196 research outputs found
Applications of signal transduction and xerophytophysiology by exposing hypocotyls in organic peanut production
The AnM practices in peanut production included three steps. The three letters, A, n and M, showed the shapes of the section-cross of the ridge at the three steps of different growth stages of the peanut crop. First, the peanut seeds were sown a little deeper than usual, about 8 cm, in the ridge to induce the extra-elongation of the hypocotyl. When the seeds were sown, the cross-section of the ridge looked like the letter “A”. The second, the hypocotyls elongated more than usual were exposed to light and dry air by removing the soil away around the young seedlings just after the emergence. At this time, the cross-section of the ridge looked like the letter “n”. The third, at the middle growth stages, soils on the both sides of ridge were earthed up to welcome the late pegs. At this time, the cross-section of the ridge looked like the letter “M”. Physiologically, the AnM technique induced osmotic adjustment, which improved photosynthetic activities by maintaining a higher leaf turgor potential. Anthocyanin accumulation was observed visually in hypocotyls of the young seedlings soon after the hypocotyl exposure started. The anthocyanin accumulation is accompanied by accumulations of soluble sugars, soluble proteins. All the consequences of the xerophytophysiological responses collaborated together to make the crop healthier through their individual function in plant growth and development. Gdi-15 (Groundnut desiccation induced) gene is a stress-responsive gene in peanut plant and its up-regulation expression was found in hypocotyl. In overall, hypocotyl exposure as a stimulation did induce the up-expression of the drought responsive gene, Gdi-15, and the consequent osmotic adjustment and anthocyanin accumulation but caused no damage to the whole plant. The AnM practice was more effective in the soil with compost applied to the surface layer and therefore it is feasible in organic peanut production
Arabidopsis heterotrimeric G protein β subunit interacts with a plasma membrane 2C-type protein phosphatase, PP2C52
AbstractHeterotrimeric G proteins (Gα, Gβ, Gγ) play important roles in signal transduction among various eukaryotic species. G proteins transmit signals by regulating the activities of effector proteins, but only a few Gβ-interacting effectors have been identified in plants. Here we show by a yeast two-hybrid screen that a putative myristoylated 2C-type protein phosphatase, PP2C52, is an Arabidopsis Gβ (AGB1)-interacting partner. The interaction between AGB1 and PP2C52 was confirmed by an in vitro pull-down assay and a bimolecular fluorescence complementation assay. PP2C52 transcripts were detected in many tissues. PP2C52 was localized to the plasma membrane and a mutation in the putative myristoylation site of PP2C52 disrupted its plasma membrane localization. Our results suggest that PP2C52 interacts with AGB1 on the plasma membrane and transmits signals via dephosphorylation of other proteins
Data-driven Exploration of New Pressure-induced Superconductivity in PbBiTe with Two Transition Temperatures
Candidates compounds for new thermoelectric and superconducting materials,
which have narrow band gap and flat bands near band edges, were exhaustively
searched by the high-throughput first-principles calculation from an inorganic
materials database named AtomWork. We focused on PbBiTe which has the
similar electronic band structure and the same crystal structure with those of
a pressure-induced superconductor SnBi2Se4 explored by the same data-driven
approach. The PbBiTe was successfully synthesized as single crystals
using a melt and slow cooling method. The core level X-ray photoelectron
spectroscopy analysis revealed Pb2+, Bi3+ and Te2- valence states in
PbBiTe. The thermoelectric properties of the PbBiTe sample were
measured at ambient pressure and the electrical resistivity was also evaluated
under high pressure using a diamond anvil cell with boron-doped diamond
electrodes. The resistivity decreased with increase of the pressure, and two
pressure-induced superconducting transitions were discovered at 3.4 K under
13.3 GPa and at 8.4 K under 21.7 GPa. The data-driven approach shows promising
power to accelerate the discovery of new thermoelectric and superconducting
materials
Soft X-ray Absorption and Photoemission Studies of Ferromagnetic Mn-Implanted 3-SiC
We have performed x-ray photoemission spectroscopy (XPS), x-ray absorption
spectroscopy (XAS), and resonant photoemission spectroscopy (RPES) measurements
of Mn-implanted 3-SiC (3-SiC:Mn) and carbon-incorporated MnSi
(MnSi:C). The Mn 2 core-level XPS and XAS spectra of 3-SiC:Mn
and MnSi:C were similar to each other and showed "intermediate"
behaviors between the localized and itinerant Mn 3 states.
The intensity at the Fermi level was found to be suppressed in 3-SiC:Mn
compared with MnSi:C. These observations are consistent with the
formation of MnSi:C clusters in the 3-SiC host, as observed in a
recent transmission electron microscopy study.Comment: 4 pages, 3 figure
Comprehensive Analysis of NAC Transcription Factor Family Uncovers Drought and Salinity Stress Response in Pearl Millet (\u3cem\u3ePennisetum glaucum\u3c/em\u3e)
BACKGROUND: Pearl millet (Pennisetum glaucum) is a cereal crop that possesses the ability to withstand drought, salinity and high temperature stresses. The NAC [NAM (No Apical Meristem), ATAF1 (Arabidopsis thaliana Activation Factor 1), and CUC2 (Cup-shaped Cotyledon)] transcription factor family is one of the largest transcription factor families in plants. NAC family members are known to regulate plant growth and abiotic stress response. Currently, no reports are available on the functions of the NAC family in pearl millet.
RESULTS: Our genome-wide analysis found 151 NAC transcription factor genes (PgNACs) in the pearl millet genome. Thirty-eight and 76 PgNACs were found to be segmental and dispersed duplicated respectively. Phylogenetic analysis divided these NAC transcription factors into 11 groups (A-K). Three PgNACs (− 073, − 29, and − 151) were found to be membrane-associated transcription factors. Seventeen other conserved motifs were found in PgNACs. Based on the similarity of PgNACs to NAC proteins in other species, the functions of PgNACs were predicted. In total, 88 microRNA target sites were predicted in 59 PgNACs. A previously performed transcriptome analysis suggests that the expression of 30 and 42 PgNACs are affected by salinity stress and drought stress, respectively. The expression of 36 randomly selected PgNACs were examined by quantitative reverse transcription-PCR. Many of these genes showed diverse salt- and drought-responsive expression patterns in roots and leaves. These results confirm that PgNACs are potentially involved in regulating abiotic stress tolerance in pearl millet.
CONCLUSION: The pearl millet genome contains 151 NAC transcription factor genes that can be classified into 11 groups. Many of these genes are either upregulated or downregulated by either salinity or drought stress and may therefore contribute to establishing stress tolerance in pearl millet
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