes, along with five strains of the non-target species E. coli was followed by enrichment in SEL medium for M-TRFLP analysis. This allowed for detection of both target species in all samples, with detection of one S. enterica and two Listeria TRFs in all cases, and detection of a second S. enterica TRF in 91% of cases. This was from an initial inoculum of <5 cfu per 25 ml milk with a background of competing E. coli present, and gave a result from sampling of under 20 hours. The ability to increase target species number without loss of sensitivity means that extensive screening can be performed at reduced cost due to a reduction in the number of tests required

On the locus formed by the maximum heights of projectile motion with air resistance

We present an analysis on the geometrical place formed by the set of maxima of the trajectories of a projectile launched in a media with linear drag. Such a place, the locus of apexes, is written in term of the Lambert $W$ function in polar coordinates, confirming the special role played by this function in the problem. In order to characterize the locus, a study of its curvature is presented in two parameterizations, in terms of the launch angle and in the polar one. The angles of maximum curvature are compared with other important angles in the projectile problem. As an addendum, we find that the synchronous curve in this problem is a circle as in the drag-free case.Comment: 7 pages, 6 color eps figures. Synchronous curve added. Typos and style corrected

Differences between US and German adults in native-born and migrants’ participation in volunteering as a dimension of civic engagement

We examine civic engagement among immigrants and native-born adults in the United States (U.S.) and Germany, operationalized in this study as voluntary work or volunteerism. Data for the analyses are drawn from the PIAAC. Germany and the U.S. were selected from the countries participating in PIAAC initial data collection in 2012 as two large OECD countries with different histories of immigration and responses to migrants. In Germany, native-born individuals showed greater volunteerism than immigrants (β = 0.119, p \u3c .001) while in the U.S., no significant difference was apparent by immigration status (β = 0.031, p = .133). These results are discussed in the paper relative to existing research literature and implications for adult education are suggested

Practitioner accounts and knowledge production: an analysis of three marketing discourses

Responding to repeated calls for marketing academicians to connect with marketing actors, we offer an empirically-sourced discourse analysis of the ways in which managers portray their practices. Focusing on the micro-discourses and narratives that marketing actors draw upon to represent their work we argue that dominant representations of marketing knowledge production present a number of critical concerns for marketing theory and marketing education. We also evidence that the often promoted idea of a need to close the gap between theory - as a dominant discourse - and practice, as a way of doing marketing, is problematic to pursue. We suggest that a more fruitful agenda resides in the development of a range of polyphonic and creative micro-discourses of management, promoting context, difference and individual meaning in marketing knowledge production