32 research outputs found
Bacterias de suelo solubilizadoras de fosfato y su interacción con plantas de interés agronómico.
Synthetic Communities of Bacterial Endophytes to Improve the Quality and Yield of Legume Crops
Plant-associated microbiomes confer fitness advantages to the plant host by growth promotion through different mechanisms including nutrient uptake, phytohormones production, resistance to pathogens, and stress tolerance. These effects of the potentially beneficial microbes have been used in a diversity of biotechnological approaches to improve crop performance applying individual bacterial cultures. However, healthy plants host a diversity of microorganisms (microbiota). Next-generation sequencing technologies have offered insights into
the relative abundances of different phylogenetic groups in a community and the metabolic and physiological potential of its members. In the last decade, researchers have started to explore the possibilities to use temporal and functional combinations of those bacteria in the form of synthetic communities. In this chapter, we review
the benefits of using endophytic bacteria in legumes, the available methodological approaches to study the effects of bacterial communities, and the most recent findings using synthetic communities to improve the performance of legume crops.Instituto de PatologĂa VegetalFil: Monteoliva, Mariela Ines. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de FisiologĂa y Recursos GenĂ©ticos Vegetales; ArgentinaFil: Monteoliva, Mariela Ines. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Unidad de Estudios Agropecuarios (UDEA) ; ArgentinaFil: Valetti, Lucio. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de PatologĂa Vegetal; ArgentinaFil: Valetti, Lucio. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Unidad de FitopatologĂa y ModelizaciĂłn AgrĂcola (UFyMA); ArgentinaFil: Taurian, Tania. Universidad Nacional de RĂo Cuarto (UNRC). Facultad de Ciencias Exactas, FĂsico-QuĂmicas y Naturales. Departamento de Ciencias Naturales; ArgentinaFil: Taurian, Tania. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Instituto de Investigaciones AgrobiotecnolĂłgicas; ArgentinaFil: Crociara, Clara Sonia. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Unidad de FitopatologĂa y ModelizaciĂłn AgrĂcola (UFyMA); ArgentinaFil: Crociara, Clara Sonia. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de PatologĂa Vegetal; ArgentinaFil: Guzzo, MarĂa Carla. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de FisiologĂa y Recursos GenĂ©ticos Vegetales; ArgentinaFil: Guzzo, MarĂa Carla. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentin
Peanut Endophytic Phosphate Solubilizing Bacteria Increase Growth and P Content of Soybean and Maize Plants
Phosphorus (P) is a limiting factor of plant development due to its low availability in the soil. The use of endophytic phosphate solubilizing bacteria as a more sustainable alternative to the use of chemical phosphorus fertilizers is proposed in this study. The objectives were to analyze the effect of simple inoculations of native peanut endophytic phosphate solubilizing bacteria on plant growth promotion and P content of soybean and maize and to evaluate their survival and endophytic colonization capacity on these plants. In addition, bacterial plant cell wall degrading enzymes activities in presence or absence of root exudates was determined. Soybean, maize and peanut plants were grown on a microcosm scale and inoculated with Enterobacter sp. J49 or Serratia sp. S119. It was observed that phosphate solubilizing strains promoted the growth of maize and soybean plants and contributed significantly P to their tissues. A significant increase in the phosphate solubilizing capacity of the plant rhizosphere after the end of the assay was observed. The strains showed to survive in plant’s growth substrate and in the case of Enterobacter sp. J49, it showed also to colonize endophytically maize and soybean. Root exudates of the three plants showed to produce changes in pectinase and cellulase activities of the strains. The bacterial strains analyzed in this study constitutes potential sources for the formulation of biofertilizers for their application for several crops in agricultural soils with low P content.Fil: Lucero, Cinthia Tamara. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; Argentina. Universidad Nacional de La Pampa; Argentina. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas, FisicoquĂmicas y Naturales. Departamento de Ciencias Naturales; ArgentinaFil: Lorda, Graciela Susana. Universidad Nacional de La Pampa; ArgentinaFil: Anzuay, MarĂa Soledad. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas, FisicoquĂmicas y Naturales. Departamento de Ciencias Naturales; Argentina. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Instituto de Investigaciones AgrobiotecnolĂłgicas. - Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Investigaciones AgrobiotecnolĂłgicas; ArgentinaFil: Ludueña, Liliana Mercedes. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas, FisicoquĂmicas y Naturales. Departamento de Ciencias Naturales; Argentina. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Instituto de Investigaciones AgrobiotecnolĂłgicas. - Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Investigaciones AgrobiotecnolĂłgicas; ArgentinaFil: Taurian, Tania. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Instituto de Investigaciones AgrobiotecnolĂłgicas. - Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Investigaciones AgrobiotecnolĂłgicas; Argentina. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas, FisicoquĂmicas y Naturales. Departamento de Ciencias Naturales; Argentin
Motility and biofilm production involved in the interaction of phosphate solubilizing endophytic strains with peanut, maize and soybean plants
Endophytic plant growth promoting bacteria are of great interest since the ability to colonize the internal tissues of plants gives it an adaptive advantage. To confer any beneficial effect on the plant, bacteria must present a successful colonization and thus be competent in the rhizosphere and finally infect internal tissues. In this sense, bacterial motility is an essential property involved in plant-microorganism interaction where bacteria can colonize the root and at the same time form biofilms. Within plant beneficial bacteria those that present phosphate solubilizing activity are of great interest due to the contribution of phosphorus to plants. The objective of this work was to evaluate the motility and biofilm formation properties of the strains Serratia sp. S119 and Enterobacter sp. J49 growing in the presence of peanut, maize and soybean root exudates. Results obtained indicated that both strains have flagella, possess swimming, swarming and twitching motilities and showed differential chemotactic attraction against root exudates. In addition, the strains under study showed the ability to form biofilms, being this ability greater in minimal media, in which a greater decrease was also seen by the addition of root exudates in the first hours of growth. The changes produced by the addition of root exudates in the chemotaxis and biofilm formation of the strains suggests that compounds released by the plants are detected by these bacteria and could be part of the molecular dialogue involved in their interaction with the roots of plant.Fil: Lucero, Cinthia Tamara. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de QuĂmica; ArgentinaFil: Lorda, Graciela Susana. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de QuĂmica; ArgentinaFil: Ludueña, Liliana Mercedes. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Instituto de Investigaciones AgrobiotecnolĂłgicas - Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Investigaciones AgrobiotecnolĂłgicas; ArgentinaFil: Anzuay, MarĂa Soledad. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Instituto de Investigaciones AgrobiotecnolĂłgicas - Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Investigaciones AgrobiotecnolĂłgicas; ArgentinaFil: Taurian, Tania. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Instituto de Investigaciones AgrobiotecnolĂłgicas - Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Investigaciones AgrobiotecnolĂłgicas; Argentin
Development and field evaluation of liquid inoculants with native Bradyrhizobial strains for peanut production
