As ações da escola frente ao fracasso escolar

Monografia (graduação)—Universidade de Brasília, Faculdade de Educação, 2015.O presente trabalho tem por objetivo geral analisar as ações da escola frente ao fracasso escolar através da visão de cinco professores da rede pública de ensino do Distrito Federal. Através referencial teórico a cerca do fracasso escolar foi elaborado com base nos estudos de Patto (2010) e Bossa (2012). A pesquisa tem uma metodologia de caráter qualitativo. Para a coleta dos dados o instrumento utilizado foi um questionário com seis perguntas encaminhado a cinco docentes da Secretaria de Estado e Educação do Distrito Federal. Com a realização da pesquisa pude perceber que a escola por mais que tenha algumas estratégias para o combate ao fracasso escolar, ainda deixa muito a desejar, que os professores estão descontentes com suas condições de trabalho e que os alunos são estereotipados e predestinados ao fracasso por suas condições sociais

Composição Florística do Componente Herbáceo do Jardim Botânico da UFSM, Santa Maria, Rio Grande do Sul

The district of Santa Maria is located in the northern region of theCentral Depression of Rio Grande do Sul and its vegetation is characterized by floras of phytoecological regions such as Savanna and Seasonal Forest.The Campus of the Federal University of Santa Maria (UFSM) has a totalarea of 1906.57 hectares, including the Botanical Garden (JB-UFSM), whichhas an area of approximately 14.5 hectares and it is located between thecoordinates 29°43’22'’S and 53°43’47'’W. Its vegetation has changed andbecame quite different from the characteristic vegetation of the Santa Mariaregion, mainly as a result of farming and the introduction of plant species.This work aimed to perform a floristic study of the herbaceous componentof the UFSM Botanical Garden, providing data for help in the cultivation,in the species conservation and the recovery of the area. Specimens werecollected seasonally, from March 2006 to March 2007, carried out duringwalks along the study area. The species were collected during the fertilestage and taken to the laboratory of Plant Taxonomy for identification andherborization and then deposited in the SMDB Herbarium. A total of 201species in 128 genera and 34 families were identified, with Poaceae,Asteraceae, Fabaceae and Cyperaceae being the most representative families.The data obtained with the identification of the material was used to drawup a list of all species, as well as an analytical key to the families.Este trabalho realizou um estudo florístico do componente herbáceo do JB-UFSM, visando a fornecer dados para serem utilizados no cultivo, na conservação das espécies e na recuperação da área

Fully coupled hydrological–hydrodynamic modeling of a basin–river–lake transboundary system in Southern South America

The Mirim and Patos Lagoons form the largest lagoon complex in South America. Wind is one of the dominant climatic elements of circulation and water levels in the basin. Therefore, we aimed to better understand the effects of wind on the Mirim–São Gonçalo watershed by applying the MGB hydrological model and to assess whether it would produce satisfactory results for modeling. Various tests were performed to determine the best representation of the processes involved and the observed levels. The best results were obtained with the inclusion of sub-daily wind data in the simulation and also the downstream boundary condition by using the observed water level data at the sluice dam of the São Gonçalo channel. The results showed that the model could successfully simulate the levels and demonstrated the importance of including the wind when modeling the hydrodynamic processes of large lake environments

Bloqueios locorregionais para correção de fenda palatina em potro: Relato de caso

O presente trabalho tem como objetivo descrever o caso de um potro da raça Quarto de Milha, com 9 meses de idade e pesando 166 kg, atendido pelo setor de clínica médica e cirúrgica de grandes animais do Hospital Veterinário da Universidade Federal do Paraná para correção de uma fenda palatina. Como medicação pré-anestésica, fez-se o uso de xilazina 0,5 mg/kg. Posteriormente, a indução foi realizada com propofol 1mg/kg e cetamina 1 mg/kg. Para manutenção anestésica, optou-se pela utilização de isoflurano, lidocaína (3 mg/kg/h), cetamina (0,6 mg/kg/h) e dexmedetomidina (1 ug/kg/h). A analgesia transoperatória foi somada com a realização de bloqueios locorregionais, sendo executados por referências anatômicas os bloqueios dos nervos maxilar, mentual e alveolar inferior com lidocaína e bupivacaína, utilizando 3 ml por ponto. Dentre os parâmetros fisiológicos monitorados, destaca-se a frequência cardíaca, pressão arterial sistólica, pressão arterial média e pressão arterial diastólica, as quais mantiveram-se estáveis durante todo o período anestésico. Por fim, a técnica de bloqueio dos nervos maxilar, mentual e alveolar inferior mostraram-se alternativas viáveis para o procedimento proposto, uma vez que foram inexistentes as alterações paramétricas de nocicepção no transoperatório

Effect of Foot Reflexology on Muscle Electrical Activity, Pressure, Plantar Distribution, and Body Sway in Patients with Type 2 Diabetes Mellitus: A Pilot Randomized Controlled Trial

