Muisti ja Arki : Ajankohtaista tietoa muistisairauksista, niiden ennaltaehkäisemisestä sekä arjesta muistisairauden kanssa

Suomen väestön ikärakenne muuttuu nopeasti lähivuosikymmeninä. Tilastokeskuksen ennusteiden mukaan yli 75-vuotiaiden määrä kaksinkertaistuisi vuoteen 2030 mennessä, joten muistisairauksien esiintyvyys tulee myös lisääntymään ikääntyvien määrän kasvaessa. Opinnäytetyömme idea sai alkunsa SenioriKaste-hankkeelta, joka toivoi ensitietopäivän järjestämistä Keski-Pohjanmaan alueella muistisairauksiin sairastuneille sekä heidän läheisilleen. Opinnäytetyömme tarkoituksena oli järjestää muistisairauksia käsittelevä tilaisuus muistisairauteen sairastuneille sekä heidän läheisilleen. Tilaisuus oli myös avoin kaikille asiasta kiinnostuneille. Opinnäytetyömme tavoitteena oli välittää muistisairauteen sairastuneille ja heidän läheisilleen ajankohtaista tietoa muistisairauksista sekä keinoista, joiden avulla kotona pärjääminen onnistuu mahdollisimman pitkään. Kartoitimme yhteistyökumppaneidemme kautta aiheen tärkeyttä ja tarvetta. Heidän asiakkailtaan saadun palautteen perusteella varmistuimme tilaisuuden järjestämisen tärkeydestä ja tiedon tarpeellisuudesta. Opinnäytetyömme toteutui toiminnallisena projektityönä yhteistyössä toimeksiantajan SenioriKaste-hankkeen kanssa Kokkolassa 4.6.2015. Tilaisuudessa luennoitiin muistisairauksien ennaltaehkäisemisestä, yleisimmistä muistisairauksista, muistisairauksien varhaisesta tunnistamisesta sekä niiden tuomista haasteista ja vaikutuksista ihmisen jokapäiväiseen elämään. Tilaisuuteen osallistui yhteensä 140 henkilöä. Osallistujilta kerättiin kirjallinen palaute. Palautekyselyistä tehtiin yhteenveto ja kehittämisehdotukset kirjattiin myös opinnäytetyöhön. Tilaisuus vastasi yleisesti osallistujien odotuksia kiitettävästi. Projekti toteutettiin parityöskentelynä, jossa onnistuimme kiitettävästi.The age structure of Finnish population is changing rapidly within the next few decades. Statistics prognosis is that the number of over 75 years old people will be doubled by 2030, so the occurrence of memory disorders will increase with aging people. The idea for this study got its start from Senior Initiation project that wished a public lecture to be organized in Central Ostrobothnia for patients with memory disorder and their families. The purpose of our study was to organize a memory disorder event for the aforementioned groups. The occasion was open for anyone who was interested in it. The aim of this study was to provide current information to memory disorder patients and their loved ones about memory disorders and ways to cope with it at home as long as possible. Through our co-operation partners we surveyed the importance and need of the topic. Based on the feedback from their clients we were convinced of the importance of the event and the need for information. Our thesis was completed as a functional project work together with our client Senior Initiation project in Kokkola 4th of June 2015. The prevention of memory disorders, most common memory disorders, their early recognition and the challenges and effects they bring to people’s everyday lives were discussed during this event. The total of 140 people took part in the event. A written feedback was collected from all participants. A summary was made from the feedback survey, and all development proposals were registered also in this thesis. The event corresponded in general to the participants’ expectations very well. The project was implemented as pair work, in which we succeeded commendably

Tissue-specific regulation of the number of cell division rounds by inductive cell interaction and transcription factors during ascidian embryogenesis

AbstractMechanisms that regulate the number of cells constituting the body have remained largely elusive. We approached this issue in the ascidian, Halocynthia roretzi, which develops into a tadpole larva with a small number of cells. The embryonic cells divide 11 times on average from fertilization to hatching. The number of cell division rounds varies among tissue types. For example, notochord cells divide 9 times and give rise to large postmitotic cells in the tadpole. The number of cell division rounds in partial embryos derived from tissue-precursor blastomeres isolated at the 64-cell stage also varied between tissues and coincided with their counterparts in the intact whole embryos to some extent, suggesting tissue-autonomous regulation of cell division. Manipulation of cell fates in notochord, nerve cord, muscle, and mesenchyme lineage cells by inhibition or ectopic activation of the inductive FGF signal changed the number of cell divisions according to the altered fate. Knockdown and missexpression of Brachyury (Bra), an FGF-induced notochord-specific key transcription factor for notochord differentiation, indicated that Bra is also responsible for regulation of the number of cell division rounds, suggesting that Bra activates a putative mechanism to halt cell division at a specific stage. The outcome of precocious expression of Bra suggests that the mechanism involves a putative developmental clock that is likely shared in blastomeres other than those of notochord and functions to terminate cell division at three rounds after the 64-cell stage. Precocious expression of Bra has no effect on progression of the developmental clock itself

