The 5th edition of the World Health Organization classification of haematolymphoid tumours: myeloid and histiocytic/dendritic neoplasms

The upcoming 5th edition of the World Health Organization (WHO) Classification of Haematolymphoid Tumours is part of an effort to hierarchically catalogue human cancers arising in various organ systems within a single relational database. This paper summarizes the new WHO classification scheme for myeloid and histiocytic/dendritic neoplasms and provides an overview of the principles and rationale underpinning changes from the prior edition. The definition and diagnosis of disease types continues to be based on multiple clinicopathologic parameters, but with refinement of diagnostic criteria and emphasis on therapeutically and/or prognostically actionable biomarkers. While a genetic basis for defining diseases is sought where possible, the classification strives to keep practical worldwide applicability in perspective. The result is an enhanced, contemporary, evidence-based classification of myeloid and histiocytic/dendritic neoplasms, rooted in molecular biology and an organizational structure that permits future scalability as new discoveries continue to inexorably inform future editions

Exflagellated microgametes of <i> Plasmodium vivax</i> in human peripheral blood: A case report and review of the literature

Peripheral blood smear examination is the most specific as well as the most common test performed for the diagnosis of malaria. Schizonts, ring forms (trophozoites) and gametocytes are the stages of malarial parasite that are commonly seen in the peripheral blood smear of a patient. Here, we report an extremely rare case of a 40-year-old male patient who presented with <i> Plasmodium vivax</i> infection with multiple exflagellated microgametes in the peripheral blood smear with review of the literature. Exflagellation of microgametes in malarial parasites is only seen in the definitive host, mosquito, and is very unusual to see during the developmental phases in the intermediate host, human. It is important to recognize these exflagellated microgametes in the peripheral blood smear as they may lead to diagnostic confusion with organisms such as spirochetes and trypanosomes

PERFORMANCE EVALUATION OF VARIOUS IMAGE-PROCESSING TECHNIQUES FOR THE MEASUREMENT OF WATER SURFACE VELOCITY

Long-muffle estimation of water speed is a testing undertaking. Diverse endeavoursto gauge flight in a liquid from a smooth stream with physical contact sensors couldn't deliver precise results. Estimation of water speed with direct contact sensor which results mechanical torment and high upkeep cost. Theexpectationof this paper is to utensils the non-contact water stream speed estimation framework scilicet in light of a picture based strategy. This paper shows a methodology for assessing water stream speed by taking after the development of particles through a progression of pictures. The picture based water stream estimation recognizes the worktop elements and measures their positions regarding this present reality by using Particle Matching based on Centroid Tracking Method &amp; Block Matching Algorithm (BMA)

Performance Evaluation Of Various Image-processing Techniques For The Measurement Of Water Surface Velocity

Long-muffle estimation of water speed is a testing undertaking. Diverse endeavoursto gauge flight in a liquid from a smooth stream with physical contact sensors couldn't deliver precise results. Estimation of water speed with direct contact sensor which results mechanical torment and high upkeep cost. Theexpectationof this paper is to utensils the non-contact water stream speed estimation framework scilicet in light of a picture based strategy. This paper shows a methodology for assessing water stream speed by taking after the development of particles through a progression of pictures. The picture based water stream estimation recognizes the worktop elements and measures their positions regarding this present reality by using Particle Matching based on Centroid Tracking Method &amp; Block Matching Algorithm (BMA)

Intracytoplasmic antigen study by flow cytometry in hematolymphoid neoplasm

Flow cytometric detection of intracellular antigens has become a standard method in establishing proper leukemic cell lineage affiliation. It has a non-debatable contribution to the diagnosis of hematolymphoid neoplasm as well as in minimal residual disease. Combination of analysis of fluorescence labeling and light scatter properties of cells allows rapid and better determination of target cell antigens. Regarding the detection of intracellular antigens, standardization of the procedure remains, however, a real challenge. Detection of intracellular antigens by flow cytometry (FCM) requires effective fixation and permeabilization of the cell membrane. In the available literature, some reports describe methodologies to achieve satisfactory results for detection of either cytoplasmic or nuclear antigens; however, no methodological consensus has yet been achieved among the laboratories. This article is an attempt to describe different approaches to detect intracellular molecules by FCM

