17 research outputs found
Mechanistic study and potential therapeutic targets of synucleinopathies
Mon projet de thĂšse sâinscrit dans lâĂ©tude des synuclĂ©inopathies, une famille de maladies neurodĂ©gĂ©nĂ©ratives. Les trois principales synuclĂ©inopathies sont la maladie de Parkinson, lâatrophie multisystĂ©matisĂ©e et la dĂ©mence Ă corps de Lewy. Ces maladies sont caractĂ©risĂ©es par une perte de neurones dans des rĂ©gions cĂ©rĂ©brales spĂ©cifiques et la prĂ©sence dâinclusions intra-cytoplasmiques positives pour lâα-synuclĂ©ine dans les neurones (Corps de Lewy) ou dans les oligodendrocytes (Inclusions gliales cytoplasmiques). Les causes dâinduction de ces maladies restent encore inconnues et les traitements curatifs sont inexistants. Lâobjectif de mon travail de thĂšse visait Ă Ă©tudier les mĂ©canismes neurodĂ©gĂ©nĂ©ratifs et de potentielles cibles thĂ©rapeutiques dans le contexte des synuclĂ©inopathies. Je me suis tout dâabord intĂ©ressĂ©e aux mĂ©canismes impliquĂ©s dans la transmission de lâα-synuclĂ©ine issue de patients atteints de lâatrophie multisystĂ©matisĂ©e. Ce travail nous a permis de dĂ©velopper un potentiel nouveau modĂšle de lâatrophie multisystĂ©matisĂ©e chez la souris et le primate non-humain, par la transmission de lâα-synuclĂ©ine dans le cerveau. Dans un deuxiĂšme temps, nous nous sommes intĂ©ressĂ©s Ă des cibles thĂ©rapeutiques Ă©ventuelles pour la maladie de Parkinson dans un mĂȘme modĂšle animal de la pathologie. Nous avons pu vĂ©rifier lâefficacitĂ© et la pertinence de trois diffĂ©rentes stratĂ©gies ciblant plusieurs mĂ©canismes affectĂ©s dans la maladie de Parkinson dans le but dâinduire une protection des neurones dopaminergiques de la substance noire des souris. Nous avons pu dĂ©montrer une dĂ©rĂ©gulation des niveaux de zinc au cours de la pathologie qui a suscitĂ© lâintĂ©rĂȘt de cibler son homĂ©ostasie dans le cerveau Ă travers une molĂ©cule chĂ©latrice du zinc. Ensuite, la surexpression dâun facteur de transcription impliquĂ© dans la survie des neurones dopaminergiques ainsi que dans le stress oxydatif et le protĂ©asome a montrĂ© son intĂ©rĂȘt comme cible thĂ©rapeutique de la maladie de Parkinson. Enfin, une molĂ©cule anti-agrĂ©gative a aussi dĂ©montrĂ© sa capacitĂ© Ă induire une neuroprotection. En rĂ©sumĂ©, ces travaux montrent dâabord lâimportance de lâα-synuclĂ©ine dans la mise en place et la progression des synuclĂ©inopathies, mais aussi la nĂ©cessitĂ© de cibler dâautres mĂ©canismes dĂ©rĂ©gulĂ©s dans ces pathologies pour proposer des nouvelles stratĂ©gies thĂ©rapeutiques.My thesis focused on the study of synucleinopathies, a family of neurodegenerative diseases. The three main synucleinopathies are Parkinsonâs disease, multiple system atrophy, and dementia with Lewy bodies. These diseases are characterized by the loss of neurons in various brain regions and the presence of intracytoplasmic α-synuclein-positive inclusions. These inclusions are located either in neurons (Lewy bodies) or in oligodendrocytes (Glial cytoplasmic inclusions). The trigger and cause for the formation of these inclusions remain unknown, and no curative treatments currently exist. The objective of my thesis was to study the neurodegenerative mechanisms and potential therapeutic strategies of these synucleinopathies. For this, I was first interested in the mechanisms implicated in the transmission of α-synuclein from multiple system atrophy patients. This allowed us to develop a potential new model to study multiple system atrophy in both mice and non-human primates by spreading of α-synuclein within the brain. In the second part, we wanted to investigate potential therapeutic targets in the same model of Parkinsonâs disease. This study confirmed the efficacy and pertinence of three different strategies that target various mechanisms of Parkinsonâs disease to induce the protection of dopaminergic neurons of the substantia nigra in a mouse model. By modulating zinc levels, we demonstrated the importance of zinc concentrations in the brain and the therapeutic interest in targeting metal homeostasis via specific chelators. We then used viral vectors to overexpress a transcription factor implicated in dopaminergic neuron survival, oxidative stress and proteasome activity in the substantia nigra of a mouse model of Parkinsonâs disease. Finally, we used an anti-aggregative molecule to determine its efficacy in protecting neurons in the same mouse model. Altogether, this thesis work showed the implication of α-synuclein in triggering and propagating synucleinopathies, the importance of targeting this protein, and other dysregulated cellular mechanisms to discover potential therapies
