Simulating the impact of water demand management options on water consumption and wastewater generation profiles

This is the final version. Available on open access from Taylor & Francis via the DOI in this recordThis paper describes the development and application of a simulation tool to assess the impact of water-efficient appliances and greywater recycling strategies on the domestic water consumption and wastewater generation profiles. Two time-series datasets of domestic water consumption in UK households – each consisting of more than 20,000 observations – were used to deduce water use appliance usage patterns as a function of water consumption per use for each water-use appliance and household characteristic (e.g. occupancy). The deduced trends were then used to develop a simulation tool that can generate water consumption and wastewater generation profiles reflecting user input for water use appliances and household characteristics. The inbuilt flexibility in the tool allows investigation of each water use appliance both individually and collectively, and facilitates informed decision-making for devising targeted retrofitting programmes. This research has application for water service provision and policy making globally, with potential impact on both water supply and wastewater management

The Pan-Genome of the Animal Pathogen Corynebacterium pseudotuberculosis Reveals Differences in Genome Plasticity between the Biovar ovis and equi Strains

Soares SC, Silva A, Trost E, et al. The Pan-Genome of the Animal Pathogen Corynebacterium pseudotuberculosis Reveals Differences in Genome Plasticity between the Biovar ovis and equi Strains. PLoS ONE. 2013;8(1): e53818.Corynebacterium pseudotuberculosis is a facultative intracellular pathogen and the causative agent of several infectious and contagious chronic diseases, including caseous lymphadenitis, ulcerative lymphangitis, mastitis, and edematous skin disease, in a broad spectrum of hosts. In addition, Corynebacterium pseudotuberculosis infections pose a rising worldwide economic problem in ruminants. The complete genome sequences of 15 C. pseudotuberculosis strains isolated from different hosts and countries were comparatively analyzed using a pan-genomic strategy. Phylogenomic, pan-genomic, core genomic, and singleton analyses revealed close relationships among pathogenic corynebacteria, the clonal-like behavior of C. pseudotuberculosis and slow increases in the sizes of pan-genomes. According to extrapolations based on the pan-genomes, core genomes and singletons, the C. pseudotuberculosis biovar ovis shows a more clonal-like behavior than the C. pseudotuberculosis biovar equi. Most of the variable genes of the biovar ovis strains were acquired in a block through horizontal gene transfer and are highly conserved, whereas the biovar equi strains contain great variability, both intra- and inter-biovar, in the 16 detected pathogenicity islands (PAIs). With respect to the gene content of the PAIs, the most interesting finding is the high similarity of the pilus genes in the biovar ovis strains compared with the great variability of these genes in the biovar equi strains. Concluding, the polymerization of complete pilus structures in biovar ovis could be responsible for a remarkable ability of these strains to spread throughout host tissues and penetrate cells to live intracellularly, in contrast with the biovar equi, which rarely attacks visceral organs. Intracellularly, the biovar ovis strains are expected to have less contact with other organisms than the biovar equi strains, thereby explaining the significant clonal-like behavior of the biovar ovis strains

Chemical Composition of Polymer Surfaces Imaged by Atomic Force Microscopyand Complementary Approaches

Unfortunately, the editor of this article Hans-Henning Kausch was published as author in the XML-version