Descripción del perfil proteomico del plasma seminal de asininos de la raza Pêga

A ausência da categoria asinina nas normas técnicas de avaliação seminal promove a necessidade de informações reprodutivas desta espécie. Objetivou-se descrever o perfil proteômico do plasma seminal de jumentos da raça Pêga (Equus asinus). Utilizaram-se seis animais púberes, com peso 239±32,6 Kg e circunferência escrotal de 36,8±6,9 cm, criados em mesma propriedade no estado do Tocantins, Brasil. Foi realizada eletroforese unidimensional, utilizando 12,5% de acrilamida e 30μg de proteína. As bandas foram descoradas e digeridas com tripsina para análise em espectrômetro de massa ESI-Q-TOF. Através de bioinformática, pelo banco de dados UniProtKB, as proteínas foram identificadas. Os termos da ontologia genética foram obtidos a partir do software STRAP®. A média da concentração proteica do plasma seminal foi 23,6±12,6 μg/μL. Foram detectadas pelo menos 26 bandas por animal (QuantityOne®). Um total de 19 bandas e 52 proteínas, com pesos entre 9,51 e 155,9 kDa, foram identificadas pela espectrometria. Os processos biológicos mais relevantes ligados às proteínas identificadas foram a regulação (24%) e processo celular (22%). As funções moleculares das proteínas foram descritas como ligação (42%) e atividade catalítica (31%). Em conclusão, a existência da descrição do padrão eletroforético destas proteínas plasmáticas seminais contribuirão com a construção de parâmetros para fertilidade.Palavras-chave: Proteômica, eletroforese, sêmen.The absence of the asinine category in the technical standards of seminal evaluation, promotes the need for reproductive information of this species. The objective of this study was to describe the proteomic profile of seminal plasma of Pêga donkeys (Equus asinus). Six pubertal animals, weighing 239±32.6 Kg and scrotal circumference of 36.8±6.9 cm, were raised in same farm in the state of Tocantins, Brazil. One-dimensional electrophoresis was performed using 12.5% acrylamide and 30μg protein. The bands were destained and digested with trypsin for analysis on ESI-Q-TOF mass spectrometer. Through bioinformatics, through the UniProtKB database, the proteins were identified. The terms of the genetic ontology were obtained from the software STRAP®. The mean protein concentration of seminal plasma was 23.6±12.6 μg/μL. At least 26 bands per animal were detected (QuantityOne®). A total of 19 bands and 52 proteins, with weights between 9.51 and 155.9 kDa, were identified by spectrometry. The most relevant biological processes related to the identified proteins were regulation (24%) and cellular process (22%). The molecular functions of the proteins were described as binding (42%) and catalytic activity (31%). In conclusion, the existence of a description of the electrophoretic pattern of these seminal plasma proteins will contribute to the construction of parameters for fertility.Keywords: Proteomics, electrophoresis, semen.La ausencia de la categoría asinina en las normas técnicas de evaluación seminal promueve la necesidad de información reproductiva da especie. Se objetivó describir el perfil proteómico del plasma seminal de asnos de la raza Pêga (Equus asinus). Se utilizaron seis animales púberes, con peso 239±32,6 Kg y circunferencia escrotal de 36,8±6,9 cm, creados en misma granja del estado de Tocantins, Brasil. Se realizó una electroforesis unidimensional, utilizando 12,5% de acrilamida y 30μg de proteína. Las bandas fueron blanqueadas y digeridas con tripsina para análisis en espectrómetro de masa ESI-Q-TOF. Através de bioinformática, por la base de datos UniProtKB, las proteínas fueron identificadas. Los términos de la ontología genética se obtuvieron a partir del software STRAP®. La media de la concentración proteica del plasma seminal fue de 23,6±12,6 μg/μL. Se detectaron al menos 26 bandas por animal (QuantityOne®). Un total de 19 bandas y 52 proteínas, con pesos entre 9,51 y 155,9 kDa, fueron identificadas por la espectrometría. Los procesos biológicos más relevantes relacionados con las proteínas fueron la regulación (24%) y el proceso celular (22%). Las funciones moleculares se describieron como enlace (42%) y actividad catalítica (31%). En conclusión, la existencia de la descripción del patrón electroforético de estas proteínas plasmáticas seminales contribuirán a la construcción de parámetros para la fertilidad.Descriptores: Proteómica, electroforesis, semen