A New Rise of Non-Human Primate Models of Synucleinopathies
Synucleinopathies are neurodegenerative diseases characterized by the presence of α-synuclein-positive intracytoplasmic inclusions in the central nervous system. Multiple experimental models have been extensively used to understand better the mechanisms involved in the pathogenesis of synucleinopathy. Non-human primate (NHP) models are of interest in neurodegenerative diseases as they constitute the highest relevant preclinical model in translational research. They also contribute to bringing new insights into synucleinopathyâs pathogenicity and help in the quest and validation of therapeutical strategies. Here, we reviewed the different NHP models that have recapitulated key characteristics of synucleinopathy, and we aimed to highlight the contribution of NHP in mechanistic and translational approaches for synucleinopathies
Etude des mécanismes et cibles thérapeutiques des synucléinopathies
My thesis focused on the study of synucleinopathies, a family of neurodegenerative diseases. The three main synucleinopathies are Parkinsonâs disease, multiple system atrophy, and dementia with Lewy bodies. These diseases are characterized by the loss of neurons in various brain regions and the presence of intracytoplasmic α-synuclein-positive inclusions. These inclusions are located either in neurons (Lewy bodies) or in oligodendrocytes (Glial cytoplasmic inclusions). The trigger and cause for the formation of these inclusions remain unknown, and no curative treatments currently exist. The objective of my thesis was to study the neurodegenerative mechanisms and potential therapeutic strategies of these synucleinopathies. For this, I was first interested in the mechanisms implicated in the transmission of α-synuclein from multiple system atrophy patients. This allowed us to develop a potential new model to study multiple system atrophy in both mice and non-human primates by spreading of α-synuclein within the brain. In the second part, we wanted to investigate potential therapeutic targets in the same model of Parkinsonâs disease. This study confirmed the efficacy and pertinence of three different strategies that target various mechanisms of Parkinsonâs disease to induce the protection of dopaminergic neurons of the substantia nigra in a mouse model. By modulating zinc levels, we demonstrated the importance of zinc concentrations in the brain and the therapeutic interest in targeting metal homeostasis via specific chelators. We then used viral vectors to overexpress a transcription factor implicated in dopaminergic neuron survival, oxidative stress and proteasome activity in the substantia nigra of a mouse model of Parkinsonâs disease. Finally, we used an anti-aggregative molecule to determine its efficacy in protecting neurons in the same mouse model. Altogether, this thesis work showed the implication of α-synuclein in triggering and propagating synucleinopathies, the importance of targeting this protein, and other dysregulated cellular mechanisms to discover potential therapies.Mon projet de thĂšse sâinscrit dans lâĂ©tude des synuclĂ©inopathies, une famille de maladies neurodĂ©gĂ©nĂ©ratives. Les trois principales synuclĂ©inopathies sont la maladie de Parkinson, lâatrophie multisystĂ©matisĂ©e et la dĂ©mence Ă corps de Lewy. Ces maladies sont caractĂ©risĂ©es par une perte de neurones dans des rĂ©gions cĂ©rĂ©brales spĂ©cifiques et la prĂ©sence dâinclusions intra-cytoplasmiques positives pour lâα-synuclĂ©ine dans les neurones (Corps de Lewy) ou dans les oligodendrocytes (Inclusions gliales cytoplasmiques). Les causes dâinduction de ces maladies restent encore inconnues et les traitements curatifs sont inexistants. Lâobjectif de mon travail de thĂšse visait Ă Ă©tudier les mĂ©canismes neurodĂ©gĂ©nĂ©ratifs et de potentielles cibles thĂ©rapeutiques dans le contexte des synuclĂ©inopathies. Je me suis tout dâabord intĂ©ressĂ©e aux mĂ©canismes impliquĂ©s dans la transmission de lâα-synuclĂ©ine issue de patients atteints de lâatrophie multisystĂ©matisĂ©e. Ce travail nous a permis de dĂ©velopper un potentiel nouveau modĂšle de lâatrophie multisystĂ©matisĂ©e chez la souris et le primate non-humain, par la transmission de lâα-synuclĂ©ine dans le cerveau. Dans un deuxiĂšme temps, nous nous sommes intĂ©ressĂ©s Ă des cibles thĂ©rapeutiques Ă©ventuelles pour la maladie de Parkinson dans