Ethnopharmacological study on plants used for skincare and beauty by some Xhosa communities.

Doctoral Degree. University of KwaZulu-Natal, Pietermaritzburg.The paraphrase “beauty lies in the eyes of the beholder” by Plato, a Greek philosopher, has echoed through the fabrics of time and still echoes in our generation. The statement simply ought to refer to that the observer gets to decide what is “beautiful” in their eyes. The decision on what and who is beautiful is heavily influenced by one’s surroundings. In a digital-market environment the concept of beauty enhancement is paraded by bigger forces to drive their own agenda. The desire to enhance one’s facial appearance has significantly contributed to the observed growth of the beauty industry. The notable growth of the industry has in some cases resulted in unpleasant consequences due to the side effects of some of the products in the market. In this global-blooming industry products are traded and exchanged at a rapid rate. Introduction of safer natural beauty enhancement products are required for the South African market if we were to alleviate those with undesirable side effects and could combat some socio-economic challenges through job creation. The use of plants as the source of natural compounds has proven to be a reliable strategy in ethnopharmacological applications. The Eastern Cape Province has a rich plant biodiversity and the communities have immense indigenous knowledge (IK) on the use of these plants. There is a need to explore the pharmacological application of these plants by introducing beauty enhancement product formulations made from local resources. The project was aimed at documenting and conductingethnopharmacological evaluation of plants used for skincare and beauty for their potential in formulation of beauty enhancement products. An ethnobotanical survey was conducted to document plants that are used by communities in the Raymond Mhlaba municipality for skincare and beauty. Knowledge holders were identified by purpose sampling method and the interviews were conducted in isiXhosa using a structured questionnaire. Information on demographics, names of the plant, type of plant, plant part used, method of preparation and administration and frequency of use was collected and captured in the questionnaires. The Asphodelaceae and Asteraceae were the most represented families of the plants used for skincare and beauty. The communities used sustainable harvesting practices as the leaves were the most utilized plant parts. The most reported beauty enhancement uses were for achieving desired skin complexion and for skin smoothness, with both accounting up to 50% of the reported plant usages. Sixteen plants with the highest frequency index (FI) were selected from the ethnobotanical survey for ethnopharmacological studies related to beauty enhancement. This included Acokanthera oblongifolia (Hochst.) Codd, Aloe ferox Mill, Arctotis arctotoides (L.f) O.Hoffm, Bulbine frutescens (L.) Willd, Cassipourea flanaganii (Schinz) Alston, Chenopodium album L, Clausena anisata (Willd.) Hook.f ex Benth, Haemanthus albiflos Jacq, Marrubium vulgare L, Ilex mitis (L.) Radlk, Plantago lanceolata L, Rorippa nasturtium-aquaticum (L.) Hayek, Sonchus asper L, Symphytum officinale L, Ruta graveolens L and Urtica urens L. The antimicrobial activity of plant extracts was assessed using the microdilution bioassay to determine the minimum inhibitory concentrations (MIC). The antimicrobial activity of petroleum ether (PE), dichloromethane (DCM), 70% aqueous ethanol (v/v) and water extracts of the selected plants were assessed against infectious skin microorganisms including Bacillus subtilis ATCC 6051, Brevibacillus agric ATCC 51663, Staphylococcus aureus ATCC 12600, Staphylococcus epidermidis ATCC 12228, Escherichia coli ATCC 11775, Klebsiella pneumoniae ATCC 13883, Candida albicans ATCC 10231 and the dermatophytes Microsporum canis ATCC 36299, Trichophyton mentagrophytes ATCC 9533 and Trichophyton tonsurans ATCC 28942. The majority of the tested plant extracts were effective and inhibited the skin commensal bacteria E. coli with MIC values less than 100 μg/mL. Prolonged infections by commensal bacteria can condition the skin environment and provide favourable conditions for more opportunistic bacteria such as the Staphylococci genus. Ethanol extracts of C. flanaganii and U. urens expressed high antibacterial activity against S. aureus with MIC values less than 100 μg/mL. Ethanol extracts of R. graveolens and dichloromethane extracts of A. arctotoides were effective at inhibiting S. epidermidis and S. aureus, respectively. Inhibition of two opportunistic bacteria has a positive effect on skin tone, due to the scarring