Psicoterapia breve de emergencia como apoyo a niños y niñas víctimas de maltrato infantil, que asisten a la procuraduría de los derechos humanos de la ciudad capital, Guatemala.

Aplicar Psicoterapia Breve de Emergencia, a niños y niñas. Se observó que se minimizaron síntomas o desadaptaciones que presentaban, lo cual ayudo a cada uno de los niños a reafirmar la autoestima, autoconcepto y autocontrol, en un corto tiempo. El presente trabajo de investigación se realizado con niños y niñas comprendidos entre la primera y segunda infancia, (1 a 12 años) cada niño y niña presentó diferente tipo de maltrato (físico, emocional, por abandono o negligencia y abuso sexual). La muestra fue no homogénea. Se realizó un estudio de casos, para tratar en forma individual las manifestaciones emocionales, conductuales y físicas. Se elaboró una historia clínica, la cual fue aplicada al inicio para obtener toda la información concisa y precisa de cada caso recibido. Seguidamente se aplicó una hoja de observación directa, la cual consistía en cinco tipos de trastornos (conductuales, alimentación, sueño, fisiológicos y de lenguaje). Se chequeo con la premisa que el niño o la niña manifestaba, y en base a ello se brindó la psicoterapia breve de emergencia. Se puede decir que la Psicoterapia Breve de Emergencia se utiliza muy pocas veces en nuestro ámbito por lo que en esta investigación narramos los beneficios de la misma en el estudio de casos de niños y niñas víctimas de maltrato infantil y de qué manera llegan a manejar sus emociones

Tourist product: my astronomical circuit

The present work which is a tourist product with the name of my astronomical circuit, is being worked on for the purpose of creating a product which must be innovated, competitive, environmentally friendly that exceed the expectations of tourists which can be nationals or internationals people. By reading this document many people will feel motivated and interested in investing time and money in tourism and they will see this area with another different perspective. As well as this work has been created with the intention of presenting a new touristic product which is called my astronomical circuit “. This touristic product can be described as a new place to visit if people enjoy watching the stars at night or just to escape from the city and have a breath in a small town away from it. This place is specifically located in San Juan Talpa; department of La Paz. Finally, we will show the product cost as well as the calculation to stablish a price per tour; which is the service we will provide to our customer, it is important in all type of entrepreneurship that we take in to account all the direct and indirect costs for the facilities to be in good standard; along with it, we will present the logo and the name which have been carefully studied to attract the interest of tourist as this makes a strong first impression and it is the foundation of a brand identity, must be memorable, separates you from the competition and fosters brand loyalty; at the end, this work will help us taking our first steps to create a new service that can become in a great one. Key words: tourism ; entrepreneur ; economy ; product ; innovation

Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensor

Biosensors based on graphene field-effect transistors have become a promising tool for detecting a broad range of analytes. However, their performance is substantially affected by the functionalization protocol. In this work, we use a controlled in-vacuum physical method for the covalent functionalization of graphene to construct ultrasensitive aptamer-based biosensors (aptasensors) able to detect hepatitis C virus core protein. These devices are highly specific and robust, achieving attomolar detection of the viral protein in human blood plasma. Such an improved sensitivity is rationalized by theoretical calculations showing that induced polarization at the graphene interface, caused by the proximity of covalently bound molecular probe, modulates the charge balance at the graphene/aptamer interface. This charge balance causes a net shift of the Dirac cone providing enhanced sensitivity for the attomolar detection of the target proteins. Such an unexpected effect paves the way for using this kind of graphene-based functionalized platforms for ultrasensitive and real-time diagnostics of different diseases.EU Graphene Flagship funding (Grant Graphene Core3 881603), the Ministerio de Ciencia e Innovación of Spain: PID2020-113142RB-C21, the European Structural Funds via FotoArt-CM project (P2018/NMT-4367) and the Portuguese Foundation for Science and Technology (FCT) via the Strategic Funding UIDB/04650/2020. Work at CAB was funded by the Spanish Ministerio de Ciencia e Innovación (MICINN) grant no. PID2019-104903RB-I00 and the Spanish Agencia Estatal de Investigación (AEI) Project no. MDM-2017-0737 - Unidad de Excelencia “María de Maeztu,” and it also benefits from the interdisciplinary framework provided by CSIC through “LifeHUB.CSIC” initiative (PIE 202120E047-CONEXIONES-LIFE). CIBERehd is funded by Instituto de Salud Carlos III (ISCIII). A.N. is supported by the predoctoral fellowship PRE-CAB-BIOMOLECULAS 2 from INTA. B.T-V. is supported by the predoctoral fellowship TS17/16 from INTA and by the CSIC “Garantía Juvenil” contract CAM19_PRE_CAB_001 funded by Comunidad de Madrid (CAM). FCT supports T.D. and P.C. under Ph.D. grants SFRH/BD/08181/2020 and SFRH/BD/128579/2017. M.M. would like to thank Comunidad de Madrid for the predoctoral grant IND2020/BIO-17523. P.A. and C.B. also acknowledge the support provided by La Caixa Foundation through Project LCF/PR/HR21/52410023. L. V. would like to thank Comunidad de Madrid (TRANSNANOAVANSENS program: S2018-NMT-4349) and E.V. García-Frutos for her assistance during the AFM experiments

Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensor

This study presents the development of a lab-on-a-chip (LoC) by integrating a graphene field-effect transistor (FET) chip with a programmable microfluidic device for DNA detection. The real-time biochemical events on the graphene FET chip were monitored through Dirac voltage shift data from the portable graphene curve reader with changes dependent on the fluidic flow into the sensing interface by a fully automated programmable microfluidic system. High sensitivity with high reliability can be obtained with a nine-graphene sensor layout on a single chip. The portable graphene curve reader also provides a tunable electrical parameter setup and straightforward data acquisition. Fluidic control was performed through a multi-position valve, allowing sequential commands for liquid injection into the polydimethylsiloxane (PDMS) flow cell mounted on the sensing chip. The flow cell design with impinging jet geometry and the microfluidic system packaging offer high precision and portability as a less laborious and low-cost sensing setup. The merged system allows for various functionalities, including probe DNA (pDNA) immobilization, a blocking step, and DNA hybridization with stable signal output autonomously, even in a long-run experimental setup. As a DNA sensor, the proposed prototype has demonstrated a high sensitivity of ~44 mV/decade of target DNA concentration, with an outstanding limit of detection (LoD) of ~0.642 aM, making it one of the most sensitive sensors reported up to date. The programmable device has demonstrated essential versatilities for biomolecular detection in a fully portable and automated platform.This research is supported by PORTGRAPHE-Control of Port and Douro Wines authenticity using graphene DNA sensors project co-funded by Fundação para a Ciência e a Tecnologia (FCT) Portugal (PTDC/BIA-MOL/31069/2017) and the ERDF through COMPETE2020 (POCI-01–0145-FEDER-031069). One of the authors (Telma Domingues) acknowledges a Ph.D. grant from Fundação para a Ciência e a Tecnologia (FCT) Portugal (SFRH/BD/08181/2020). FCT partially supported University of Minho´s research in the Strategic Funding UIDB/04650/2020

II Update Day in Speech Therapy: Interdisciplinary clinical approach to swallowing and voice

El acceso a la justicia y a las problemáticas que la rodean no deja de ser un hecho pertinente ante la realidad del país. Son múltiples las barreras existentes no solo para acceder al aparato judicial, sino también para garantizar el correcto ejercicio de los derechos humanos. Por lo anterior, la Universidad del Rosario, en cabeza del Observatorio Legislativo y del Grupo de Investigación en Derechos Humanos, en asociación con la Fundación Hanns Seidel, culminó con éxito la décima versión del Concurso Nacional de Semilleros de Investigación titulado “Diálogos interdisciplinarios sobre los problemas de la justicia en Colombia”, el cual, por primera vez en la historia, se realizó de manera virtual, dadas las circunstancias generadas por la pandemia COVID-19. El cierre del evento fue aún más contundente con la publicación de este libro, que contiene las ponencias de los estudiantes ganadores. En esta oportunidad, el objetivo del concurso fue brindar un espacio propicio de investigación, generación de conocimiento y fortalecimiento de habilidades para los estudiantes interesados en reflexionar en torno al acceso a la justicia y sus barreras desde diferentes frentes: estructura institucional; grupos de especial protección constitucional; eficacia, eficiencia y transparencia; métodos alternativos en resolución de conflictos; justicia diferencial; justicia en los territorios; jurisdicciones especiales y justicia digital.Access to justice and the problems that surround it is still a pertinent fact in the reality of the country. There are multiple barriers not only to access the judicial apparatus, but also to guarantee the correct exercise of human rights. Therefore, the Universidad del Rosario, at the head of the Legislative Observatory and the Human Rights Research Group, in association with the Hanns Seidel Foundation, successfully completed the tenth version of the National Competition of Research Seedlings entitled ""Interdisciplinary Dialogues on the justice problems in Colombia ”, which, for the first time in history, was carried out virtually, given the circumstances generated by the COVID-19 pandemic. The closing of the event was even more forceful with the publication of this book, which contains the presentations of the winning students. On this occasion, the objective of the contest was to provide a conducive space for research, generation of knowledge and strengthening of skills for students interested in reflecting on access to justice and its barriers from different fronts: institutional structure; groups of special constitutional protection; effectiveness, efficiency and transparency; alternative methods in conflict resolution; differential justice; justice in the territories; special jurisdictions and digital justice

Biocapteur de détection du virus de l'hépatite C

[EN] The present invention relates to an electrolyte-gated graphene field-effect transistor (G-FET) based biosensor characterized in that it comprises an aptamer against core protein of hepatitis C virus (HCV), particularly the aptamer comprising the nucleotide sequence SEQ ID NO:1, which allows the detection of core protein of HCV in the attomolar range both in buffer and in human plasma, greatly improving the sensitivity and specificity of the detection of HCV in biological samples.[FR] La présente invention concerne un biocapteur de transistor à effet de champ de graphène (G-FET) à grille électrolytique caractérisé en ce qu'il comprend un aptamère contre une protéine nucléaire du virus de l'hépatite C (VHC), en particulier l'aptamère comprenant la séquence nucléotidique SEQ ID NO : 1, ce qui permet la détection de la protéine nucléaire du VHC dans la plage attomolaire à la fois dans le tampon et dans le plasma humain, ce qui améliore considérablement la sensibilité et la spécificité de la détection du VHC dans des échantillons biologiques.Peer reviewedConsejo Superior de Investigaciones Científicas, Instituto Nacional de Técnica Aeroespacial, International Iberian Nanotechnology Laboratory, Universidade do MinhoA1 Solicitud de patente con informe sobre el estado de la técnic