un mĂȘme modĂšle animal de la pathologie. Nous avons pu vĂ©rifier lâefficacitĂ© et la pertinence de trois diffĂ©rentes stratĂ©gies ciblant plusieurs mĂ©canismes affectĂ©s dans la maladie de Parkinson dans le but dâinduire une protection des neurones dopaminergiques de la substance noire des souris. Nous avons pu dĂ©montrer une dĂ©rĂ©gulation des niveaux de zinc au cours de la pathologie qui a suscitĂ© lâintĂ©rĂȘt de cibler son homĂ©ostasie dans le cerveau Ă travers une molĂ©cule chĂ©latrice du zinc. Ensuite, la surexpression dâun facteur de transcription impliquĂ© dans la survie des neurones dopaminergiques ainsi que dans le stress oxydatif et le protĂ©asome a montrĂ© son intĂ©rĂȘt comme cible thĂ©rapeutique de la maladie de Parkinson. Enfin, une molĂ©cule anti-agrĂ©gative a aussi dĂ©montrĂ© sa capacitĂ© Ă induire une neuroprotection. En rĂ©sumĂ©, ces travaux montrent dâabord lâimportance de lâα-synuclĂ©ine dans la mise en place et la progression des synuclĂ©inopathies, mais aussi la nĂ©cessitĂ© de cibler dâautres mĂ©canismes dĂ©rĂ©gulĂ©s dans ces pathologies pour proposer des nouvelles stratĂ©gies thĂ©rapeutiques
Mechanistic study and potential therapeutic targets of synucleinopathies
Mon projet de thĂšse sâinscrit dans lâĂ©tude des synuclĂ©inopathies, une famille de maladies neurodĂ©gĂ©nĂ©ratives. Les trois principales synuclĂ©inopathies sont la maladie de Parkinson, lâatrophie multisystĂ©matisĂ©e et la dĂ©mence Ă corps de Lewy. Ces maladies sont caractĂ©risĂ©es par une perte de neurones dans des rĂ©gions cĂ©rĂ©brales spĂ©cifiques et la prĂ©sence dâinclusions intra-cytoplasmiques positives pour lâα-synuclĂ©ine dans les neurones (Corps de Lewy) ou dans les oligodendrocytes (Inclusions gliales cytoplasmiques). Les causes dâinduction de ces maladies restent encore inconnues et les traitements curatifs sont inexistants. Lâobjectif de mon travail de thĂšse visait Ă Ă©tudier les mĂ©canismes neurodĂ©gĂ©nĂ©ratifs et de potentielles cibles thĂ©rapeutiques dans le contexte des synuclĂ©inopathies. Je me suis tout dâabord intĂ©ressĂ©e aux mĂ©canismes impliquĂ©s dans la transmission de lâα-synuclĂ©ine issue de patients atteints de lâatrophie multisystĂ©matisĂ©e. Ce travail nous a permis de dĂ©velopper un potentiel nouveau modĂšle de lâatrophie multisystĂ©matisĂ©e chez la souris et le primate non-humain, par la transmission de lâα-synuclĂ©ine dans le cerveau. Dans un deuxiĂšme temps, nous nous sommes intĂ©ressĂ©s Ă des cibles thĂ©rapeutiques Ă©ventuelles pour la maladie de Parkinson dans un mĂȘme modĂšle animal de la pathologie. Nous avons pu vĂ©rifier lâefficacitĂ© et la pertinence de trois diffĂ©rentes stratĂ©gies ciblant plusieurs mĂ©canismes affectĂ©s dans la maladie de Parkinson dans le but dâinduire une protection des neurones dopaminergiques de la substance noire des souris. Nous avons pu dĂ©montrer une dĂ©rĂ©gulation des niveaux de zinc au cours de la pathologie qui a suscitĂ© lâintĂ©rĂȘt de cibler son homĂ©ostasie dans le cerveau Ă travers une molĂ©cule chĂ©latrice du zinc. Ensuite, la surexpression dâun facteur de transcription impliquĂ© dans la survie des neurones dopaminergiques ainsi que dans le stress oxydatif et le protĂ©asome a montrĂ© son intĂ©rĂȘt comme cible thĂ©rapeutique de la maladie de Parkinson. Enfin, une molĂ©cule anti-agrĂ©gative a aussi dĂ©montrĂ© sa capacitĂ© Ă induire une neuroprotection. En rĂ©sumĂ©, ces travaux montrent dâabord lâimportance de lâα-synuclĂ©ine dans la mise en place et la progression des synuclĂ©inopathies, mais aussi la nĂ©cessitĂ© de cibler dâautres mĂ©canismes dĂ©rĂ©gulĂ©s dans ces pathologies pour proposer des nouvelles stratĂ©gies thĂ©rapeutiques.My thesis focused on the study of synucleinopathies, a family of neurodegenerative diseases. The three main synucleinopathies are Parkinsonâs disease, multiple system atrophy, and dementia with Lewy bodies. These diseases are characterized by the loss of neurons in various brain regions and the presence of intracytoplasmic α-synuclein-positive inclusions. These inclusions are located either in neurons (Lewy bodies) or in oligodendrocytes (Glial cytoplasmic inclusions). The trigger and cause for the formation of these inclusions remain unknown, and no curative treatments currently exist. The objective of my thesis was to study the neurodegenerative mechanisms and potential therapeutic strategies of these synucleinopathies. For this, I was first interested in the mechanisms implicated in the transmission of α-synuclein from multiple system atrophy patients. This