The quantitative proteomic response of Synechocystis sp. PCC6803 to phosphate acclimation.

BACKGROUND: Inorganic phosphate (Pi) is a critical nutrient for all life and is periodically limiting in marine and freshwater provinces, yet little is understood how organisms acclimate to fluctuations in Pi within their environment. To investigate whole cell adaptation, we grew Synechocystis sp. PCC6803, a model freshwater cyanobacterium, in 3%, and 0.3% inorganic phosphate (Pi) media. The cells were allowed to acclimate over 60 days, and cells were harvested for quantitative high throughput mass spectrometry-based proteomics using the iTRAQ™ labelling technology. RESULTS: In total, 120 proteins were identified, and 52 proteins were considered differentially abundant compared to the control. Alkaline phosphatase (APase) activities correlated significantly (p < 0.05) with observed relative PhoA abundances. PstS1 and PstS2 were both observed, yet PstS1 was not differentially more abundant than the control. Phycobilisome protein abundances appeared to be coordinated, and are significantly less abundant in 0.3% Pi than 3% Pi cultures. Also, the central metabolic cell function appears to have shifted towards the production of (NADPH) reducing energy and nucleotide sugars. CONCLUSIONS: This acclimation response bears strong similarity to the previously reported response to nitrogen deprivation within Synechocystis sp. PCC 6803. However, it also demonstrates some characteristics of desiccation stress, such as the regulation of fatty acids and increased abundance of rehydrin in the 3% Pi culture

Biodegradation of aminoalkylphosphonates by environmental bacteria

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Assessment of low-cost cartridge filters for implementation in household drinking water treatment systems

In regions where houses are sparsely located, traditional centralized water treatment plants are not economically feasible, with household water treatment (HWT) systems commonly used to provide potable water for a range of household activities. Filtration prior to disinfection is essential, and due to their ease of use and small footprint, cartridge filters are commonly employed. In this work, readily available commercial filter types (spun, wound and pleated) of different micron ratings (10, 5 and 1) were tested for the removal of turbidity either alone or in series via simulated large volume pilot trials. Water with an initial turbidity of 40 ± 10 NTU was prepared using fine test dust (ISO 12103-1, A2) with the turbidity removal efficiency, pressure drop, total capacity and lifespan of the filters evaluated. To increase useable filter lifetime upon reaching the 1 bar pressure limit, a series of washing steps were employed to regenerate the filters. Whilst pleated filters could be efficiently cleaned, spun and wound filters could not, and were discarded after single use. In pilot trials, the volume of turbid water filtered varied from 0.85 m3 with a 1 micron wound filter to 6 m3, with 5 and 1 micron pleated filters in series, which following regeneration could be used for three filtration cycles. For pleated filters, turbidity removal efficiency improved over time as a cake built up resulting in the effluent turbidity reaching acceptable quality (&lt;5 NTU). This criterion continued to be achieved with repeated cycles of washed pleated filters, thereby significantly reducing the cost and improving sustainability of the HWT system. Field trials were carried out with a similar HWT system (5 and 1 micron spun filters) installed in households of rural communities in Curiti, Colombia. Turbidity was effectively removed from natural water (reduction to &lt;1.2 NTU) with improved efficacy in comparison to synthetic water samples due to the large particle size distribution observed in the natural water. © 2020 Elsevier Lt