The Structure of the NPC1L1 N-Terminal Domain in a Closed Conformation

NPC1L1 is the molecular target of the cholesterol lowering drug Ezetimibe and mediates the intestinal absorption of cholesterol. Inhibition or deletion of NPC1L1 reduces intestinal cholesterol absorption, resulting in reduction of plasma cholesterol levels.Here we present the 2.8 Å crystal structure of the N-terminal domain (NTD) of NPC1L1 in the absence of cholesterol. The structure, combined with biochemical data, reveals the mechanism of cholesterol selectivity of NPC1L1. Comparison to the cholesterol free and bound structures of NPC1(NTD) reveals that NPC1L1(NTD) is in a closed conformation and the sterol binding pocket is occluded from solvent.The structure of NPC1L1(NTD) reveals a degree of flexibility surrounding the entrance to the sterol binding pocket, suggesting a gating mechanism that relies on multiple movements around the entrance to the sterol binding pocket

Cultural adaptation and validation of an instrument on barriers for the use of research results

ABSTRACT Objective: to culturally adapt The Barriers to Research Utilization Scale and to analyze the metric validity and reliability properties of its Brazilian Portuguese version. Method: methodological research conducted by means of the cultural adaptation process (translation and back-translation), face and content validity, construct validity (dimensionality and known groups) and reliability analysis (internal consistency and test-retest). The sample consisted of 335 nurses, of whom 43 participated in the retest phase. Results: the validity of the adapted version of the instrument was confirmed. The scale investigates the barriers for the use of the research results in clinical practice. Confirmatory factorial analysis demonstrated that the Brazilian Portuguese version of the instrument is adequately adjusted to the dimensional structure the scale authors originally proposed. Statistically significant differences were observed among the nurses holding a Master's or Doctoral degree, with characteristics favorable to Evidence-Based Practice, and working at an institution with an organizational cultural that targets this approach. The reliability showed a strong correlation (r ranging between 0.77 and 0.84, p<0.001) and the internal consistency was adequate (Cronbach's alpha ranging between 0.77 and 0.82). Conclusion: the Brazilian Portuguese version of The Barriers Scale was valid and reliable in the group studied

satisfaction of recently married couples

Background: Through its ability to address and remove fear and misunderstanding and the resulting sexual reluctance and related problems, pre-marital sexual education and counselling can contribute to sexual satisfaction. Methods: This quasi-experimental research conducted in a pre-test-post-test control group design aimed to examine the effectiveness of nursing interventions on a premarital counselling program and its impacts on the sexual satisfaction of couples (36 couples in the experimental group and 35 couples in the control group). Results: Although no difference was detected between the experimental and control groups in terms of the level of knowledge on pre-test point averages, the difference between them in terms of post-test knowledge gain averages was statistically significant. Approval rates for sexual myths in the pre-test were 27.87% in the experimental group and 37.03% in the control group; in the post-test they were 23.51% and 36.66% respectively. In the experimental group, 80.6% of the women and 63.9% of the men, and in the control group, 77.1% of the women and 71.4% of the men were established as having a problem-free sexual life. Conclusions: It was also discovered that levels of sexual satisfaction were shown to be higher among women and men in the experimental group who had attended premarital sexual counselling education than the women and men in the control group. A recommendation to encourage engaged couples to attend premarital sexual counselling is made based on the findings. It is thought that an intervention plan prepared within the framework of the Information, Motivation, Behavioural Skills theoretical model will help nurses guide recently-married couples to greater sexual satisfaction

The effect of the support program on the resilience of female family caregivers of stroke patients: Randomized controlled trial.

AIM: The purpose of the study was to determine the effect of a support program on the resilience of female family caregivers of stroke patients. METHODS: This is a randomized controlled trial. The sample consisted 70 female family caregivers (34 experimental, 36 control group). Data were collected three times (pretest-posttest, follow-up test). Data were collected using the demographical data form, the Family Index of Regenerativity and Adaptation-General. RESULTS: A significant difference was determined between the experimental and control group's follow-up test scores for relative and friend support, social support and family-coping coherence. A significant difference was determined between the experimental group's mean pretest, posttest and follow-up test scores in terms of family strain, relative and friend support, social support, family coping-coherence, family hardiness and family distress. CONCLUSIONS: These results suggest that the Support Program contributes to the improvement of the components of resilience of family caregivers of stroke patients