allowed us to develop a potential new model to study multiple system atrophy in both mice and non-human primates by spreading of α-synuclein within the brain. In the second part, we wanted to investigate potential therapeutic targets in the same model of Parkinsonâs disease. This study confirmed the efficacy and pertinence of three different strategies that target various mechanisms of Parkinsonâs disease to induce the protection of dopaminergic neurons of the substantia nigra in a mouse model. By modulating zinc levels, we demonstrated the importance of zinc concentrations in the brain and the therapeutic interest in targeting metal homeostasis via specific chelators. We then used viral vectors to overexpress a transcription factor implicated in dopaminergic neuron survival, oxidative stress and proteasome activity in the substantia nigra of a mouse model of Parkinsonâs disease. Finally, we used an anti-aggregative molecule to determine its efficacy in protecting neurons in the same mouse model. Altogether, this thesis work showed the implication of α-synuclein in triggering and propagating synucleinopathies, the importance of targeting this protein, and other dysregulated cellular mechanisms to discover potential therapies
A New Rise of Non-Human Primate Models of Synucleinopathies
International audienceSynucleinopathies are neurodegenerative diseases characterized by the presence of α-synuclein-positive intracytoplasmic inclusions in the central nervous system. Multiple experimental models have been extensively used to understand better the mechanisms involved in the pathogenesis of synucleinopathy. Non-human primate (NHP) models are of interest in neurodegenerative diseases as they constitute the highest relevant preclinical model in translational research. They also contribute to bringing new insights into synucleinopathyâs pathogenicity and help in the quest and validation of therapeutical strategies. Here, we reviewed the different NHP models that have recapitulated key characteristics of synucleinopathy, and we aimed to highlight the contribution of NHP in mechanistic and translational approaches for synucleinopathies
Autophagy in Synucleinopathy: The Overwhelmed and Defective Machinery
International audienceAlpha-synuclein positive-intracytoplasmic inclusions are the common denominators of the synucleinopathies present as Lewy bodies in Parkinson's disease, dementia with Lewy bodies, or glial cytoplasmic inclusions in multiple system atrophy. These neurodegenerative diseases also exhibit cellular dyshomeostasis, such as autophagy impairment. Several decades of research have questioned the potential link between the autophagy machinery and alpha-synuclein protein toxicity in synucleinopathy and neurodegenerative processes. Here, we aimed to discuss the active participation of autophagy impairment in alpha-synuclein accumulation and propagation, as well as alpha-synuclein-independent neurodegenerative processes in the field of synucleinopathy. Therapeutic approaches targeting the restoration of autophagy have started to emerge as relevant strategies to reverse pathological features in synucleinopathies
Targeting α-synuclein for PD Therapeutics: A Pursuit on All Fronts
International audienceParkinson's Disease (PD) is characterized both by the loss of dopaminergic neurons in the substantia nigra and the presence of cytoplasmic inclusions called Lewy Bodies. These Lewy Bodies contain the aggregated α-synuclein (α-syn) protein, which has been shown to be able to propagate from cell to cell and throughout different regions in the brain. Due to its central role in the pathology and the lack of a curative treatment for PD, an increasing number of studies have aimed at targeting this protein for therapeutics. Here, we reviewed and discussed the many different approaches that have been studied to inhibit α-syn accumulation via direct and indirect targeting. These analyses have led to the generation of multiple clinical trials that are either completed or currently active. These clinical trials and the current preclinical studies must still face obstacles ahead, but give hope of finding a therapy for PD with time
The Zinc Ionophore Clioquinol Reduces Parkinsonâs Disease Patient-Derived Brain Extracts-Induced Neurodegeneration