虚血性心疾患に対する体表面電位図の有用性: 運動負荷およびジピリダモ-ル負荷を用いた検討

取得学位 : 博士（医学）, 学位授与番号 : 医博甲第932号, 学位授与年月日：平成2年3月31日,学位授与年：199

Psychological Stress-Induced Oxidative Stress as a Model of Sub-Healthy Condition and the Effect of TCM

Distress-mediated tissue oxidative stress was examined as a model of sub-healthy condition defined in traditional Chinese medicine theory. Mice were subjected to psychologically stressful conditions by whiskers removal. Under this condition, spontaneous locomotive activity was significantly enhanced in the dark (P < 0.05 versus the control mice in three different movements), and granulocytes/lymphocytes balance shifted to granulocytes. At the same time, peroxynitrite level in blood plasma increased to ∼180% from that of the control mice at 6 h after removal of the whiskers (P < 0.01), and was maintained even after 12 h. Both protein carbonyl formation and lipid peroxidation were significantly increased under this condition in brain, heart, liver and spleen at 6 h after removal of whiskers (P < 0.05 or P < 0.01), and these levels were maximized after 12 h (increased to 120–160%, P < 0.05 or P < 0.01). The oxidative tissue injuries observed at 12 h after the removal of the whiskers were effectively prevented by two traditional Chinese medicine formula: Shengmai San (SMS) and Ling Gui Zhu Gan Tang (LGZGT), when administered for 5 days before the removal of the whiskers. Therefore, this stress model is considered useful in assessing the preventive potential of antioxidants and antioxidant-based herbal mixtures in treating the pathophysiology associated with psychological or emotional distress

イソニトリルの重合と金属錯体との反応に関する研究

Trace amounts of Pt- and Ru-doped Ni/Mg(Al)O catalysts were prepared by a citrate method and tested in the oxidative reforming of C3H8 under daily start-up and shut-down (DSS) operation. The activity and the sustainability of the catalysts were compared with those of the Pt- and Ru-doped Ni/Mg(Al)O catalysts derived from hydrotalcite (HT) precursor. The DSS operation of C3H8 reforming was carried Out with O-2 gas or O-2/H2O mixed gas between 200 degrees C and 600 degrees C or 700 degrees C under air purging conditions. The catalysts underwent steaming treatment with H-2/H2O mixed gas at 900 degrees C for 10 h. This allowed us to test the effect of Ni sintering on the catalyst deactivation. Coking was significantly suppressed on both HT- and citrate-derived Ni catalysts. Although both preparations produced highly dispersed Ni particles on the catalysts, the HT-derived catalysts exhibited more finely dispersed Ni particles, resulting in higher activity values than those of the citrate-derived catalysts, The regenerative activity due to redispersion of sintered Ni particles was enhanced over the HT-derived catalysts compared with the activity over citrate-derived catalysts. Although a clear redispersion of Ni particles was not observed in the oxidative reforming, i.e., in the absence of steam, the size decrease in Ni particles was more significant over the HT-derived catalysts than over the citrate-derived catalysts. The Mg(Al)O periclase structure derived from Mg-Al HT likely plays an important role in the regenerative activity of Pt- and Ru-Ni/Mg(Al)O catalysts. Pt-doping was more effective than Ru for the catalyst sustainability in the oxidative reforming of C3H8

Inhibition of ATR protein kinase activity by schisandrin B in DNA damage response

ATM and ATR protein kinases play a crucial role in cellular DNA damage responses. The inhibition of ATM and ATR can lead to the abolition of the function of cell cycle checkpoints. In this regard, it is expected that checkpoint inhibitors can serve as sensitizing agents for anti-cancer chemo/radiotherapy. Although several ATM inhibitors have been reported, there are no ATR-specific inhibitors currently available. Here, we report the inhibitory effect of schisandrin B (SchB), an active ingredient of Fructus schisandrae, on ATR activity in DNA damage response. SchB treatment significantly decreased the viability of A549 adenocarcinoma cells after UV exposure. Importantly, SchB treatment inhibited both the phosphorylation levels of ATM and ATR substrates, as well as the activity of the G2/M checkpoint in UV-exposed cells. The protein kinase activity of immunoaffinity-purified ATR was dose-dependently decreased by SchB in vitro (IC50: 7.25 μM), but the inhibitory effect was not observed in ATM, Chk1, PI3K, DNA-PK, and mTOR. The extent of UV-induced phosphorylation of p53 and Chk1 was markedly reduced by SchB in ATM-deficient but not siATR-treated cells. Taken together, our demonstration of the ability of SchB to inhibit ATR protein kinase activity following DNA damage in cells has clinical implications in anti-cancer therapy

Development of tumor-reactive T cells after nonmyeloablative allogeneic hematopoietic stem cell transplant for chronic lymphocytic leukemia.

