355 research outputs found
Polyandry in nature: a global analysis
This is the final version. Available on open access from Elsevier via the DOI in this recordA popular notion in sexual selection is that females are polyandrous and their offspring are commonly sired by more than a single male. We now have large-scale evidence from natural populations to be able to verify this assumption. Although we concur that polyandry is a generally common and ubiquitous phenomenon, we emphasise that it remains variable. In particular, the persistence of single paternity, both within and between populations, requires more careful consideration. We also explore an intriguing relation of polyandry with latitude. Several recent large-scale analyses of the relations between key population fitness variables, such as heterozygosity, effective population size (Ne), and inbreeding coefficients, make it possible to examine the global effects of polyandry on population fitness for the first time.Natural Environment Research Council (NERC
Sexual selection and the benefits of mating with attractive males in Drosophila simulans
Over the last century, sexual selection has grown from a controversial theory into a vast field of theoretical and empirical research. Although Darwin outlined two major mechanisms within his theory, male-male competition and female mate choice, the latter has promoted a wealth of research by virtue of its complexity. Despite decades of research into how female preferences and sexually selected traits have evolved, there is still little consensus as to why females prefer the males they do. Preferences are thought to evolve from either direct selection on the preference, as females themselves benefit directly from mating with a preferred male, or through indirect selection on the preference via offspring fitness. In all cases however, female preferences should compensate for the costs of discriminating between potential mates, if they are to remain overall beneficial. The fitness benefits of mating with preferred males were investigated here using the fruitfly Drosophila simulans, employing a range of behavioural, phenotypic and quantitative genetic approaches. The findings presented here indicate that female Drosophila simulans do not gain directly from mating with a preferred male. Multiple mating can increase fecundity, although costs from male harassment can reduce the net benefit. They also indicate that females may benefit indirectly from mating with attractive males as attractiveness is heritable and sons of preferred males are themselves preferred. There is also evidence that attractive males are successful in both the pre- and post-copulatory sense, as preferred males are better sperm competitors than less-preferred males. However, although there appear to be benefits from preferred males via their sons, there appear to be no benefits from males via their daughters’ fitness. These findings collectively indicate that female preferences in Drosophila simulans are driven by indirectly selected benefits (via Fisherian sons), and that females benefit directly from mating multiply.The European Social Fun
Development of Nanoscale Metal-Organic Frameworks and Hybrid Silica Nanoparticles for Biomedical Applications
This dissertation describes the use of several different types of nanoparticles for biomedical applications including imaging, drug delivery, and sensing. Several novel contrast agents for magnetic resonance imaging have been developed using different nanoparticle platforms. First, highly efficient T1-weighted contrast agents were developed using mesoporous silica nanoparticles. This system was also extended to form a biodegradable contrast agent based on the same mesoporous silica nanoparticle platform. By incorporating paramagnetic metal ions Gd3+ and Mn2+ into nanoscale metal-organic frameworks, new contrast agents for magnetic resonance imaging have also been developed. All of these systems can also be modified to include either an organic fluorophore or luminescent lanthanide ion, forming multimodal contrast agents that can be used for both optical and magnetic resonance imaging. The use of nanoscale metal-organic frameworks for drug delivery will also be presented. Highly porous nanoscale metal-organic frameworks were synthesized using Fe 3+ and an organic bridging ligand. After the nanoparticles were synthesized, a platinum based anticancer drug was covalently attached to the porous framework. These nanoparticles have also been functionalized with organic fluorophores to create optical contrast agents. Finally, the use of a nanoparticle sensor for the detection of dipicolinic acid, a chemical marker for bacterial endospores (such as Anthrax), is demonstrated. Several different nanoparticle sensors were developed, and they are advantageous over the molecular complexes because they allow for the incorporation of an internal standard, which eliminates the need for instrument specific calibration curves
Can patterns of chromosome inversions in Drosophila pseudoobscura predict polyandry across a geographical cline?
Journal ArticleFemale multiple mating, known as polyandry, is ubiquitous and occurs in a wide variety of taxa. Polyandry varies greatly from species in which females mate with one or two males in their lifetime to species in which females may mate with several different males on the same day. As multiple mating by females is associated with costs, numerous hypotheses attempt to explain this phenomenon. One hypothesis not extensively explored is the possibility that polyandrous behavior is captured and "fixed" in populations via genetic processes that preserve the behavior independently of any adaptive benefit of polyandry. Here, we use female isolines derived from populations of Drosophila pseudoobscura from three locations in North America to examine whether different female remating levels are associated with patterns of chromosome inversions, which may explain patterns of polyandry across the geographic range. Populations differed with respect to the frequency of polyandry and the presence of inversion polymorphisms on the third chromosome. The population with the lowest level of female remating was the only one that was entirely comprised of homokaryotypic lines, but the small number of populations prevented us investigating this relationship further at a population level. However, we found no strong relationship between female remating levels and specific karyotypes of the various isolines.NER
Opposite environmental and genetic influences on body size in North American Drosophila pseudoobscura.