Práticas fisioterapêuticas para o tratamento das disfunções temporomandibulares: avaliação do conhecimento de cirurgiões-dentistas

Introduction: Temporomandibular disorder (TMD) is one of the most prevalent orofacial conditions and because it is multifactorial, it requires multidisciplinary treatment. The number of people with TMD signs or symptoms is frequent, however, few are aware of this dysfunction and seek treatment. The lack of multidisciplinary communication or the lack of knowledge on the part of dentists in relation to physical therapists, often prevent multidisciplinary treatment. The aim of this research was to investigate the knowledge of dentists on the role of Physiotherapy in the treatment of TMD. Methodology: This is a quantitative, analytical cross-sectional study. 29 dental surgeons who worked in the private and / or public sector, aged between 25 and 60 years, of both genders, were investigated. Results: The research results showed that there is no efficient referral of patients with TMD by dentists to physiotherapists, so that a more effective treatment occurs. It was found that this situation did not occur due to the lack of knowledge of dentists (83.0%) about the role of Physiotherapy. Only 38% had already made this type of referral. Among the same 97% believe in the importance of multidisciplinary treatment. However, the interviewees emphasized their opinion on the importance of communication between the areas involved, however, they did not express the reason for the lack of referrals and integration among professionals. Among the participants, 48% stated that their first contact with this type of interdisciplinary information was during graduation. Conclusion: Few dental surgeons refer patients with TMD to physiotherapists, therefore it is necessary to disclose more results from the evolution of clinical cases in the area of Dentistry, in addition to conducting more focused research to concretize interdisciplinary thoughts and attitudes among professionals in the field. general health within the treatment of TMD.Introdução: A disfunção temporomandibular (DTM) é uma das condições orofaciais mais prevalentes e por ser multifatorial, necessita de tratamento multidisciplinar. É frequente o número de pessoas que apresentam sinais ou sintomas de DTM, porém, poucas são conhecedoras desta disfunção e buscam por tratamento. A falta de comunicação multidisciplinar ou a ausência de conhecimento por parte dos cirurgiões-dentistas em relação aos fisioterapeutas, muitas vezes impedem o tratamento multidisciplinar. O objetivo da presente pesquisa foi investigar o conhecimento dos cirurgiões-dentistas sobre a atuação da Fisioterapia no tratamento das DTM. Metodologia: Trata-se de um estudo quantitativo, do tipo analítico transversal. Foram investigados 29 cirurgiões-dentistas que trabalhavam no setor privado e/ou público, com idades entre 25 e 60 anos, de ambos os gêneros. Resultados: Os resultados da pesquisa mostraram que não existe um encaminhamento eficiente dos pacientes com DTM por parte dos cirurgiões-dentistas aos fisioterapeutas, para que assim ocorra um tratamento mais eficaz. Foi constatado que essa situação não ocorria por falta de conhecimento dos cirurgiões-dentistas (83,0%), sobre a atuação da Fisioterapia. Apenas 38% já tinham feito esse tipo de encaminhamento. Entre os mesmos 97% acreditam na importância do tratamento multidisciplinar. No entanto, os entrevistados ressaltaram a sua opinião sobre a importância da comunicação entre as áreas envolvidas, porém não manifestaram a razão da falta de encaminhamentos e da integração entre os profissionais. Entre os participantes 48% afirmaram ter sido na graduação o primeiro contato com esse tipo de informação interdisciplinar. Conclusão: Poucos cirurgiões-dentistas encaminham os pacientes com DTM para os fisioterapeutas, portanto faz-se necessário divulgar mais resultados das evoluções de casos clínicos para a área da Odontologia, além de realizar pesquisas mais focadas em concretizar pensamentos e atitudes interdisciplinares entre os profissionais da saúde de forma geral dentro do tratamento da DTM