and darkening associated with infection by the Staphylococci genus. There was notable activity recorded against C. albicans and dermatophytes M. canis, T. mentagrophytes and T. tonsurans by extracts of A. oblongifolia, A. arctotoides, C. flanaganii, I. mitis and R. graveolens at different polarities with MIC’s less than 1000 μg/mL. The phenolic content and antioxidant activity of the plants were determined by assessing 50% aqueous methanol extracts (v/v) for their total phenolic and flavonoid content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power (FRAP) and the coupled oxidation of linoleic acid and bleaching of β-carotene. The antioxidant mechanism of phenolic compounds associated with beauty enhancement has been proposed to be due to their free radical chain breaking capabilities, metal chelation, oxidant quenching and inhibition of enzymatic activity. The total phenolic content of A. ferox, I. mitis and C. flanaganii were significantly high with recorded values ranging from 37.87 to 50.34 mgGAE/g. The flavonoid content of C. flanaganii, A. oblongifolia and P. lanceolata were significantly high. Methanol extracts of R. graveolens and C. flanaganii expressed the highest antioxidant activity, with IC50 values comparable to the standard antioxidant when assessed for their DPPH radical scavenging activity. The presence of antioxidants in the skin structural layers has a positive effect on the health and function of the skin. Extracts of U. urens, A. ferox, C. flanaganii, B. frutescens, P. lanceolata, H. albiflos, M. vulgare, C. anisata, S. officinale and R. nasturtium-aquaticum expressed good metal chelating potential. The highest oxidative protection in the β-carotene linoleic acid model with comparative oxidation rate ratio (ORR) to the positive control was observed for C. flanaganii, S. officinale and U. urens. The results indicate the ability of the plant extracts to provide protection against increased levels of lipid peroxidation in the skin, an important factor in beauty enhancement, due to delaying the age process. The photo-protective effect of the plant extracts was measured by calculating the sun protection factor (SPF). The SPF is the ratio of ultraviolet (UV) radiation required to produce minimal erythema dose (MED) in protected skin to unprotected skin with higher values indicative of increased protection from photo damage. Ethanol extracts of P. lanceolata, C. flanaganii, A. oblongifolia, I. mitis and A. arctotoides exhibited SPF values of more than 15, which translated to photo protection of the skin against UVB radiation by more than 93.3%. The plant extracts demonstrated the highest absorbance of UVB radiation at a wavelength region between 300 – 305 nm. These will protect the skin against UV-induced oxidative damage and enhance the skin’s health and function. The inhibition of enzymes with beauty enhancement potential by the plant extracts was assessed against tyrosinase, secretory phospholipase A2 (sPLA2), lipoxygenase (15-LOX) and cyclooxygenase (COX-1 and COX-2). Ethanol extracts of R. nasturtium-aquaticum, C. anisata, S. officinale and C. flanaganii expressed good anti-tyrosinase activity. The coupled protection against UV-induced damage and modulation of the tyrosinase enzyme activity can be exploited to achieve the desired skin complexion. The anti-inflammatory studies revealed the potential of extracts of C. flanaganii, P. lanceolata and R. nasturtium-aquaticum to serve as dual inhibitors of 15-LOX and COX-2 enzymes. The inhibition of 15-LOX and COX-2 is effective at resolving psoriasis, a skin-inflammatory associated disease which has a negative effect on the health and beauty of the skin. The effectiveness of ethanol extracts of C. flanaganii, C. album, C. anisata and R. nasturtium-aquaticum in maintaining the cells health and function was examined on human epidermal melanocytes (HEM) cell lines. Ethanol extracts of C. flanaganii, C. album, C. anisata and R. nasturtium-aquaticum were able to inhibit cellular tyrosinase activity and therefore reduce melanin production. The effective concentrations of the extracts were further reported as non-toxic to melanocytes. The observed anti-tyrosinase activity of the extracts against HEM cell lines contribute positively in achieving the desired skin complexion while providing photo protection against UV-induced damage. Therefore, plant extracts that are efficient and safe to use can be incorporated into formulations intended for beauty enhancement and further analysed under clinical trials. The study further suggests that the model undertaken be promoted to individuals and corporations interested in formulation of cosmeceuticals to ensure the safety and efficiency of their products

