Intervention data REDHOT cluster randomized trial (2012)

Individual-level data from cross-sectional surveys prior to hotspot-targeted interventions and post-intervention. Data can be linked to intervention coverage data using compound and household identifiers. Data was collected in 2012

REDHOT baseline survey 2011

Individual-level baseline data that were collected in preparation of a hotspot-targeted cluster randomized trial. Data include household and ecological characteristics, parasite prevalence by P. falciparum nPCR and antibody responses to P. falciparum antigens AMA-1 and MSP-1

Entomology data REDHOT

CDC light trap mosquito collections in 3 intervention and 3 control clusters. Number of female, male and total number of anopheles and culex mosquitoes is given per sampling round and compound. Up to 9 sampling rounds were completed per compound before (n=1) and after (n=8) the intervention

Membrane feeding assays

The results of the membrane feeding experiments performed during the different infectivity surveys are included in this dataset. In addition to data on the feeding experiments, information on the age of study participants is included

MOESM5 of Assessing Plasmodium falciparum transmission in mosquito-feeding assays using quantitative PCR

Additional file 5. Detection of low intensity midgut infections. EHV, RPS7, and 18S Cp values for 12 P. falciparum positive and two negative mosquito midgut controls, as well as numbers of oocysts identified by microscopy. Acceptable QC range was defined as within 2 SD of the mean Cp. *Inhibited extraction or PCR. Midgut 1-6, 8-13 were positively infected. Midgut 7 & 14 were uninfected midguts

MOESM4 of Assessing Plasmodium falciparum transmission in mosquito-feeding assays using quantitative PCR

Additional file 4. Cp values obtained using the RPS7 PCR assay on DNA extracts from whole A. stephensi mosquitoes and mosquito midguts. The preliminary evaluation of RPS7 PCR assay successfully amplified DNA extracted from 5 whole A. stephensi mosquitoes and 15 mosquito midguts, producing mean Cp of 19.2 for whole mosquito DNA extract Cp of 23.6 for midgut extracts

MOESM6 of Assessing Plasmodium falciparum transmission in mosquito-feeding assays using quantitative PCR

Additional file 6. 18S ddPCR results compared with qPCR in microscopy confirmed oocyst-positive midguts. Comparison of qPCR and ddPCR quantification of oocyst-positive midguts from a CHMI study. The median genome number per oocysts were estimated to be 3,722 genomes/oocyst by qPCR and 3,490 genomes/oocyst by ddPCR

MOESM3 of Assessing Plasmodium falciparum transmission in mosquito-feeding assays using quantitative PCR

Additional file 3. 18S qPCR validation data using syn18S DNA standard dilution series performed on 3 separate occasions. HGD – human genomic DNA (uninfected human blood extracts) as negative controls. ND = not detected. Average linear regression from 3 PCR runs Cp=-3.4541*log(conc)+36.1006

Characteristics of membrane feeding assay blood donors and mosquito infections

Model takes raw QT-NASBA data from Schneider et al. 2007 (doi:10.5061/dryad.589ft) and Ouédraogo et al. 2009 (doi:10.5061/dryad.hv01f) and fits a mathematical model to estimate the gametocyte density (gam.median) and 95% Bayesian Credible Intervals (gam.lower, gam.upper). These are combined with blood donor and mosquito characteristics from the membrane feeding assays. Column “Host” denotes host id, “Experiment” gives country (0=Burkina, 1=Kenya), age (0=<7 or 1=>=7 years old) and “asexual.cat” is the asexual parasite density category as measured by microscopy (0=none,1=low,2=high). The point estimate for prevalence of oocysts is given from the model outputs “prev.median” together with 95% Bayesian Credible Intervals (prev.lower, prev.upper). Full details are given in the manuscript

Risk factors for the mean total number of indoor vectors per household collected over three occasions.

1<p>Incidence rate ratios (IRRs) estimated by random effects negative binomial regression.</p><p>Dependent variable is the mean total number of <i>Anopheles gambiae</i> and <i>funestus</i> caught per household over three occasions.</p>***<p>p<0.01, **p<0.05, *p<0.1.</p