Machine Learning im CAE

Many companies have a large collection of different model variants and results. Hexagon's (formerly MSC Software) software Odyssee helps to find out what information is contained in this data. New calculations can sometimes be avoided because the results for new parameter combinations can be predicted from the existing calculations. This is particularly interesting for non-linear or large models with long run times. The software also helps when setting up new DOEs and offers a variety of options for statistical displays. In the lecture, the number-based and image-based methods are compared.Viele Firmen können auf eine große Sammlung vorhandener Rechnungen für verschiedene Modellvarianten zurückgreifen. Die Software Odyssee von Hexagon (früher MSC Software) hilft herauszufinden, welche Informationen in diesen Daten stecken. Neue Rechnungen kann man sich teilweise ersparen, weil die Ergebnisse für neue Parameterkombinationen aus den vorhandenen Rechnungen vorhergesagt werden können. Dies ist besonders interessant für nichtlineare oder große Modelle mit langer Rechenzeit. Die Software hilft auch beim Aufsetzen neuer DOEs und bietet vielfältige Möglichkeiten für statistische Darstellungen. In dem Vortrag werden die zahlenbasierte und bildbasierte Methode gegenübergestellt

Neurotophin Receptor p75NTR Regulates Immune Function of Plasmacytoid Dendritic Cells

Plasmacytoid dendritic cells (pDCs) regulate innate and adaptive immunity. Neurotrophins and their receptors control the function of neuronal tissue. In addition, they have been demonstrated to be part of the immune response but little is known about the effector immune cells involved. We report, for the first time, the expression and immune-regulatory function of the low affinity neurotrophin receptor p75 neurotrophin receptor (p75NTR) by the antigen-presenting pDCs, mediated by toll-like receptor (TLR) 9 activation and differential phosphorylation of interferon regulatory factor 3 and 7. The modulation of p75NTR on pDCs significantly influences disease progression of asthma in an ovalbumin-induced mouse model mediated by the TLR9 signaling pathway. p75NTR activation of pDCs from patients with asthma increased allergen-specific T cell proliferation and cytokine secretion in nerve growth factor concentration-dependent manner. Further, p75NTR activation of pDCs delayed the onset of autoimmune diabetes in RIP-CD80GP mice and aggravated graft-versus-host disease in a xenotransplantation model. Thus, p75NTR signaling on pDCs constitutes a new and critical mechanism connecting neurotrophin signaling and immune response regulation with great therapeutic potential for a variety of immune disorders

Machine Learning im CAE

Many companies have a large collection of different model variants and results. Hexagon's (formerly MSC Software) software Odyssee helps to find out what information is contained in this data. New calculations can sometimes be avoided because the results for new parameter combinations can be predicted from the existing calculations. This is particularly interesting for non-linear or large models with long run times. The software also helps when setting up new DOEs and offers a variety of options for statistical displays. In the lecture, the number-based and image-based methods are compared.Viele Firmen können auf eine große Sammlung vorhandener Rechnungen für verschiedene Modellvarianten zurückgreifen. Die Software Odyssee von Hexagon (früher MSC Software) hilft herauszufinden, welche Informationen in diesen Daten stecken. Neue Rechnungen kann man sich teilweise ersparen, weil die Ergebnisse für neue Parameterkombinationen aus den vorhandenen Rechnungen vorhergesagt werden können. Dies ist besonders interessant für nichtlineare oder große Modelle mit langer Rechenzeit. Die Software hilft auch beim Aufsetzen neuer DOEs und bietet vielfältige Möglichkeiten für statistische Darstellungen. In dem Vortrag werden die zahlenbasierte und bildbasierte Methode gegenübergestellt

Machine Learning im CAE

Many companies have a large collection of different model variants and results. Hexagon's (formerly MSC Software) software Odyssee helps to find out what information is contained in this data. New calculations can sometimes be avoided because the results for new parameter combinations can be predicted from the existing calculations. This is particularly interesting for non-linear or large models with long run times. The software also helps when setting up new DOEs and offers a variety of options for statistical displays. In the lecture, the number-based and image-based methods are compared.Viele Firmen können auf eine große Sammlung vorhandener Rechnungen für verschiedene Modellvarianten zurückgreifen. Die Software Odyssee von Hexagon (früher MSC Software) hilft herauszufinden, welche Informationen in diesen Daten stecken. Neue Rechnungen kann man sich teilweise ersparen, weil die Ergebnisse für neue Parameterkombinationen aus den vorhandenen Rechnungen vorhergesagt werden können. Dies ist besonders interessant für nichtlineare oder große Modelle mit langer Rechenzeit. Die Software hilft auch beim Aufsetzen neuer DOEs und bietet vielfältige Möglichkeiten für statistische Darstellungen. In dem Vortrag werden die zahlenbasierte und bildbasierte Methode gegenübergestellt

Generation of inducible immortalized dendritic cells with proper immune function in vitro and in vivo.

Dendritic cells are the professional antigen presenting cells of innate immunity and key players in maintaining the balance of immune responses. Studies with dendritic cells are mainly limited by their low numbers in vivo and their difficult maintenance in vitro. We differentiated bone marrow cells from transgenic mice expressing an inducible SV40 large T-antigen into dendritic cells. When immortalized by dexamethasone and doxycycline, these cells were stable in long-term culture. In the absence of dexamethasone and doxycycline (de-induction), dendritic cells displayed properties of primary cells, characterized by expression of classical dendritic cell surface markers CD11c, CD11b, MHCII, CD40 and CD86. Furthermore, de-induced lipopolysaccharide activated dendritic cells secreted IL-1β, IL-6, TNFα and IL-12. De-induced, Ovalbumin-loaded dendritic cells polarize CD4(+) T cells into Th1, Th17 and Th2 cells, indicating their correct antigen presenting property. Consistent with intratracheal application of Ovalbumin-loaded primary dendritic cells into mice, the application of de-induced dendritic cells resulted in recruitment of lymphocytes to the lungs. In summary, we successfully expanded dendritic cells using conditional immortalization. The generated dendritic cells demonstrate the characteristic immunophenotype of primary dendritic cells and will facilitate further studies on immunomodulatory properties of dendritic cells