Response of the IMF and the World Bank to the Great Recession and the Euro sovereign crisis in a globalising world

The International Monetary Fund (IMF) and the International Bank for Reconstruction and Development (IBRD) now the World Bank, were created in the mid-1940s. The IMF was tasked to manage the post-war international monetary system, while the World Bank’s role during its early years was to provide development finance to war-torn Europe. These institutions reformed some of their roles to make them relevant to the globalising world over the years and also responded to several post-war crises. Since these two institutions carry out their roles in a globalising world, this study has revealed that globalisation has different interpretations as many researchers refer to the economic and non-economic explanations of its meaning. Globalisation is also a historical process as it traces back several years ago. Since approximately the mid-2000s, the global economy experienced two economic crises, namely the US sub-prime financial crisis that later became the Great Recession and the Euro sovereign crisis. The two economic crises spread to other countries globally that were interconnected into the global economy regarding international trade, investment and banking. These two crisis events required responses from the IMF and the World Bank. The two institutions displayed a variety of strengths and weaknesses in dealing with the recession and the Euro crisis. The lending of both these institutions has been their strength as they have managed to expand their lending capacity during the two crisis periods examined. The IMF’s crisis intervention time frames have also been its strength, as the speed in which it has approved financial assistance requests has been within reasonable time frames. The IMF’s new lending instruments have been its weakness, as the success of these instruments has not been fully tested so far. This is because of the little use of the IMF’s new lending instruments. The IMF’s crisis prevention efforts through the use of its surveillance tools have also been its flaw. This is based on the fact that it has failed to prevent the US financial crisis (later the Great Recession) and the Euro sovereign crisis. Overall, this study found that these institutions played a significant role in responding to the Great Recession and Euro sovereign crisis as their strengths outweigh their weaknesses. However, the weaknesses of the IMF confirm that it needs to reform its role and learn from its flaws in the future

Economic and market analysis of smallholder farmers' broiler production in the Amatole district municipality, Eastern Cape, South Africa.

Masters Degree. University of KwaZulu-Natal, Pietermaritzburg.South Africa is a major broiler producer in Sub-Saharan Africa (SSA) accounting for 80% of total broiler production in the region. Broiler production also dominates the agricultural sector in South Africa and remains the cheapest source of protein relative to other animal proteins. The broiler industry has a dual nature, which consists of both smallholder and commercial broiler farmers. Feed is a major cost issue in the broiler sector, accounting for more than 70%of a broiler producer's overall costs. Another obstacle faced by smallholder broiler farmers, especially those in the Amatole District Municipality (ADM) in the Eastern Cape, is the issue of market access. The aim of the study was to carry out the economic and market analysis of smallholder farmers broiler production in the Amatole District Municipality, Eastern Cape, South Africa .The main objectives of the study, which were used to address the research problem were; to identify and profile broiler farming, determine determinants of market participation among smallholder broiler producers, analyse the factors affecting smallholder broiler farmers’ profitability, and analyse the factors affecting smallholder broiler farmers’ profitability in the Amatole District Municipality in the Eastern Cape, South Africa. A sample of 150 smallholder broiler farmers was drawn and primary data was collected using a structured questionnaire. Descriptive statistics were used to profile the broiler farmers in ADM. The average smallholder broiler farmer in ADM was middle-aged and a female. The double-hurdle model was used to analyse factors affecting market participation decisions. The first stage made use of the probit model to analyse factors affecting the probability of market participation. The second stage involves the assessment of factors affecting market participation intensity by the smallholder broiler farmers in ADM. The marketing constraints were also analysed. Determining the factors affecting the profitability also involved two stages. The first stage made use of the budgetary technique (Gross margin and net farm income) to measure smallholder broiler profits. The results indicated that smallholder broiler production in ADM was profitable, this was indicated by the net farm income of ZAR 996.16 per production cycle. The second stage analysed factors influencing farm profitability. The stochastic production frontier together with the technical efficiency was used to measure the broiler farmers’ productivity. The average technical efficiency of farmers was found to be 0.81 indicating that the average smallholder broiler farmer in ADM is technically efficient. Broiler farmers in the ADM were encouraged to increase their stock size to allow for an increase in their probability to enter markets. Keywords: broiler market, market participation, productivity, profitabilit

Effect of Marine Polyunsaturated Fatty Acids on Biofilm Formation of Candida albicans and Candida dubliniensis

The effect of marine polyunsaturated fatty acids on biofilm formation by the human pathogens Candida albicans and Candida dubliniensis was investigated. It was found that stearidonic acid (18:4 n-3), eicosapentaenoic acid (20:5 n-3), docosapentaenoic acid (22:5 n-3) and docosahexaenoic acid (22:6 n-3) have an inhibitory effect on mitochondrial metabolism of both C. albicans and C. dubliniensis and that the production of biofilm biomass by C. dubliniensis was more susceptible to these fatty acids than C. albicans. Ultrastructural differences, which may be due to increased oxidative stress, were observed between treated and untreated cells of C. albicans and C. dubliniensis with formation of rough cell walls by both species and fibrillar structures in C. dubliniensis. These results indicate that marine polyunsaturated fatty acids may be useful in the treatment and/or prevention of biofilms formed by these pathogenic yeasts

Metabolite Profile of Athrixia phylicoides DC. (Bush Tea) and Determination of Inhibitory Mechanism against Tyrosinase Enzyme from Mushroom

