Description and molecular phylogeny of a new and one known needle nematode of the genus Paralongidorus (Nematoda: Longidoridae) from grapevine in Portugal

A new and a known longidorid nematode, Paralongidorus lusitanicus n. sp. and Paralongidorus plesioepimikis, are described and illustrated from populations extracted from soil associated with grapevine (Vitis vinifera L.) from Escaroupim and Pó (central-Western Portugal), respectively. The new needle nematode P. lusitanicus n. sp. is characterised by a very large body size (8072–12,022 μm), an expanded and rounded lip region, ca 30 μm wide, with a clear constriction followed by a depression posterior to the amphidial aperture, amphidial fovea very large (11.0–19.0 μm), stirrup-shaped, with conspicuous slit-like aperture as shown in scanning electron microscopy studies, a very long and flexible odontostyle (180.0–223.0 μm), guiding ring located at 28.0–41.5 μm from anterior end, vulva anterior to the mid-body (34–41%), a dorsally convex-conoid tail with rounded terminus (29–42 μm long), bearing two or three pairs of caudal pores and males common (ratio 1:1.6 females) with spicules ca 80 μm long. Morphological and morphometric traits for P. plesioepimikis fit well with the original description, and is reported for the first time in Portugal. Integrative diagnosis of both species was completed with molecular data obtained using D2-D3 expansion segments of 28S rDNA, ITS1-rDNA and partial 18S–rDNA. The phylogenetic relationships of these species with other Paralongidorus spp. using these three molecular markers indicated that P. lusitanicus n. sp. clustered together with other Paralongidorus spp. forming a sister clade with P. plesioepimikis, both of them sharing a large body, long odontostyle, an anteriorly located vulva and an expanded and rounded lip region with a clear constriction followed by a depression posterior to the amphidial aperture

Tameness of the pseudovariety LS1

The notion of k-tameness of a pseudovariety was introduced by Almeida and Steinberg and is a strong property which implies decidability of pseudovarieties. In this paper we prove that the pseudovariety LSl, of local semilattices, is k-tame.This work was supported, in part, by FCT through the Centro de Matemática da Universidade do Minho, and by the FCT and POCTI approved project POCTI/32817/MAT/2000 which is comparticipated by the European Community Fund FEDER

Development and validation of an HPLC method for the quantitation of 1,3-dihydroxy-2-methylxanthone in biodegradable nanoparticles

A rapid and simple high-performance liquid chromatographic method for the analysis of 1,3-dihydroxy-2-methylxanthone (DHMXAN) in biodegradable poly(D,L-lactide-co-glycolide) (PLGA) nanosphere and nanocapsule formulations is developed and validated. The method does not require any complex sample extraction procedure. Chromatographic separation is made with a reversed-phase C18 column, using methanol-water (90:10, v/v) containing 1% (v/v) acetic acid as a mobile phase at a flow rate of 1 mL/min. Identification is made by UV detection at 237 nm. The isocratic system operates at ambient temperature and requires 7.5 min of chromatographic time. The developed method is statistically validated according to ICH guidelines and USP 29 for its specificity, linearity, accuracy, and precision. The assay method proposed in this study is specific for DHMXAN in the presence of nanosphere and nanocapsule excipients. Diode-array analyses confirm the purity of DHMXAN peak in stress conditions (> 99.0%). The method is shown to be linear (r 0.999) over the concentration range of 0.25-3.0 g/mL. Recovery ranges from 99.0% to 102.7% (RSD: 1.49%) and from 98.3% to 101.6% (RSD: 1.07%) for nanospheres and nanocapsules, respectively. Repeatability (intraassay precision) and intermediate precision is acceptable with RSD values ranging from 0.6% to 1.9% and from 0.3% to 2.0%, respectively. The method is shown to be suitable for the evaluation of DHMXAN content entrapped in PLGA nanoparticles