Objective: To verify the effect of foot reflexology on the electrical muscle activity of the lateral and medial gastrocnemius muscle, and to examine the distribution, plantar pressure, and body sway in patients with type 2 diabetes mellitus. Methods: This pilot randomized controlled trial enrolled 17 volunteers who were clinically diagnosed with diabetes mellitus. The sample was assigned to one of two groups: the control group (CG, n = 7), who received information on foot care and health, and the intervention group (IG, n = 10), who received the application of foot reflexology on specific areas of the feet, for 10 consecutive days. There was blinding of the evaluator and the therapist. Surface electromyography (EMG) was used to assess the electrical activity of the medial and lateral gastrocnemius muscles in maximum voluntary isometric contraction (MVIC) and isotonic contraction (IC); baropodometry and stabilometry were used to analyze unloading, plantar weight distribution, and body sway. Results: There was a statistically significant difference for the variables of maximum peak electrical activity of the left medial gastrocnemius (p = 0.03; effect size = 0.87 and power = 0.81) and left lateral gastrocnemius muscles (p = 0.04, effect size = 0.70 and power = 0.66) respectively, in the intragroup IC, and median frequency of the left medial gastrocnemius muscle in the intragroup MVIC (p = 0.03; effect size = 0.64 and power = 0.59), and in the variables intergroups of the total area on the right side (p = 0.04; effect size = 1.03 and power = 0.50) and forefoot area on the left side (p = 0.02; effect size = 0.51 and power = 0.16). Conclusions: We conclude that foot reflexology influenced some variables of the intergroup plantar distribution and intragroup EMG in the sample studied. There is a need for a placebo group, a larger sample and a follow-up to strengthen the findings of these experiments

Maternal 5mCpG Imprints at the PARD6G-AS1 and GCSAML Differentially Methylated Regions Are Decoupled From Parent-of-Origin Expression Effects in Multiple Human Tissues

A hallmark of imprinted genes in mammals is the occurrence of parent-of-origin-dependent asymmetry of DNA cytosine methylation (5mC) of alleles at CpG islands (CGIs) in their promoter regions. This 5mCpG asymmetry between the parental alleles creates allele-specific imprinted differentially methylated regions (iDMRs). iDMRs are often coupled to the transcriptional repression of the methylated allele and the activation of the unmethylated allele in a tissue-specific, developmental-stage-specific and/or isoform-specific fashion. iDMRs function as regulatory platforms, built through the recruitment of chemical modifications to histones to achieve differential, parent-of-origin-dependent chromatin segmentation states. Here, we used a comparative computational data mining approach to identify 125 novel constitutive candidate iDMRs that integrate the maximal number of allele-specific methylation region records overlapping CGIs in human methylomes. Twenty-nine candidate iDMRs display gametic 5mCpG asymmetry, and another 96 are candidate secondary iDMRs. We established the maternal origin of the 5mCpG imprints of one gametic (PARD6G-AS1) and one secondary (GCSAML) iDMRs. We also found a constitutively hemimethylated, nonimprinted domain at the PWWP2AP1 promoter CGI with oocyte-derived methylation asymmetry. Given that the 5mCpG level at the iDMRs is not a sufficient criterion to predict active or silent locus states and that iDMRs can regulate genes from a distance of more than 1 Mb, we used RNA-Seq experiments from the Genotype-Tissue Expression project and public archives to assess the transcriptional expression profiles of SNPs across 4.6 Mb spans around the novel maternal iDMRs. We showed that PARD6G-AS1 and GCSAML are expressed biallelically in multiple tissues. We found evidence of tissue-specific monoallelic expression of ZNF124 and OR2L13, located 363 kb upstream and 419 kb downstream, respectively, of the GCSAML iDMR. We hypothesize that the GCSAML iDMR regulates the tissue-specific, monoallelic expression of ZNF124 but not of OR2L13. We annotated the non-coding epigenomic marks in the two maternal iDMRs using data from the Roadmap Epigenomics project and showed that the PARD6G-AS1 and GCSAML iDMRs achieve contrasting activation and repression chromatin segmentations. Lastly, we found that the maternal 5mCpG imprints are perturbed in several hematopoietic cancers. We conclude that the maternal 5mCpG imprints at PARD6G-AS1 and GCSAML iDMRs are decoupled from parent-of-origin transcriptional expression effects in multiple tissues