International audienceParkinson's disease (PD) is pathologically characterized by intracellular α-synuclein-rich protein aggregates, named Lewy bodies (LB), and by the progressive loss of dopaminergic neurons in the substantia nigra. Several heavy metals, including zinc (Zn), have been suggested to play a role in PD progression, although the exact role of Zn in neurodegeneration remains to be fully elucidated. To address this gap, we investigated the effects of Zn modulation on the progression of degeneration in mice injected with PD patient-derived LB-extracts carrying toxic α-synuclein aggregates. Zn modulation was achieved using either a clioquinol-enriched diet, a Zn ionophore that redistributes cellular Zn, or a Zn-enriched diet that increases Zn levels. Clioquinol treatment significantly prevented dopaminergic neurodegeneration and reduced α-synuclein-associated pathology in LB-injected mice, while no differences were observed with Zn supplementation. Biochemical analyses further demonstrate that the expression levels of vesicle-specific Zn transporter ZnT3 in the striatum of LB-injected mice treated with clioquinol were decreased, suggesting an intracellular redistribution of Zn. Additionally, we found that clioquinol modulates the autophagy-lysosomal pathway by enhancing lysosomal redistribution within the neuronal compartments. Collectively, we found that in vivo pharmacological chelation of Zn, by dampening Zn-mediated cytotoxicity, can result in an overall attenuation of PD-linked lysosomal alterations and dopaminergic neurodegeneration. The results support zinc chelation as a disease-modifying strategy for treating PD
LâDOPA regulates αâsynuclein accumulation in experimental parkinsonism
International audienceAimsWidespread accumulation of misfolded αâsynuclein aggregates is a key feature of Parkinsonâs disease (PD). Although the pattern and extent of αâsynuclein accumulation through PD brains is known, the impact of chronic dopamineâreplacement therapy (the goldâstandard pharmacological treatment of PD) on the fate of αâsynuclein is still unknown. Here, we investigated the distribution and accumulation of αâsynuclein in the 1âmethylâ4âphenylâ1,2,3,6âtetrahydropyridine (MPTP) nonâhuman primate of PD and determined the effect of chronic LâDOPA treatment on MPTPâinduced αâsynuclein pathology.MethodsWe measured the density of αâsynuclein and tau immunoâpositive neurons in the substantia nigra, putamen, hippocampal CA1 region, temporal cortex and dentate nucleus of control, MPTP and MPTP+LâDOPAâtreated monkeys. Moreover, we also extracted and quantified TritonâX (TX) soluble and insoluble αâsynuclein in putamen and hippocampus samples from a separate cohort of control, MPTP and MPTP+LâDOPAâtreated monkeys.ResultsMPTPâinduced αâsynuclein accumulation in NHP model of PD was not limited to the substantia nigra but also occurred in the putamen, hippocampal CA1 region and temporal cortex. Tau was increased only in the temporal cortex. Moreover, increased intraneuronal TX insoluble αâsynuclein was truncated, but not in the structural form of Lewy bodies. The MPTPâinduced increase in αâsynuclein levels was abolished in animals having received LâDOPA in all the brain regions, except in the substantia nigra.ConclusionsDopamine replacement therapy can dramatically ameliorate αâsynuclein pathology in the MPTP NHP model of PD. Therefore, patientâs dopaminergic medication should be systematically considered when assessing αâsynuclein as a biomarker for diagnosis, monitoring disease progression and response to diseaseâmodifying treatments
p53-dependent programmed necrosis controls germ cell homeostasis during spermatogenesis
International audienceThe importance of regulated necrosis in pathologies such as cerebral stroke and myocardial infarction is now fully recognized. However, the physiological relevance of regulated necrosis remains unclear. Here, we report a conserved role for p53 in regulating necrosis in Drosophila and mammalian spermatogenesis. We found that Drosophila p53 is required for the programmed necrosis that occurs spontaneously in mitotic germ cells during spermatogenesis. This form of necrosis involved an atypical function of the initiator caspase Dronc/Caspase 9, independent of its catalytic activity. Prevention of p53-dependent necrosis resulted in testicular hyperplasia, which was reversed by restoring necrosis in spermatogonia. In mouse testes, p53 was required for heat-induced germ cell necrosis, indicating that regulation of necrosis is a primordial function of p53 conserved from invertebrates to vertebrates. Drosophila and mouse spermatogenesis will thus be useful models to identify inducers of necrosis to treat cancers that are refractory to apoptosis