Journal ArticleResearch Support, Non-U.S. Gov'tBACKGROUND: Populations of a species often differ in key traits. However, it is rarely known whether these differences are associated with genetic variation and evolved differences between populations, or are instead simply a plastic response to environmental differences experienced by the populations. Here we examine the interplay of plasticity and direct genetic control by investigating temperature-size relationships in populations of Drosophila pseudoobscura from North America. We used 27 isolines from three populations and exposed them to four temperature regimes (16°C, 20°C, 23°C, 26°C) to examine environmental, genetic and genotype-by-environment sources of variance in wing size. RESULTS: By far the largest contribution to variation in wing size came from rearing temperature, with the largest flies emerging from the coolest temperatures. However, we also found a genetic signature that was counter to this pattern as flies originating from the northern, cooler population were consistently smaller than conspecifics from more southern, warmer populations when reared under the same laboratory conditions. CONCLUSIONS: We conclude that local selection on body size appears to be acting counter to the environmental effect of temperature. We find no evidence that local adaptation in phenotypic plasticity can explain this result, and suggest indirect selection on traits closely linked with body size, or patterns of chromosome inversion may instead be driving this relationship.NERCBBSR
Temperature can shape a cline in polyandry, but only genetic variation can sustain it over time
Multiple mating by females (polyandry) is a widespread behavior occurring in diverse taxa, species, and populations. Polyandry can also vary widely within species, and individual populations, so that both monandrous and polyandrous females occur together. Genetic differences can explain some of this intraspecific variation in polyandry, but environmental factors are also likely to play a role. One environmental factor that influences many fundamental biological processes is temperature. Higher temperatures have been shown to directly increase re-mating in laboratory studies of insects. In the longer term, high temperature could also help to drive the evolution of larger-scale patterns of behavior by changing the context-dependent balance of costs and benefits of polyandry across environments. We examined the relative influence of rearing and mating temperature on female re-mating in populations of Drosophila pseudoobscura that show a latitudinal cline in polyandry in nature, using a range of ecologically relevant temperatures. We found that females of all genotypes re-mated more at cooler temperatures, which fits with the observation of higher average frequencies of polyandry at higher latitudes in this species. However, the impact of temperature was outweighed by the strong genetic control of re-mating in females in this species. It is likely that genetic factors provide the primary explanation for the latitudinal cline in polyandry in this species.NER
Using Threshold Concepts to generate a new understanding of teaching and learning Biology
Students come to tertiary institutions with misconceptions of key concepts in the disciplines they are studying. Their misconceptions commonly relate to conceptually difficult or troublesome knowledge (Perkins 1999) and can be: incomplete, contradictory, stable and highly resistant to change and remain intact despite repeated instruction at successively higher levels, being perhaps reinforced by teachers and textbooks (Driver 1983; Driver, Guesne and Tiberghien, 1985; Gabel 1994). For sometime, we have known that a range of concepts in Biology are conceptually difficult e.g. biochemical pathways, evolution and genetics (Brown 1995; Ross and Tronson 2007, Taylor 2006, 2008), but whether these are the ‘threshold concepts’ of (Meyer and Land 1995) is a question that needs to be explored further. We propose an alternative perspective where threshold crossing can be envisaged more productively as a cognitive process with students transported across a conceptual chasm or threshold. Misconceptions may then lie with an underlying ‘cognitive threshold’ and not a ‘threshold concept’ (Ross et al 2008). This current ALTC funded collaborative project involves three Australian universities and aims to identify the cognitive processes which underlie difficult Biological concepts; develop intervention strategies to improve students’ framework of conceptual understanding, in one or more related concept areas (that is, to help the students cross a conceptual threshold); test whether students can subsequently transfer this thinking process to aid their understanding of other similarly difficult concepts (that is, to see if they have learnt how to cross unfamiliar thresholds). In this paper we present the preliminary results of a survey which asked biology academics (both nationally and internationally) to identify troublesome biological concepts in their teaching, describe the cognitive process that underlies them which may determine why they are troublesome, and to identify the links they perceive with our nominated cognitive thresholds
Bamboozled! Resolving deep evolutionary nodes within the phylogeny of bamboo corals (Octocorallia: Scleralcyonacea: Keratoisididae).