Image7.TIF

<p>A hallmark of imprinted genes in mammals is the occurrence of parent-of-origin-dependent asymmetry of DNA cytosine methylation (5^mC) of alleles at CpG islands (CGIs) in their promoter regions. This 5^mCpG asymmetry between the parental alleles creates allele-specific imprinted differentially methylated regions (iDMRs). iDMRs are often coupled to the transcriptional repression of the methylated allele and the activation of the unmethylated allele in a tissue-specific, developmental-stage-specific and/or isoform-specific fashion. iDMRs function as regulatory platforms, built through the recruitment of chemical modifications to histones to achieve differential, parent-of-origin-dependent chromatin segmentation states. Here, we used a comparative computational data mining approach to identify 125 novel constitutive candidate iDMRs that integrate the maximal number of allele-specific methylation region records overlapping CGIs in human methylomes. Twenty-nine candidate iDMRs display gametic 5^mCpG asymmetry, and another 96 are candidate secondary iDMRs. We established the maternal origin of the 5^mCpG imprints of one gametic (PARD6G-AS1) and one secondary (GCSAML) iDMRs. We also found a constitutively hemimethylated, nonimprinted domain at the PWWP2AP1 promoter CGI with oocyte-derived methylation asymmetry. Given that the 5^mCpG level at the iDMRs is not a sufficient criterion to predict active or silent locus states and that iDMRs can regulate genes from a distance of more than 1 Mb, we used RNA-Seq experiments from the Genotype-Tissue Expression project and public archives to assess the transcriptional expression profiles of SNPs across 4.6 Mb spans around the novel maternal iDMRs. We showed that PARD6G-AS1 and GCSAML are expressed biallelically in multiple tissues. We found evidence of tissue-specific monoallelic expression of ZNF124 and OR2L13, located 363 kb upstream and 419 kb downstream, respectively, of the GCSAML iDMR. We hypothesize that the GCSAML iDMR regulates the tissue-specific, monoallelic expression of ZNF124 but not of OR2L13. We annotated the non-coding epigenomic marks in the two maternal iDMRs using data from the Roadmap Epigenomics project and showed that the PARD6G-AS1 and GCSAML iDMRs achieve contrasting activation and repression chromatin segmentations. Lastly, we found that the maternal 5^mCpG imprints are perturbed in several hematopoietic cancers. We conclude that the maternal 5^mCpG imprints at PARD6G-AS1 and GCSAML iDMRs are decoupled from parent-of-origin transcriptional expression effects in multiple tissues.</p

Image3.TIF

<p>A hallmark of imprinted genes in mammals is the occurrence of parent-of-origin-dependent asymmetry of DNA cytosine methylation (5^mC) of alleles at CpG islands (CGIs) in their promoter regions. This 5^mCpG asymmetry between the parental alleles creates allele-specific imprinted differentially methylated regions (iDMRs). iDMRs are often coupled to the transcriptional repression of the methylated allele and the activation of the unmethylated allele in a tissue-specific, developmental-stage-specific and/or isoform-specific fashion. iDMRs function as regulatory platforms, built through the recruitment of chemical modifications to histones to achieve differential, parent-of-origin-dependent chromatin segmentation states. Here, we used a comparative computational data mining approach to identify 125 novel constitutive candidate iDMRs that integrate the maximal number of allele-specific methylation region records overlapping CGIs in human methylomes. Twenty-nine candidate iDMRs display gametic 5^mCpG asymmetry, and another 96 are candidate secondary iDMRs. We established the maternal origin of the 5^mCpG imprints of one gametic (PARD6G-AS1) and one secondary (GCSAML) iDMRs. We also found a constitutively hemimethylated, nonimprinted domain at the PWWP2AP1 promoter CGI with oocyte-derived methylation asymmetry. Given that the 5^mCpG level at the iDMRs is not a sufficient criterion to predict active or silent locus states and that iDMRs can regulate genes from a distance of more than 1 Mb, we used RNA-Seq experiments from the Genotype-Tissue Expression project and public archives to assess the transcriptional expression profiles of SNPs across 4.6 Mb spans around the novel maternal iDMRs. We showed that PARD6G-AS1 and GCSAML are expressed biallelically in multiple tissues. We found evidence of tissue-specific monoallelic expression of ZNF124 and OR2L13, located 363 kb upstream and 419 kb downstream, respectively, of the GCSAML iDMR. We hypothesize that the GCSAML iDMR regulates the tissue-specific, monoallelic expression of ZNF124 but not of OR2L13. We annotated the non-coding epigenomic marks in the two maternal iDMRs using data from the Roadmap Epigenomics project and showed that the PARD6G-AS1 and GCSAML iDMRs achieve contrasting activation and repression chromatin segmentations. Lastly, we found that the maternal 5^mCpG imprints are perturbed in several hematopoietic cancers. We conclude that the maternal 5^mCpG imprints at PARD6G-AS1 and GCSAML iDMRs are decoupled from parent-of-origin transcriptional expression effects in multiple tissues.</p

