379 research outputs found

    Speciation dynamics in the SE Asian tropics: Putting a time perspective on the phylogeny and biogeography of Sundaland tree squirrels, Sundasciurus

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    Tropical rainforests are well known for their extraordinarily high levels of biodiversity. The origin of this species richness is still debated. For instance, the museum hypothesis states that over evolutionary time more and more species will accumulate with relatively few extinctions. In contrast, the Pleistocene diver- siïŹcation model argues that during the last 2 million years, climatic factors (glaciations) caused environ- mental changes that drove isolation and vicariant speciation events. In this study, we construct a molecular phylogeny of the Sundaland (Malay Peninsula, Sumata, Borneo, Palawan) and Greater Minda- nao (Mindanao, Samar, Leyte) tree squirrels (genus Sundasciurus). Our results show that most speciation events in this forest dependent taxon occurred before the Pleistocene and that even the timing of intra- speciïŹc splits among populations from different landmasses are relatively old. Additionally, we found unexpectedly high divergence within and between highland populations of S. tenuis on Sumatra and the Malay Peninsula, highlighting the importance of Pliocene events in both speciation and within species divergences in this regionPeer reviewe

    The Texas Nature Tracker

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    A newsletter providing information about environmental monitoring, endangered species, and nature conservation within Texas

    Diagonal conditions in ordered spaces

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    Abstract. For a space X and a regular uncountable cardinal Îș ≀ |X| we say that then we say that X has a small diagonal, and if every regular uncountable Îș ≀ |X| belongs to D(X) then we say that X has an H-diagonal. In this paper we investigate the interplay between D(X) and topological properties of X in the category of generalized ordered spaces. We obtain cardinal invariant theorems and metrization theorems for such spaces, proving, for example, that a Lindelöf linearly ordered space with a small diagonal is metrizable. We give examples showing that our results are the sharpest possible, e.g., that there is a first countable, perfect, paracompacĆ„ Cech-complete linearly ordered space with an H-diagonal that is not metrizable. Our example shows that a recent CH-result of JuhĂĄsz and SzentmiklĂłssy on metrizability of compact Hausdorff spaces with small diagonals cannot be generalized beyond the class of locally compact spaces. We present examples showing the interplay of the above diagonal conditions with set theory in a natural extension of the Michael line construction

    Selective Enrichment and Sequencing of Whole Mitochondrial Genomes in the Presence of Nuclear Encoded Mitochondrial Pseudogenes (Numts)

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    Numts are an integral component of many eukaryote genomes offering a snapshot of the evolutionary process that led from the incorporation of an α-proteobacterium into a larger eukaryotic cell some 1.8 billion years ago. Although numt sequence can be harnessed as molecular marker, these sequences often remain unidentified and are mistaken for genuine mtDNA leading to erroneous interpretation of mtDNA data sets. It is therefore indispensable that during the process of amplifying and sequencing mitochondrial genes, preventive measures are taken to ensure the exclusion of numts to guarantee the recovery of genuine mtDNA. This applies to mtDNA analyses in general but especially to studies where mtDNAs are sequenced de novo as the launch pad for subsequent mtDNA-based research. By using a combination of dilution series and nested rolling circle amplification (RCA), we present a novel strategy to selectively amplify mtDNA and exclude the amplification of numt sequence. We have successfully applied this strategy to de novo sequence the mtDNA of the Black Field Cricket Teleogryllus commodus, a species known to contain numts. Aligning our assembled sequence to the reference genome of Teleogryllus emma (GenBank EU557269.1) led to the identification of a numt sequence in the reference sequence. This unexpected result further highlights the need of a reliable and accessible strategy to eliminate this source of error
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