Keratoisididae is a globally distributed, and exclusively deep-sea, family of octocorals that contains species and genera that are polyphyletic. An alphanumeric system, based on a three-gene-region phylogeny, is widely used to describe the biodiversity within this family. That phylogeny identified 12 major groups although it did not have enough signal to explore the relationships among groups. Using increased phylogenomic resolution generated from Ultraconserved Elements and exons (i.e. conserved elements), we aim to resolve deeper nodes within the family and investigate the relationships among those predefined groups. In total, 109 libraries of conserved elements were generated from individuals representing both the genetic and morphological diversity of our keratoisidids. In addition, the conserved element data of 12 individuals from previous studies were included. Our taxon sampling included 11 of the 12 keratoisidid groups. We present two phylogenies, constructed from a 75% (231 loci) and 50% (1729 loci) taxon occupancy matrix respectively, using both Maximum Likelihood and Multiple Species Coalescence methods. These trees were congruent at deep nodes. As expected, S1 keratoisidids were recovered as a well-supported sister clade to the rest of the bamboo corals. S1 corals do not share the same mitochondrial gene arrangement found in other members of Keratoisididae. All other bamboo corals were recovered within two major clades. Clade I comprises individuals assigned to alphanumeric groups B1, C1, D1&D2, F1, H1, I4, and J3 while Clade II contains representatives from A1, I1, and M1. By combining genomics with already published morphological data, we provide evidence that group H1 is not monophyletic, and that the division between other groups - D1 and D2, and A1 and M1 - needs to be reconsidered. Overall, there is a lack of robust morphological markers within Keratoisididae, but subtle characters such as sclerite microstructure and ornamentation seem to be shared within groups and warrant further investigation as taxonomically diagnostic characters
Effects of antiplatelet therapy on stroke risk by brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases: subgroup analyses of the RESTART randomised, open-label trial
Background
Findings from the RESTART trial suggest that starting antiplatelet therapy might reduce the risk of recurrent symptomatic intracerebral haemorrhage compared with avoiding antiplatelet therapy. Brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases (such as cerebral microbleeds) are associated with greater risks of recurrent intracerebral haemorrhage. We did subgroup analyses of the RESTART trial to explore whether these brain imaging features modify the effects of antiplatelet therapy
The Long-Baseline Neutrino Experiment: Exploring Fundamental Symmetries of the Universe
The preponderance of matter over antimatter in the early Universe, the
dynamics of the supernova bursts that produced the heavy elements necessary for
life and whether protons eventually decay --- these mysteries at the forefront
of particle physics and astrophysics are key to understanding the early
evolution of our Universe, its current state and its eventual fate. The
Long-Baseline Neutrino Experiment (LBNE) represents an extensively developed
plan for a world-class experiment dedicated to addressing these questions. LBNE
is conceived around three central components: (1) a new, high-intensity
neutrino source generated from a megawatt-class proton accelerator at Fermi
National Accelerator Laboratory, (2) a near neutrino detector just downstream
of the source, and (3) a massive liquid argon time-projection chamber deployed
as a far detector deep underground at the Sanford Underground Research
Facility. This facility, located at the site of the former Homestake Mine in
Lead, South Dakota, is approximately 1,300 km from the neutrino source at
Fermilab -- a distance (baseline) that delivers optimal sensitivity to neutrino
charge-parity symmetry violation and mass ordering effects. This ambitious yet
cost-effective design incorporates scalability and flexibility and can
accommodate a variety of upgrades and contributions. With its exceptional
combination of experimental configuration, technical capabilities, and
potential for transformative discoveries, LBNE promises to be a vital facility
for the field of particle physics worldwide, providing physicists from around
the globe with opportunities to collaborate in a twenty to thirty year program
of exciting science. In this document we provide a comprehensive overview of
LBNE's scientific objectives, its place in the landscape of neutrino physics
worldwide, the technologies it will incorporate and the capabilities it will
possess.Comment: Major update of previous version. This is the reference document for
LBNE science program and current status. Chapters 1, 3, and 9 provide a
comprehensive overview of LBNE's scientific objectives, its place in the
landscape of neutrino physics worldwide, the technologies it will incorporate
and the capabilities it will possess. 288 pages, 116 figure
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