PURPOSE: Allogeneic nonmyeloablative hematopoietic stem cell transplant (NM-HSCT) can result in durable remission of chronic lymphocytic leukemia (CLL). It is thought that the efficacy of NM-HSCT is mediated by recognition of tumor cells by T cells in the donor stem cell graft. We evaluated the development of CTLs specific for CLL after NM-HSCT to determine if their presence correlated with antitumor efficacy. EXPERIMENTAL DESIGN: Peripheral blood mononuclear cells obtained from 12 transplant recipients at intervals after NM-HSCT were stimulated in vitro with CLL cells. Polyclonal T-cell lines and CD8(+) T-cell clones were derived from these cultures and evaluated for lysis of donor and recipient target cells including CLL. The presence and specificity of responses was correlated with clinical outcomes. RESULTS: Eight of the 12 patients achieved remission or a major antitumor response and all 8 developed CD8(+) and CD4(+) T cells specific for antigens expressed by CLL. A clonal analysis of the CD8(+) T-cell response identified T cells specific for multiple minor histocompatibility (H) antigens expressed on CLL in six of the responding patients. A significant fraction of the CD8(+) T-cell response in some patients was also directed against nonshared tumor-specific antigens. By contrast, CLL-reactive T cells were not detected in the four patients who had persistent CLL after NM-HSCT, despite the development of graft-versus-host disease. CONCLUSIONS: The development of a diverse T-cell response specific for minor H and tumor-associated antigens expressed by CLL predicts an effective graft-versus-leukemia response after NM-HSCT

Numerical examination of plasmoid-induced reconnection model for solar flares: the relation between plasmoid velocity and reconnection rate

The plasmoid-induced-reconnection model explaining solar flares based on bursty reconnection produced by an ejecting plasmoid suggests a possible relation between the ejection velocity of a plasmoid and the rate of magnetic reconnection. In this study, we focus on the quantitative description of this relation. We performed magnetohydrodynamic (MHD) simulations of solar flares by changing the values of resistivity and the plasmoid velocity. The plasmoid velocity has been changed by applying an additional force to the plasmoid to see how the plasmoid velocity affects the reconnection rate. An important result is that the reconnection rate has a positive correlation with the plasmoid velocity, which is consistent with the plasmoid-induced-reconnection model for solar flares. We also discuss an observational result supporting this positive correlation.Comment: 27 pages, 12 figures, Accepted for publication in Ap

Spontaneous Celiac and Splenic Artery Dissection

Dissection o f t he splanchnic artery u nrelated to an aortic lesion is extremely rare. We describe a patient with dissection of the celiac and splenic arteries causing splenic circulatory impairment. A 55-year-old Japanese man was referred to our hospital for left back pain that suddenly occurred 3 days previously and spread to the left flank. He had complicated sleep apnea syndrome well controlled with continuous positive airway pressure, and had been prophylactically taking aspirin (100 mg/day) because of asymptomatic cerebral lacunar infarcts. Contrast-enhanced computed tomography (CT) in the arterial phase revealed dissection from the celiac root extending to the entire splenic artery, the caliber of which was irregularly narrowed, causing malperfusion in the spleen. Because of hemodynamic stability and lack of impending sequelae, the patient was carefully observed with rest, strict blood pressure control, and aspirin administration. One month later, CT revealed restoration of the caliber of the dissected arteries and regression of the organizing false lumen, which confirmed the patient’s recovery. Despite the extreme rarity or nonspecific symptoms, splanchnic artery dissection should be considered a potentially life-threatening emergency. This case supports the possible benefit of starting antithrombotic treatment early to prevent thrombotic sequelae such as organ infarction and aneurysmal formation

Properties of hadron screening masses at small baryonic density

The properties of hadron screening masses around the deconfinement phase transition at finite baryonic density can be studied by evaluating the Taylor coefficients with respect to the iso-scalar and iso-vector chemical potentials. We simulate 2-flavour lattice QCD with staggered fermions on a 12*12*24*6 lattice with ma = 0.05 and 0.10 and report investigations of nucleon, pseudo-scalar and vector mesons. We present new, strong evidence that in the confining phase, the screening masses at vanishing chemical potential have significant temperature dependence, but the effect of the iso-scalar chemical potential is very small. Above the critical temperature, the second derivative terms of mesons rapidly increase as contrasted to the case of baryon. We also study the responses of the screening masses to an iso-vector chemical potential and discuss some of the issues related to the properties of hadron masses at finite density.Comment: 7 pages with 5 figures; added two new references, the title of article and the section 'Concluding Remarks' are slightly change