Athrixia phylicoides DC. (Bush tea) is a shrub harvested in the north-eastern mountain regions of South Africa and belongs to the Asteraceae family. Generally, A. phylicoides is consumed as a hot tea beverage for its associated health benefits. The use of bush tea extracts for beauty enhancement has not been investigated even though several ethnobotanical reports have indicated its usage against skin imperfections. Therefore, the aim of the study was to assess plant metabolites of A. phylicoides for their inhibition of tyrosinase from mushroom and determine their inhibitory mechanism. Methanolic extracts (80% v/v) of A. phylicoides were evaluated using a tyrosinase-based TLC (thin-layer chromatography) autography technique. The inhibitory mechanism of active metabolites against the enzyme was determined using Lineweaver–Burk plots. Quercetin and an unknown metabolite with a retention factor (Rf) value of 0.73 inhibited melanogenesis. However, the IC50 value for Quercetin was reported as 51.07 ± 2.43 µg/mL higher than that of kojic acid of 5.22 ± 1.44 µg/mL. Chlorogenic acid was reported to have a similar Rf value as kojic acid, suggesting similarities in polarity and affinity towards the adsorbent material. However, chlorogenic acid with an IC50 value of 15.25 ± 1.18 µg/mL and an inhibitory strength of 2.92 could not inhibit melanogenesis with a similar intensity as kojic acid. The inhibitory mechanism for A. phylicoides extract was reported to be mixed inhibition (competitive/uncompetitive). The IC50 value for A. phylicoides was recorded as 20.65 ± 0.14 µg/mL with an inhibitory strength of 3.96. These results suggest that A. phylicoides extracts could be used against dark spots associated with scarring and ageing through modulation of tyrosinase activity

Metabolite Profile of <i>Athrixia phylicoides</i> DC. (Bush Tea) and Determination of Inhibitory Mechanism against Tyrosinase Enzyme from Mushroom

Athrixia phylicoides DC. (Bush tea) is a shrub harvested in the north-eastern mountain regions of South Africa and belongs to the Asteraceae family. Generally, A. phylicoides is consumed as a hot tea beverage for its associated health benefits. The use of bush tea extracts for beauty enhancement has not been investigated even though several ethnobotanical reports have indicated its usage against skin imperfections. Therefore, the aim of the study was to assess plant metabolites of A. phylicoides for their inhibition of tyrosinase from mushroom and determine their inhibitory mechanism. Methanolic extracts (80% v/v) of A. phylicoides were evaluated using a tyrosinase-based TLC (thin-layer chromatography) autography technique. The inhibitory mechanism of active metabolites against the enzyme was determined using Lineweaver–Burk plots. Quercetin and an unknown metabolite with a retention factor (Rf) value of 0.73 inhibited melanogenesis. However, the IC50 value for Quercetin was reported as 51.07 ± 2.43 µg/mL higher than that of kojic acid of 5.22 ± 1.44 µg/mL. Chlorogenic acid was reported to have a similar Rf value as kojic acid, suggesting similarities in polarity and affinity towards the adsorbent material. However, chlorogenic acid with an IC50 value of 15.25 ± 1.18 µg/mL and an inhibitory strength of 2.92 could not inhibit melanogenesis with a similar intensity as kojic acid. The inhibitory mechanism for A. phylicoides extract was reported to be mixed inhibition (competitive/uncompetitive). The IC50 value for A. phylicoides was recorded as 20.65 ± 0.14 µg/mL with an inhibitory strength of 3.96. These results suggest that A. phylicoides extracts could be used against dark spots associated with scarring and ageing through modulation of tyrosinase activity

Ethnobotany and Toxicity Status of Medicinal Plants with Cosmeceutical Relevance from Eastern Cape, South Africa

The indigenous people of the Eastern Cape residing within the richest plant biodiversity in the world, including Africa&rsquo;s floral &lsquo;gold mine&rsquo;, have a long history of plant use for skincare. However, such rich flora comes with numerous plants that have the potential to cause harm to humans through their usage. Therefore, the study was aimed at documenting the toxicity status of important medicinal plants used by the indigenous people from the Eastern Cape for skincare and supported by literature for cosmeceutical relevance. A list of plants used for skincare was produced following an ethnobotanical survey. In addition, data on the level of toxicity and cosmeceutical relevance of plants listed from the survey were collected from literature resources. The study listed a total of 38 plants from 25 plant families, the majority being represented by the Asphodelaceae and Asteraceae, both at 13.2%. The most preferred plant parts were the leaves (60.4%) indicating sustainable harvesting practices by the community. The literature reports validated 70% of the medicinal plants surveyed for skincare were nontoxic. Most of the plants can be incorporated in the formulation of products intended for skincare due to their low toxicity and high cosmeceutical relevance