Semigroup presentations for test local groups

In this paper we exhibit a type of semigroup presentations which determines a class of local groups. We show that the finite elements of this class generate the pseudovariety LG of all finite local groups and use them as test-semigroups to prove that LG and S, the pseudovariety of all finite semigroups, verify the same kappa-identities involving kappa-terms of rank at most 1, where kappa denotes the implicit signature consisting of the multiplication and the (omega-1)-power.Fundação para a Ciência e a Tecnologia (FCT), under the project PEst-C/MAT/UI0013/2011European Regional Development Fund, through the programme COMPET

Semidirect product with an order-computable pseudovariety and tameness

Dedicated to the memory of Walter Douglas Munn.The semidirect product of pseudovarieties of semigroups with an ordercomputable pseudovariety is investigated. The essential tool is the natural representation of the corresponding relatively free profinite semigroups and how it transforms implicit signatures. Several results concerning the behavior of the operation with respect to various kinds of tameness properties are obtained as applications.ESF programme “Automata: from Mathematics to Applications (AutoMathA)”.Project PTDC/MAT/65481/2006, which is partly funded by the European Community Fund FEDER.Fundação para a Ciência e a Tecnologia (FCT) through the Centro de Matemática da Universidade do Porto and Centro de Matemática da Universidade do Minh

A new approach to ultrasonic degassing to improve the mechanical properties of aluminum alloys

Ultrasonic degassing of liquid metals has been studied over the last years, but it has been limited to laboratorial scale experiments of low volumes of melt. In this work, the combined effect of acoustic cavitation with metal agitation induced by the mechanical vibration of the ultrasonic radiator itself was studied, using a specially designed low frequency mechanical vibrator coupled to the ultrasonic degassing unit. Liquid motion in water was characterized by high speed digital Photron-FastCam APX RS video camera and Laser Doppler Anemometry to select the most favorable US and mechanical vibrator frequencies to induce suitable water stirring. Selected parameters were used to degas 10 L of AlSi9Cu3(Fe) alloy. A suitable piezoelectric sensor was used to measure sound pressure at different distances from the sonotrode to identify the zone of higher acoustic activity. Results have shown that melt stirring significantly improves US degassing efficiency (since it is possible to achieve almost the aluminum alloy theoretical density after 3 min processing time) which contributed to increase the tensile properties of the alloy.This research was supported by FEDER/COMPETE funds and by national funds through FCT - Portuguese Foundation for Science and Technology and was developed on the aim of the research Project PTDC/EME-TME/119658/2010 and the Post-Doctoral Grant SFRH/BPD/76680/2011. Acknowledgements also to the University of Minho, for the provision of research facilities.info:eu-repo/semantics/publishedVersio

Project-based learning in a mechanical engineering course: A new proposal based on student's views

The evolution of learning in higher education is nowadays evident. Several discussions and studies have been performed about new methodologies that can disrupt the way the classes are taught in universities. In this context, Project-Based Learning (PBL) is the most emphasized. In the Mechanical Engineering course at the University of Minho (UM), the Integration Project (IP) courses apply a PBL methodology, being these classes the differentiating element of the Integrated Master in Mechanical Engineering (IMME) compared with other Portuguese universities. However, even if the innovative aspect of this approach is recognized nationally, the opinions between students and Professors, about the structure and organization of this class, are still divided. In that sense, this work presents a new proposal for the IP courses in which the opinion of students and successful models implemented in international universities are considered. This study analyses the best PBL methodologies implemented in Engineering courses and presents a PBL model actually implemented at the IMME. This information is combined with the student's views obtained from a survey conducted at the Department of Mechanical Engineering (DEM), regarding the actual PBL model. Through this study, a new proposal for the IP courses is presented. This proposal intends to provide an effective answer to the necessity of the students, using successful tools and methodologies to improve the teaching and learning process in the IMME course. Through this proposal, it is expected to increase the learning process and motivation of the students making them better prepared for a productive profession.The first author would like to express her gratitude for the support given by the Portuguese Foundation for Science and Technology (FCT) and the MIT Portugal Program. This work has been supported by FCT - Fundacao para a Ciencia e Tecnologia within the R&D Units Project Scope: UIDB/00319/2020 (ALGORITMI Center) and R&D Units Project Scope UIDP/04077/2020 (METRICS Center)