A critical process in the leguminous crops cycles is biological nitrogen fixation (BNF). Application of inoculants with N fixing bacteria is economically and environmentally favourable. The aim of this work was to select competitive native peanut microsymbionts, evaluate their survival in inoculant support and assess their impact on peanut (Arachis hypogaea L.) production under field conditions at CĂłrdoba province in Argentina. The efficient N fixing Bradyrhizobium sp. J-81 and Bradyrhizobium sp. J-237, previously obtained from peanut nodules in the region of Cordoba, Argentina, were evaluated. In microcosm assays, plants inoculated with these isolates demonstrated better symbiotic parameters than those inoculated with reference strains. Different bacterial growth media and inoculant stabiliser solutions were evaluated. Balanced medium and arabic gum stabilising solution had optimal bacterial growth and the highest bacterial concentration and viability, respectively. Inoculation with either inoculants resulted in 44% greater peanut pod yield at Pizarro compared to the non-inoculated plants, although no significant differences were found with respect to commercial inoculants treatments.La fixation biologique de l’Azote (FBA) est un processus important dans le cycle de vie des lĂ©gumineuses. L’application d’inoculum de bactĂ©ries fixatrices d’azote est favorable au double plan Ă©conomique et environnemental. Le but de cette Ă©tude Ă©tait de sĂ©lectionner des bactĂ©ries symbiotiques de l’arachide natives et compĂ©titives, Ă©valuer leur temps de survie dans support d’inoculum et Ă©valuer leur impact sur la production en plein champ de l’arachide (Arachis hypogaea L.) dans la province de CĂłrdoba en Argentine. Les bactĂ©ries fixatrices d’azote Bradyrhizobium sp. J-81 et Bradyrhizobium sp. J-237, extraites de nodules collectĂ©s sur des plants cultivĂ©s dans la rĂ©gion de Cordoba en Argentina, ont Ă©tĂ© Ă©valuĂ©es. Dans des essais de microcosme, des plants inoculĂ©s avec ces isolats ont exhibĂ©s de meilleurs paramètres symbiotiques que les plants non inoculĂ©s. DiffĂ©rents mĂ©dia de culture bactĂ©rienne et supports inoculums ont Ă©tĂ© testĂ©s. Medium mixte et solution stabilisĂ©e Ă la gomme arabique ont respectivement exhibĂ©s la croissance optimale des bactĂ©ries et la meilleure conservation et viabilitĂ© des bactĂ©ries. L’application de n’importe quel inoculum produisit 44% plus de rendement en gousses d’arachides Ă Pizarro par rapport aux plantes non-inoculĂ©es, et ceci bien qu’aucune diffĂ©rence significative n’a Ă©tĂ© observĂ©e en comparaison avec les traitements Ă l’inoculum du commerce.Fil: Valetti, Lucio. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Biologia General; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba; ArgentinaFil: Angelini, Jorge Guillermo. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Biologia General; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba; ArgentinaFil: Taurian, Tania. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Biologia General; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba; ArgentinaFil: Ibañez, Fernando Julio. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Biologia General; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba; ArgentinaFil: Muñoz, Vanina Laura. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Biologia General; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba; ArgentinaFil: Anzuay, MarĂa Soledad. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba; Argentina. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Biologia General; ArgentinaFil: Ludueña, Liliana Mercedes. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Biologia General; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba; ArgentinaFil: Fabra, Adriana Isidora. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Biologia General; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba; Argentin
Participation of type VI secretion system in plant colonization of phosphate solubilizing bacteria
In mutualistic endophytic bacteria, the type VI secretion system (T6SS) is related to important functions, such as interbacterial competition, stress response, quorum sensing, biofilm formation, and symbiosis. The presence of T6SS in beneficial endophytic bacterial population associated with different plants suggests that it plays an important role in its interaction with the eucaryotic partner. Within plant promoting bacteria, those with phosphate solubilizing activity constitute a group of great relevance to the rhizosphere as they provide phosphorus to plants. Among them, those with endophytic colonization capacity have survival advantages. The aim of this study was to determine whether the T6SS of a native peanut phosphate solubilizing bacterium is involved in its colonization in this legume. Initially, an in silico analysis looking for genes related to T6SS in the genome of the Enterobacter sp. J49 strain enabled us to identify almost all the tss genes, except for the tssE gene. A T6SS mutant of the Enterobacter sp. J49 strain was obtained by interrupting one of the essential tss genes. Then, the Enterobacter sp. J49-hcp strain was inoculated on peanut plants to analyze its colonization capacity. In addition, properties associated with endophytic colonization were analyzed, such as the formation of biofilms and the production of pectinase and cellulase enzymes. The results obtained indicated a significant decrease in the epiphytic and endophytic colonization of the mutant with respect to the wild strain. It is possible to conclude that T6SS, although not essential, may participate in bacterial colonization, either by accelerating the infection or by promoting other mechanisms involved in it.Fil: Lucero, Cinthia Tamara. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de QuĂmica; ArgentinaFil: Lorda, Graciela Susana. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de QuĂmica; ArgentinaFil: Ludueña, Liliana Mercedes. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; ArgentinaFil: Nievas, Fiorela Lujan. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Departamento de BiologĂa Molecular; ArgentinaFil: Bogino, Pablo Cesar. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Departamento de BiologĂa Molecular; ArgentinaFil: Angelini, Jorge Guillermo. Universidad Nacional de RĂo Cuarto. Facultad de Ciencias Exactas FisicoquĂmicas y Naturales. Instituto de Investigaciones AgrobiotecnolĂłgicas. - Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Investigaciones AgrobiotecnolĂłgicas; ArgentinaFil: Ambrosino, Mariela Lis. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de QuĂmica; ArgentinaFil: Taurian, Tania. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentin
Native bacteria isolated from roots and rhizosphere of Solanum lycopersicum L. increase tomato seedling growth under a reduced fertilization regime
In semiarid regions is important to use native strains best adapted to these environments to optimize plant-PGPR interaction. We aimed to isolate and characterize PGPR from roots and rhizosphere of a tomato crop, as well as studying the effect of its inoculation on tomato seedlings growth. We selected four strains considering their effectiveness of fixing nitrogen, solubilizing phosphate, producing siderophores and indole acetic acid. They belong to the genera Enterobacter, Pseudomonas, Cellulosimicrobium, and Ochrobactrum. In addition, we also analyzed the ability to solubilize Ca3(PO4)2, FePO4 and AlPO4 and the presence of one of the genes encoding the cofactor PQQ in their genome. Enterobacter 64S1 and Pseudomonas 42P4 showed the highest phosphorus solubilizing activity and presence of pqqE gene. Furthermore, in a tomato-based bioassay in speed-bed demonstrated that a sole inoculation at seedling stage with the strains increased dry weight of roots (49–88%) and shoots (39–55%), stem height (8–13%) and diameter (5–8%) and leaf area (22–31%) and were equal or even higher than fertilization treatment. Leaf nitrogen and chlorophyll levels were also increased (50–80% and 26–33%) compared to control. These results suggest that Enterobacter 64S1 and Pseudomonas 42P4 can be used as bio-inoculant in order to realize a nutrient integrated management.Fil: PĂ©rez Rodriguez, MarĂa Micaela. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Mendoza. Instituto de BiologĂa AgrĂcola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de BiologĂa AgrĂcola de Mendoza; ArgentinaFil: Piccoli, Patricia NoemĂ. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Mendoza. Instituto de BiologĂa AgrĂcola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de BiologĂa AgrĂcola de Mendoza; ArgentinaFil: Anzuay, MarĂa Soledad. Universidad Nacional de RĂo Cuarto; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Baraldi, Rita. Consiglio Nazionale delle Ricerche; ItaliaFil: Neri, Luisa. Consiglio Nazionale delle Ricerche; ItaliaFil: Taurian, Tania. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; Argentina. Universidad Nacional de RĂo Cuarto; ArgentinaFil: Lobato Ureche, Miguel AndrĂ©s. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Mendoza. Instituto de BiologĂa AgrĂcola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de BiologĂa AgrĂcola de Mendoza; ArgentinaFil: Segura, Diana MarĂa. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Mendoza. Instituto de BiologĂa AgrĂcola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de BiologĂa AgrĂcola de Mendoza; ArgentinaFil: Cohen, Ana Carmen. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Mendoza. Instituto de BiologĂa AgrĂcola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de BiologĂa AgrĂcola de Mendoza; Argentin