PERFORMANCE EVALUATION OF VARIOUS IMAGE PROCESSING TECHNIQUE FOR THE MEASUREMENT OF WATER SURFACE VELOCITY

Long-muffle estimation of water speed is a testing undertaking. Diverse endeavoursto gauge flight in a liquid from a smooth stream with physical contact sensors couldn't deliver precise results. Estimation of water speed with direct contact sensor which results mechanical torment and high upkeep cost. Theexpect of this paper is to utensils the non-contact water stream speed estimation framework scilicet in light of a picture based strategy. This paper shows a methodology for assessing water stream speed by taking after the development of particles through a progression of pictures. The picture based water stream estimation recognizes the worktop elements and measures their positions regarding this present reality by using Particle Matching based on Centroid Tracking Method &amp; Block Matching Algorithm (BMA)

Performance Evaluation of Various Image Processing Technique for the Measurement of Water Surface Velocity

Long-muffle estimation of water speed is a testing undertaking. Diverse endeavoursto gauge flight in a liquid from a smooth stream with physical contact sensors couldn't deliver precise results. Estimation of water speed with direct contact sensor which results mechanical torment and high upkeep cost. Theexpect of this paper is to utensils the non-contact water stream speed estimation framework scilicet in light of a picture based strategy. This paper shows a methodology for assessing water stream speed by taking after the development of particles through a progression of pictures. The picture based water stream estimation recognizes the worktop elements and measures their positions regarding this present reality by using Particle Matching based on Centroid Tracking Method &amp; Block Matching Algorithm (BMA)

Stability and Degradation Kinetic study of Bilastine in Solution State by RP-HPLC Method

Aim: The current study dealt with the degradation behavior of Bilastine and degradation kinetics of a drug in solution state. Background:&nbsp; Very limited information on the effect of pH on maximum stability has been published. In order to understand the degradation kinetics of bilastine, aqueous stability studies were carried out, because such studies on bilastine have not been reported in the literature, further no methods have reported about shelf-life determination of bilastine. The study design involves selection of stability indicating RP-HPLC method for estimation of drug then evaluation of degradation kinetics, shelf-life determination and validation of proposed method. Results: The Shimadzu HPLC series 1100 was used for stress degradation analysis of bilastine in tablet dosage form. The analysis was performed using Agilent ZORBAX SB-C8 (4.6×150×5µm) column and Phosphate Buffer: Acetonitrile (pH-5.0) in the ratio of 60:40 as mobile phase; wavelength selected for analysis was 254nm with the flow rate of 1mL/min at which drug showed sharp peak. The analysis was performed on the isocratic pump mode with the injection volume of 20µl. The mobile phase is used as diluent. The proposed method was found to be linear over the range 10 to 50 µg/mL. The analysis was performed by placing standard and samples with 7 different pH buffer, oxidative and neutral hydrolytic solutions in oven at 40ºC, 60⁰C and room temperature for an interval of 30, 60, 90, 120, 150, 180 mints for standard and samples. The results indicated that the pH, temperature, ionic strength and oxidation greatly influence the stability of Bilastine and the degradation behavior of Bilastine followed pseudo-first-order kinetics. Bilastine was most stable in neutral, alkaline, lower temperature conditions and lower ionic strength. Conclusion: The proposed method was found to be specific, selective and robust and successfully applied for its assay, degradation (stress testing) of drug and degradation kinetics in solution state. Keywords: Degradation, Stability, Bilastine, RP-HPLC, Kinetic

Biclonal chronic lymphocytic leukemia: A study of two cases and review of literature

Chronic lymphocytic leukemia (CLL) is a common, immunophenotypically well-defined mature B-cell neoplasm. Demonstration of more than 5000/μL CD5+ B-cell population with co-expression of CD23, weak expression of CD20, and one type of immunoglobin light chain (either kappa or lambda) is necessary for the diagnosis of CLL. However, CLL with two populations of B-cells expressing both kappa as well as lambda (biclonal) light chains are extremely rare and has not been reported from India. We report two cases of biclonal CLL presented with leukocytosis, typical morphological features, and distinct immunophenotype of CLL. These cases are also an example which suggests that careful attention to the morphology of the blood smear and the entire immunophenotype panel is a must and will aid the proper diagnosis as only light chain ratios can be misguiding