Neotropical freshwater fisheries : A dataset of occurrence and abundance of freshwater fishes in the Neotropics

The Neotropical region hosts 4225 freshwater fish species, ranking first among the world's most diverse regions for freshwater fishes. Our NEOTROPICAL FRESHWATER FISHES data set is the first to produce a large-scale Neotropical freshwater fish inventory, covering the entire Neotropical region from Mexico and the Caribbean in the north to the southern limits in Argentina, Paraguay, Chile, and Uruguay. We compiled 185,787 distribution records, with unique georeferenced coordinates, for the 4225 species, represented by occurrence and abundance data. The number of species for the most numerous orders are as follows: Characiformes (1289), Siluriformes (1384), Cichliformes (354), Cyprinodontiformes (245), and Gymnotiformes (135). The most recorded species was the characid Astyanax fasciatus (4696 records). We registered 116,802 distribution records for native species, compared to 1802 distribution records for nonnative species. The main aim of the NEOTROPICAL FRESHWATER FISHES data set was to make these occurrence and abundance data accessible for international researchers to develop ecological and macroecological studies, from local to regional scales, with focal fish species, families, or orders. We anticipate that the NEOTROPICAL FRESHWATER FISHES data set will be valuable for studies on a wide range of ecological processes, such as trophic cascades, fishery pressure, the effects of habitat loss and fragmentation, and the impacts of species invasion and climate change. There are no copyright restrictions on the data, and please cite this data paper when using the data in publications

Brazilian Flora 2020: Leveraging the power of a collaborative scientific network

International audienceThe shortage of reliable primary taxonomic data limits the description of biological taxa and the understanding of biodiversity patterns and processes, complicating biogeographical, ecological, and evolutionary studies. This deficit creates a significant taxonomic impediment to biodiversity research and conservation planning. The taxonomic impediment and the biodiversity crisis are widely recognized, highlighting the urgent need for reliable taxonomic data. Over the past decade, numerous countries worldwide have devoted considerable effort to Target 1 of the Global Strategy for Plant Conservation (GSPC), which called for the preparation of a working list of all known plant species by 2010 and an online world Flora by 2020. Brazil is a megadiverse country, home to more of the world's known plant species than any other country. Despite that, Flora Brasiliensis, concluded in 1906, was the last comprehensive treatment of the Brazilian flora. The lack of accurate estimates of the number of species of algae, fungi, and plants occurring in Brazil contributes to the prevailing taxonomic impediment and delays progress towards the GSPC targets. Over the past 12 years, a legion of taxonomists motivated to meet Target 1 of the GSPC, worked together to gather and integrate knowledge on the algal, plant, and fungal diversity of Brazil. Overall, a team of about 980 taxonomists joined efforts in a highly collaborative project that used cybertaxonomy to prepare an updated Flora of Brazil, showing the power of scientific collaboration to reach ambitious goals. This paper presents an overview of the Brazilian Flora 2020 and provides taxonomic and spatial updates on the algae, fungi, and plants found in one of the world's most biodiverse countries. We further identify collection gaps and summarize future goals that extend beyond 2020. Our results show that Brazil is home to 46,975 native species of algae, fungi, and plants, of which 19,669 are endemic to the country. The data compiled to date suggests that the Atlantic Rainforest might be the most diverse Brazilian domain for all plant groups except gymnosperms, which are most diverse in the Amazon. However, scientific knowledge of Brazilian diversity is still unequally distributed, with the Atlantic Rainforest and the Cerrado being the most intensively sampled and studied biomes in the country. In times of “scientific reductionism”, with botanical and mycological sciences suffering pervasive depreciation in recent decades, the first online Flora of Brazil 2020 significantly enhanced the quality and quantity of taxonomic data available for algae, fungi, and plants from Brazil. This project also made all the information freely available online, providing a firm foundation for future research and for the management, conservation, and sustainable use of the Brazilian funga and flora