111 research outputs found

    Satellite soil moisture observations: applications in the UK and India

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    The India-UK Water Centre (IUKWC) promotes cooperation and collaboration between the complementary priorities of NERC-MoES water security research. The State of Science Brief was produced as an output from an India-UK Water Centre supported Pump Priming Project on Synergistic utilisation of EO-based soil moisture observations: Applications in the UK and India

    The approach to vortex reconnection

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    We present numerical solutions of the Gross--Pitaevskii equation corresponding to reconnecting vortex lines. We determine the separation of vortices as a function of time during the approach to reconnection, and study the formation of pyramidal vortex structures. Results are compared with analytical work and numerical studies based on the vortex filament method.Comment: 11 pages, 9 figure

    How accurate are parental responses concerning their fourth-grade children's school-meal participation, and what is the relationship between children's body mass index and school-meal participation based on parental responses?

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    <p>Abstract</p> <p>Background</p> <p>This article investigated (1) parental response accuracy of fourth-grade children's school-meal participation and whether accuracy differed by children's body mass index (BMI), sex, and race, and (2) the relationship between BMI and school-meal participation (based on parental responses).</p> <p>Methods</p> <p>Data were from four cross-sectional studies conducted from fall 1999 to spring 2003 with fourth-grade children from 13 schools total. Consent forms asked parents to report children's usual school-meal participation. As two studies' consent forms did not ask about lunch participation, complete data were available for breakfast on 1,496 children (51% Black; 49% boys) and for lunch on 785 children (46% Black; 48% boys). Researchers compiled nametag records (during meal observations) of meal participation on randomly selected days during children's fourth-grade school year for breakfast (average nametag days across studies: 7-35) and for lunch (average nametag days across studies: 4-10) and categorized participation as "usually" (≥ 50% of days) or "not usually" (< 50% of days). Weight and height were measured. Concerning parental response accuracy, marginal regression was used with agreement between parental responses and nametag records as the dependent variable; independent variables were BMI, age, sex, race, and study. Concerning a relationship between BMI and school-meal participation, marginal regression was used with BMI as the dependent variable; independent variables were breakfast participation, lunch participation, age, sex, race, and study.</p> <p>Results</p> <p>Concerning breakfast participation and lunch participation, 74% and 92% of parents provided accurate responses, respectively. Parental response accuracy was better for older children for breakfast and lunch participation, and for Black than White children for lunch participation. Usual school-meal participation was significantly related to children's BMI but in opposite directions -- positively for breakfast and inversely for lunch.</p> <p>Conclusions</p> <p>Parental response accuracy of children's school-meal participation was moderately high; however, disparate effects for children's age and race warrant caution when relying on parental responses. The BMI results, which showed a relationship between school-meal participation (based on parental responses) and childhood obesity, conflict with results from a recent article that used data from the same four studies and found no significant relationship when participation was based on nametag records compiled for meal observations.</p

    The Initial Step in Human Immunodeficiency Virus Type 1 GagProPol Processing Can Be Regulated by Reversible Oxidation

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    BACKGROUND: Maturation of human immunodeficiency virus type 1 (HIV-1) occurs upon activation of HIV-1 protease embedded within GagProPol precursors and cleavage of Gag and GagProPol polyproteins. Although reversible oxidation can regulate mature protease activity as well as retrovirus maturation, it is possible that the effects of oxidation on viral maturation are mediated in whole, or part, through effects on the initial intramolecular cleavage event of GagProPol. In order assess the effect of reversible oxidation on this event, we developed a system to isolate the first step in protease activation involving GagProPol. METHODOLOGY/PRINCIPAL FINDINGS: To determine if oxidation influences this step, we created a GagProPol plasmid construct (pGPfs-1C) that encoded mutations at all cleavage sites except p2/NC, the initial cleavage site in GagProPol. pGPfs-1C was used in an in vitro translation assay to observe the behavior of this initial step without interference from subsequent processing events. Diamide, a sulfhydral oxidizing agent, inhibited processing at p2/NC by >60% for pGPfs-1C and was readily reversed with the reductant, dithiothreitol. The ability to regulate processing by reversible oxidation was lost when the cysteines of the embedded protease were mutated to alanine. Unlike mature protease, which requires only oxidation of cys95 for inhibition, both cysteines of the embedded protease contributed to this inhibition. CONCLUSIONS/SIGNIFICANCE: We developed a system that can be used to study the first step in the cascade of HIV-1 GagProPol processing and show that reversible oxidation of cysteines of HIV-1 protease embedded in GagProPol can block this initial GagProPol autoprocessing. This type of regulation may be broadly applied to the majority of retroviruses

    Low-cost electronic sensors for environmental research: pitfalls and opportunities

