Additional file 5. Detection of low intensity midgut infections. EHV, RPS7, and 18S Cp values for 12 P. falciparum positive and two negative mosquito midgut controls, as well as numbers of oocysts identified by microscopy. Acceptable QC range was defined as within 2 SD of the mean Cp. *Inhibited extraction or PCR. Midgut 1-6, 8-13 were positively infected. Midgut 7 & 14 were uninfected midguts
