Additional file 4. Cp values obtained using the RPS7 PCR assay on DNA extracts from whole A. stephensi mosquitoes and mosquito midguts. The preliminary evaluation of RPS7 PCR assay successfully amplified DNA extracted from 5 whole A. stephensi mosquitoes and 15 mosquito midguts, producing mean Cp of 19.2 for whole mosquito DNA extract Cp of 23.6 for midgut extracts
