Extensive Genome Rearrangements of Caulobacter K31 and Genomic Diversity of type B3 Bacteriophages of Caulobacter Crescentus

The genus Caulobacter is found in a variety of habitats and is known for its ability to thrive in low-nutrient conditions. K31 is a novel Caulobacter isolate that has the ability to tolerate copper and chlorophenols, and can grow at 48°C with a doubling time of 40 h. K31 contains a 5.5 Mb chromosome that codes for more than 5500 proteins and two large plasmids (234 and 178 kb) that code for 438 additional proteins. A comparison of the K31 genome and the Caulobacter crescentus NA1000 genome, the representative strain and most well studied isolate of the Caulobacter genus, revealed extensive rearrangements of gene order suggesting that the genomes had been randomly scrambled. However, a careful analysis revealed that the distance from the origin of replication was conserved for the majority of the genes and that many of the rearrangements involved inversions that included the origin of replication. On a finer scale, numerous small indels were observed. K31 proteins involved in essential functions shared 80 – 95% amino acid sequence identity with their C. crescentus homologues, while other homologue pairs tended to have lower levels of identity. In addition, the K31 chromosome contains more than 1600 genes with no homologue in NA1000. The genomes of type B3 bacteriophage of Caulobacter crescentus are among the largest phage genomes thus far deposited into GenBank with sizes over 200 kb. The bacteriophage samples of our collection where first isolated by graduate students of Dr. Ely’s lab in 1977 in an project aimed at discovering transducing bacteriophages of Caulobacter crescentus (Johnson 1977). We began our genomic characterization of these bacteriophages in hopes of finding genomic rearrangements as observed in the host NA1000 and possibly a more clear understanding of the phenomenon. However, no major rearrangements were discovered and we changed our direction to be more of an evolutionary analysis of this group of bacteriophages. We introduce six new bacteriophage genomes which were obtained from phage collected from various water systems in the southeastern United States and from tropical locations across the globe. Evolutionary analysis of the genomes reveal a “core genome” which accounts for roughly 1/3 of the bacteriophage genomes and is predominately localized to the head, tail, and lysis gene regions. Despite being isolated from geographically distinct locations, the genomes of these bacteriophages were highly conserved in genome sequence and gene order. Here we present the results of our analysis which identify the insertions, deletions, translocations, and horizontal gene transfer events which are responsible for the genomic diversity of this group of bacteriophages

Origin Of Current-Induced Forces In An Atomic Gold Wire: A First Principles Study

We address the microscopic origin of the current-induced forces by analyzing results of first principles density functional calculations of atomic gold wires connected to two gold electrodes with different electrochemical potentials. We find that current induced forces are closely related to the chemical bonding, and arise from the rearrangement of bond charge due to the current flow. We explain the current induced bond weakening/strengthening by introducing bond charges decomposed into electrode components.Comment: 4 pages, 4 figure

Aqueous, QUick-Charging Battery Integration For Electric Flight Research

AQUIFER establishes technical feasibility of an early-stage technology, a high-energy density, aqueous-based, flow battery, resulting in a near-term increase of 1.7 times range over an all-electric battery, while retiring fire and explosion hazards associated with lithium-based chemistries. The Nano-electrofuel (NEF) flow battery will be integrated with a rim-driven motor (RDM) as a multi-functional design to eliminate conductive EMI and weight from long cable runs, and provide liquid cooling from the aqueous fuel. When successful, the technology provides an improved safety energy storage solution for emission-free electric propulsion in commercial aviation

Conductance switching in a molecular device: the role of sidegroups and intermolecular interactio

We report first-principles studies of electronic transport in monolayers of Tour wires functionalized with different side groups. An analysis of the scattering states and transmission eigenchannels suggests that the functionalization does not strongly affect the resonances responsible for current flow through the monolayer. However, functionalization has a significant effect on the interactions within the monolayer, so that monolayers with NO$_2$ side groups exhibit local minima associated with twisted conformations of the molecules. We use our results to interpret observations of negative differential resistance and molecular memory in monolayers of NO$_2$ functionalized molecules in terms of a twisting of the central ring induced by an applied bias potential.Comment: 4 pages, 4 figure

Differential Uptake of Gold Nanoparticles by 2 Species of Tadpole, the Wood Frog (Lithobates Sylvaticus) and the Bullfrog (Lithobates Catesbeianus)

Engineered nanoparticles are aquatic contaminants of emerging concern that exert ecotoxicological effects on a wide variety of organisms. We exposed cetyltrimethylammonium bromide–capped spherical gold nanoparticles to wood frog and bullfrog tadpoles with conspecifics and in combination with the other species continuously for 21 d, then measured uptake and localization of gold. Wood frog tadpoles alone and in combination with bullfrog tadpoles took up significantly more gold than bullfrogs. Bullfrog tadpoles in combination with wood frogs took up significantly more gold than controls. The rank order of weight-normalized gold uptake was wood frogs in combination \u3e wood frogs alone \u3e bullfrogs in combination \u3e bullfrogs alone \u3e controls. In all gold-exposed groups of tadpoles, gold was concentrated in the anterior region compared with the posterior region of the body. The concentration of gold nanoparticles in the anterior region of wood frogs both alone and in combination with bullfrogs was significantly higher than the corresponding posterior regions. We also measured depuration time of gold in wood frogs. After 21 d in a solution of gold nanoparticles, tadpoles lost \u3e83% of internalized gold when placed in gold-free water for 5 d. After 10 d in gold-free water, tadpoles lost 94% of their gold. After 15 d, gold concentrations were below the level of detection. Our finding of differential uptake between closely related species living in similar habitats with overlapping geographical distributions argues against generalizing toxicological effects of nanoparticles for a large group of organisms based on measurements in only one species

A possible optical counterpart to the old nearby pulsar J0108-1431

The multi-wavelength study of old (>100 Myr) radio pulsars holds the key to understanding the long-term evolution of neutron stars, including the advanced stages of neutron star cooling and the evolution of the magnetosphere. Optical/UV observations are particularly useful for such studies because they allow one to explore both thermal and non-thermal emission processes. In particular, studying the optical/UV emission constrains temperature of the bulk of the neutron star surface, too cold to be measured in X-ray observations.Aim of this work is to identify the optical counterpart of the very old (166 Myr) radio pulsar J0108-1431. We have re-analyzed our original VLT observations (Mignani et al. 2003), where a very faint object was tentatively detected close to the radio position, near the edge of a field galaxy. We found that the backward extrapolation of the PSR J0108-1431 proper motion recently measured by CHANDRA(Pavlov et al. 2008) nicely fits the position of this object. Based on that, we propose it as a viable candidate for the optical counterpart to PSR J0108-1431. The object fluxes (U =26.4+/-0.3; B =27.9; V >27.8) are consistent with a thermal spectrum with a brightness temperature of 9X10^4 K (for R = 13 km at a distance of 130 pc), emitted from the bulk of the neutron star surface. New optical observations are required to confirm the optical identification of PSR J0108-1431 and measure its spectrum.Comment: 5 pages, 4 figures, submitted to A&

Status Report on the Application of Integrated Approaches to Water Resources Management 2012

UN-Water produced a status report regarding the progress of water resources management in several countries as well as possible outcomes and impacts of integrated approaches. It is also based on a 2011 UN-Water survey sent to the governments of all UN member states and a series of complementary interviews in 30 representative countries