Image1.TIF

<p>A hallmark of imprinted genes in mammals is the occurrence of parent-of-origin-dependent asymmetry of DNA cytosine methylation (5^mC) of alleles at CpG islands (CGIs) in their promoter regions. This 5^mCpG asymmetry between the parental alleles creates allele-specific imprinted differentially methylated regions (iDMRs). iDMRs are often coupled to the transcriptional repression of the methylated allele and the activation of the unmethylated allele in a tissue-specific, developmental-stage-specific and/or isoform-specific fashion. iDMRs function as regulatory platforms, built through the recruitment of chemical modifications to histones to achieve differential, parent-of-origin-dependent chromatin segmentation states. Here, we used a comparative computational data mining approach to identify 125 novel constitutive candidate iDMRs that integrate the maximal number of allele-specific methylation region records overlapping CGIs in human methylomes. Twenty-nine candidate iDMRs display gametic 5^mCpG asymmetry, and another 96 are candidate secondary iDMRs. We established the maternal origin of the 5^mCpG imprints of one gametic (PARD6G-AS1) and one secondary (GCSAML) iDMRs. We also found a constitutively hemimethylated, nonimprinted domain at the PWWP2AP1 promoter CGI with oocyte-derived methylation asymmetry. Given that the 5^mCpG level at the iDMRs is not a sufficient criterion to predict active or silent locus states and that iDMRs can regulate genes from a distance of more than 1 Mb, we used RNA-Seq experiments from the Genotype-Tissue Expression project and public archives to assess the transcriptional expression profiles of SNPs across 4.6 Mb spans around the novel maternal iDMRs. We showed that PARD6G-AS1 and GCSAML are expressed biallelically in multiple tissues. We found evidence of tissue-specific monoallelic expression of ZNF124 and OR2L13, located 363 kb upstream and 419 kb downstream, respectively, of the GCSAML iDMR. We hypothesize that the GCSAML iDMR regulates the tissue-specific, monoallelic expression of ZNF124 but not of OR2L13. We annotated the non-coding epigenomic marks in the two maternal iDMRs using data from the Roadmap Epigenomics project and showed that the PARD6G-AS1 and GCSAML iDMRs achieve contrasting activation and repression chromatin segmentations. Lastly, we found that the maternal 5^mCpG imprints are perturbed in several hematopoietic cancers. We conclude that the maternal 5^mCpG imprints at PARD6G-AS1 and GCSAML iDMRs are decoupled from parent-of-origin transcriptional expression effects in multiple tissues.</p

DataSheet2.XLSX

<p>A hallmark of imprinted genes in mammals is the occurrence of parent-of-origin-dependent asymmetry of DNA cytosine methylation (5^mC) of alleles at CpG islands (CGIs) in their promoter regions. This 5^mCpG asymmetry between the parental alleles creates allele-specific imprinted differentially methylated regions (iDMRs). iDMRs are often coupled to the transcriptional repression of the methylated allele and the activation of the unmethylated allele in a tissue-specific, developmental-stage-specific and/or isoform-specific fashion. iDMRs function as regulatory platforms, built through the recruitment of chemical modifications to histones to achieve differential, parent-of-origin-dependent chromatin segmentation states. Here, we used a comparative computational data mining approach to identify 125 novel constitutive candidate iDMRs that integrate the maximal number of allele-specific methylation region records overlapping CGIs in human methylomes. Twenty-nine candidate iDMRs display gametic 5^mCpG asymmetry, and another 96 are candidate secondary iDMRs. We established the maternal origin of the 5^mCpG imprints of one gametic (PARD6G-AS1) and one secondary (GCSAML) iDMRs. We also found a constitutively hemimethylated, nonimprinted domain at the PWWP2AP1 promoter CGI with oocyte-derived methylation asymmetry. Given that the 5^mCpG level at the iDMRs is not a sufficient criterion to predict active or silent locus states and that iDMRs can regulate genes from a distance of more than 1 Mb, we used RNA-Seq experiments from the Genotype-Tissue Expression project and public archives to assess the transcriptional expression profiles of SNPs across 4.6 Mb spans around the novel maternal iDMRs. We showed that PARD6G-AS1 and GCSAML are expressed biallelically in multiple tissues. We found evidence of tissue-specific monoallelic expression of ZNF124 and OR2L13, located 363 kb upstream and 419 kb downstream, respectively, of the GCSAML iDMR. We hypothesize that the GCSAML iDMR regulates the tissue-specific, monoallelic expression of ZNF124 but not of OR2L13. We annotated the non-coding epigenomic marks in the two maternal iDMRs using data from the Roadmap Epigenomics project and showed that the PARD6G-AS1 and GCSAML iDMRs achieve contrasting activation and repression chromatin segmentations. Lastly, we found that the maternal 5^mCpG imprints are perturbed in several hematopoietic cancers. We conclude that the maternal 5^mCpG imprints at PARD6G-AS1 and GCSAML iDMRs are decoupled from parent-of-origin transcriptional expression effects in multiple tissues.</p