Investigation on anti-proliferation properties of porcupine bezoar (hystrix brachyuran) extracts exposed on hela cells lines combined with electroporation technique

Electroporation (EP) is a technique whereby the biophysical changes on the cells that induced external high-intensity electrical field pulses in order to enhance applications in the medical field. It is a molecular biology technique in order to create pores through a cell wall membrane, boost the permeability of the cell membrane, and support chemicals, drugs or DNA to be imported into Hela cells. While by combining electroporation (EP) technique with porcupine bezoar (PB) extract might reduce the proliferation of HeLa cells because this compound extract has the ability of anti-proliferation and also anti-angiogenesis properties for controlling cancer cell growth. This research concentrate on reviewing and analyses the basic concepts and methods of combining electroporation and porcupine bezoar (PB) extract as applied in cancer treatment application. The combination of this technique might be a new alternative for anti-cancer treatment. The combination of this technique might be a new way for anti-cancer treatment. © 2018 Universiti Teknikal Malaysia Melaka. All rights reserved

IoT based dual axis solar tracker implementation for polycrystalline photovoltaic with energy storage

This project has been identified as IoT Based Dual Axis Solar Tracker Implementation for Polycrystalline Photovoltaic with Energy Storage that has two degrees of freedom that act as axes of rotation, both horizontal and vertical. Polycrystalline panel is great in both diffuse light and specular light and yet known for its minimal cost. This motorized servo-motor system based final year project is a self-orient the solar panel towards the maximum intensity of sunlight using Light Dependent Resistor (LDR) and hence, it will generate supply to the load. The main system that use to control the movement of motor and sense the LDR is Arduino Uno. This portable 10W solar project can be used anywhere at any time as its main concern to educate people of basic solar tracker concept aside to help supplying 5V Direct Current (DC) load. An Internet of Things (IoT) is therefore added as a feature for this project to monitor the performance of photovoltaic voltage through Blynk application in cell phon

Neonatal health monitoring system with IOT application

The body temperature and heart rate are important parameters to monitor the neonatal situation. Neonatal need to be monitored closely in Neonatal Intensive Care Unit (NICU) to allow quick actions by physicians when any problems occur. The lack of systems that able to alert and indicates any warning condition of neonatal in NICU is one of the problems faced by most of the hospital. To reduce the complexity of the system at the NICU for monitoring the Neonatal condition from time to time, the health monitoring system using the Android app has been introduced. This system was introduced to improve the existing system to give alert and warning sign so that early precautions can be done. The project involves the LM35 temperature sensor and pulse sensor which is controlled by the Arduino Uno microcontroller with the help of instructions C / C ++, and Bluetooth networking system. This allows the user to monitor the baby's condition through applications that use Bluetooth networking system. Users must have the application that can be downloaded on Google Play. Furthermore, this system is not only applicable for the physicians but also for others to monitor the condition of Neonatal even though they are outside of the NICU. The neonatal condition can be checked by the LCD display

Investigation of porcupine bezoar extract combined with electroporation on HeLa cell

Current chemo-preventive agents will causes a long term side effects in cancer sufferer. Therefore, this research focusses on the benefits of combine both technique of Electroporation (EP) method and with natural animal extract in process to inhibit the proliferation of cancer cell, as a solution to reduce or adverse effects of orthodox drugs. The present study indicates on anti-cancer potentially of Porcupine Bezoar Extract (PBE), also known as Hystrix Brachyuran against HeLa cell. Cell viability of HeLa cell were determined after HeLa cell treated with 500 V/cm and pulse duration of 100µs before the concentration of 80.0 µg/ml of Porcupine Bezoar Extract (PBE) was added into the cell. The cell viability and cell growth were monitored up to 48 hours with comparative to untreated cell as a control group.This study result proved that by combine both technique; Electroporation (EP) and with natural animal extract Porcupine Bezoar Extract (PBE) might open the door and it has an ability in supressing the growth of HeLa cell

In-vitro evaluation of porcupine bezoar extracts (PBE) as anti-cancer agent on HeLa cell line – A preliminary study

Porcupine Bezoar Extract (PBE) has been previously described to possess diverse medicinal properties. In any case, its potential and ability as anti-cancer agent on HeLa cell line are still poorly studied and understood. Hence, in this research, Porcupine Bezoar Extract (PBE) aqueous extract was figure out for its potential as a safe anti-cancer agent. Initially, Porcupine Bezoar Extract (PBE) was infused with distilled water for 18 hours to get PBE aqueous solution in order to confirm the presence of bioactive agents as antioxidants. Later, PBE aqueous solution was subjected to HeLa cell line and was observed for 48 hours exposures with comparative to the untreated cell to evaluate its anticancer potential and ability. The data from this study propose the potential agents for improving human health, especially in the anti-cancer application

Studying the Influence of Electroporation on HT29 Cell Line Interaction with Fibronectin and Collagen Protein Micro-Patterned Surface

Micro-contact printing (MCP) is a scheme that allows a substrate or surface to be functionalized freely with extracellular matrix (ECM) protein such as fibronectin and collagen, in a well-defined manner. MCP can be used to regulate cell adhesion geometry on a substrate and in controlling wound healing process by facilitating directed cell migration. In this study, human colon cancer cell line, HT29 were grown on a micro-contact printed pattern of fibronectin and collagen protein with repeat gratings of 25μm, 50μm, and 100μm wide, for 48 hours. The cells alignments to the patterned substrates were then computed, where 0° means 100% alignment to the pattern. This was done with the purpose of finding those pattern that stimulated the best degree of cell alignment. Best alignment and elongation were obtained on 50μm of the two ECM proteins. The quantitative analysis of the results revealed that HT29 cells aligned most readily to the 50μm width pattern with a mean angle of alignment of 5.0-1.3 and 16.1-4.6, respectively, on fibronectin and collagen pattern surfaces. Contrarily, the cells aligned poorly on the 25μm width pattern of fibronectin, collagen and the control substrates with a mean angle of 33.4-8.4, 36.2-8.9 and 54.5-6.0, respectively. Furthermore, the 50μm stamp pattern was used to investigate the influence of pulse electric field (PEF) on the HT29 alignment to the patterned substrate. The result revealed that there was significant improvement (P < 0.05) in the cell alignment between the electrically treated and the untreated cells. The alignment angles of the electrically treated cells were 4.0-1.2 and 11.2-3.5, respectively, on the 50μm pattern surface of fibronectin and collagen. Therefore, the result of the study revealed that micro-contact printing technique together with pulse electric field could offer a potentially fast method of controlling directed cell migration for wound healing application