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    Repeat observations underpin our understanding of environmental processes, but financial constraints often limit scientists’ ability to deploy dense networks of conventional commercial instrumentation. Rapid growth in the Internet-Of-Things (IoT) and the maker movement is paving the way for low-cost electronic sensors to transform global environmental monitoring. Accessible and inexpensive sensor construction is also fostering exciting opportunities for citizen science and participatory research. Drawing on 6 years of developmental work with Arduino-based open-source hardware and software, extensive laboratory and field testing, and incor- poration of such technology into active research programmes, we outline a series of successes, failures and lessons learned in designing and deploying environmental sensors. Six case studies are presented: a water table depth probe, air and water quality sensors, multi-parameter weather stations, a time-sequencing lake sediment trap, and a sonic anemometer for monitoring sand transport. Schematics, code and purchasing guidance to reproduce our sensors are described in the paper, with detailed build instructions hosted on our King’s College London Geography Environmental Sensors Github repository and the FreeStation project website. We show in each case study that manual design and construction can produce research-grade scientific instrumentation (mean bias error for calibrated sensors –0.04 to 23%) for a fraction of the conventional cost, provided rigorous, sensor-specific calibration and field testing is conducted. In sharing our collective experiences with build-it- yourself environmental monitoring, we intend for this paper to act as a catalyst for physical geographers and the wider environmental science community to begin incorporating low-cost sensor development into their research activities. The capacity to deploy denser sensor networks should ultimately lead to superior envi- ronmental monitoring at the local to global scales

    The sensitivity of the vortex filament method to different reconnection models

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    We present a detailed analysis on the effect of using different algorithms to model the reconnection of vortices in quantum turbulence, using the thin-filament approach. We examine differences between four main algorithms for the case of turbulence driven by a counterflow. In calculating the velocity field we use both the local induction approximation (LIA) and the full Biot-Savart integral. We show that results of Biot-Savart simulations are not sensitive to the particular reconnection method used, but LIA results are.Comment: 9 pages, 9 figure

    Tree method for quantum vortex dynamics

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    We present a numerical method to compute the evolution of vortex filaments in superfluid helium. The method is based on a tree algorithm which considerably speeds up the calculation of Biot-Savart integrals. We show that the computational cost scales as Nlog{(N) rather than N squared, where NN is the number of discretization points. We test the method and its properties for a variety of vortex configurations, ranging from simple vortex rings to a counterflow vortex tangle, and compare results against the Local Induction Approximation and the exact Biot-Savart law.Comment: 12 pages, 10 figure

    Estimating habitat extent and carbon loss from an eroded northern blanket bog using UAV derived imagery and topography

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    Peatlands are important reserves of terrestrial carbon and biodiversity, and given that many peatlands across the UK and Europe exist in a degraded state, their conservation is a major area of concern and a focus of considerable research. Aerial surveys are valuable tools for habitat mapping and conservation and provide useful insights into their condition. We investigate how SfM photogrammetry-derived topography and habitat classes may be used to construct an estimate of carbon loss from erosion features in a remote blanket bog habitat. An autonomous, unmanned, aerial, fixed-wing remote sensing platform (Quest UAV 300™) collected imagery over Moor House, in the Upper Teesdale National Nature Reserve, a site with a high degree of peatland erosion. The images were used to generate point clouds into orthomosaics and digital surface models using SfM photogrammetry techniques, georeferenced and subsequently used to classify vegetation and peatland features. A classification of peatbog feature types was developed using a random forest classification model trained on field survey data and applied to UAV-captured products including the orthomosaic, digital surface model and derived surfaces such as topographic index, slope and aspect maps. Using the area classified as eroded peat and the derived digital surface model, we estimated a loss of 438 tonnes of carbon from a single gully. The UAV system was relatively straightforward to deploy in such a remote and unimproved area. SfM photogrammetry, imagery and random forest modelling obtained classification accuracies of between 42% and 100%, and was able to discern between bare peat, saturated bog and sphagnum habitats. This paper shows what can be achieved with low-cost UAVs equipped with consumer grade camera equipment and relatively straightforward ground control, and demonstrates their potential for the carbon and peatland conservation research community

    Optical types of inland and coastal waters

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    Inland and coastal waterbodies are critical components of the global biosphere. Timely monitoring is necessary to enhance our understanding of their functions, the drivers impacting on these functions and to deliver more effective management. The ability to observe waterbodies from space has led to Earth observation (EO) becoming established as an important source of information on water quality and ecosystem condition. However, progress toward a globally valid EO approach is still largely hampered by inconsistences over temporally and spatially variable in-water optical conditions. In this study, a comprehensive dataset from more than 250 aquatic systems, representing a wide range of conditions, was analyzed in order to develop a typology of optical water types (OWTs) for inland and coastal waters. We introduce a novel approach for clustering in situ hyperspectral water reflectance measurements (n = 4045) from multiple sources based on a functional data analysis. The resulting classification algorithm identified 13 spectrally distinct clusters of measurements in inland waters, and a further nine clusters from the marine environment. The distinction and characterization of OWTs was supported by the availability of a wide range of coincident data on biogeochemical and inherent optical properties from inland waters. Phylogenetic trees based on the shapes of cluster means were constructed to identify similarities among the derived clusters with respect to spectral diversity. This typification provides a valuable framework for a globally applicable EO scheme and the design of future EO missions
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