Б1.В.ОД.15.4 Этология и зоопсихология 2018 очная

Increased apoptotic activity in xenograft tumors (day 35) treated with miR-410 relative to miR-NC, assessed by caspase 3/7 ELISA assay (Promega). (PPTX 50.3 kb

Blood and biochemical results.

<p>Blood and biochemical results.</p

Expression of Toll-like receptors (TLRs) in the lungs of an experimental sepsis mouse model - Fig 2

<p><b>a-d)</b> Analysis of lung tissues through IF showed a significantly increased area (%) of expression of TLRs 2, 3, 4 and 7 in all S-groups compared to the time-adjusted C-groups (p<0.05), <b>e)</b> Among septic mice, expression of TLRs 2, 3 and 4 was higher in S48 versus S24 groups (p<0.05), S72 versus S24 groups (p<0.05) and S72 versus S48 groups whereas expression of TLR 7 was higher in S48 versus S24 groups (p<0.05), S72 versus S24 groups (p<0.05), but yet comparable between S72 and S48 groups (p>0.05).</p

Analysis of lung tissues through qRT-PCR showed S-groups yielded a significantly higher expression of all TLRs in S-groups compared to their time-adjusted C-groups (p<0.05).

<p>The most significant variances were observed between S72-C72 followed by S48-C48 groups concerning TLR 2 and TLR 4, whereas the least important differences were noticed between S24-C24 regarding TLR 7 and TLR 3.</p

Among S-groups, qRT-PCR of intestinal tissues showed significant differences between S48-S24 regarding TLR 2, 4 and 7 (p<0.05) as well as between S72-S48 and S72-S24 for all TLRs examined (p<0.05).

<p>Among S-groups, qRT-PCR of intestinal tissues showed significant differences between S48-S24 regarding TLR 2, 4 and 7 (p<0.05) as well as between S72-S48 and S72-S24 for all TLRs examined (p<0.05).</p

Age and frailty are independently associated with increased COVID-19 mortality and increased care needs in survivors: results of an international multi-centre study

Introduction: Increased mortality has been demonstrated in older adults with coronavirus disease 2019 (COVID-19), but the effect of frailty has been unclear. Methods: This multi-centre cohort study involved patients aged 18 years and older hospitalised with COVID-19, using routinely collected data. We used Cox regression analysis to assess the impact of age, frailty and delirium on the risk of inpatient mortality, adjusting for sex, illness severity, inflammation and co-morbidities. We used ordinal logistic regression analysis to assess the impact of age, Clinical Frailty Scale (CFS) and delirium on risk of increased care requirements on discharge, adjusting for the same variables. Results: Data from 5,711 patients from 55 hospitals in 12 countries were included (median age 74, interquartile range [IQR] 54–83; 55.2% male). The risk of death increased independently with increasing age (>80 versus 18–49: hazard ratio [HR] 3.57, confidence interval [CI] 2.54–5.02), frailty (CFS 8 versus 1–3: HR 3.03, CI 2.29–4.00) inflammation, renal disease, cardiovascular disease and cancer, but not delirium. Age, frailty (CFS 7 versus 1–3: odds ratio 7.00, CI 5.27–9.32), delirium, dementia and mental health diagnoses were all associated with increased risk of higher care needs on discharge. The likelihood of adverse outcomes increased across all grades of CFS from 4 to 9. Conclusion: Age and frailty are independently associated with adverse outcomes in COVID-19. Risk of increased care needs was also increased in survivors of COVID-19 with frailty or older age.</p