162 research outputs found
De-biasing interferometric visibilities in VLTI-AMBER data of low SNR observations
AIMS: We have found that the interferometric visibilities of VLTI-AMBER
observations, extracted via the standard reduction package, are significantly
biased when faint targets are concerned. The visibility biases derive from a
time variable fringing effect (correlated noise) appearing on the detector.
METHODS: We have developed a method to correct this bias that consists in a
subtraction of the extra power due to such correlated noise, so that the real
power spectrum at the spatial frequencies of the fringing artifact can be
restored. RESULTS: This pre-processing procedure is implemented in a software,
called AMDC and available to the community, to be run before the standard
reduction package. Results obtained on simulated and real observations are
presented and discussed.Comment: 7 pages, 9 figure
AMBER on the VLTI: data processing and calibration issues
We present here the current performances of the AMBER / VLTI instrument for
standard use and compare these with the offered modes of the instrument. We
show that the instrument is able to reach its specified precision only for
medium and high spectral resolution modes, differential observables and bright
objects. For absolute observables, the current achievable accuracy is strongly
limited by the vibrations of the Unit Telescopes, and also by the observing
procedure which does not take into account the night-long transfer function
monitoring. For low-resolution mode, the current limitation is more in the data
reduction side, since several effects negligible at medium spectral resolution
are not taken into account in the current pipeline. Finally, for faint objects
(SNR around 1 per spectral channel), electromagnetic interferences in the VLTI
interferometric laboratory with the detector electronics prevents currently to
get unbiased measurements. Ideas are under study to correct in the data
processing side this effect, but a hardware fix should be investigated
seriously since it limits seriously the effective limiting magnitude of the
instrument.Comment: 10 page
Mitochondrial Hormesis links nutrient restriction to improved metabolism in fat cell
Fasting promotes longevity by reprogramming metabolic and stress resistance pathways. However, although the impact on adipose tissue physiology through hormonal inputs is well established, the direct role of fasting on adipose cells is poorly understood. Herein we show that white and beige adipocytes, as well as mouse epididymal and subcutaneous adipose depots, respond to nutrient scarcity by acquiring a brown-like phenotype. Indeed, they improve oxidative metabolism through modulating the expression of mitochondrial- and nuclear-encoded oxidative phosphorylation genes as well as mitochondrial stress defensive proteins (UCP1, SOD2). Such adaptation is placed in a canonical mitohormetic response that proceeds via mitochondrial reactive oxygen species ((mt)ROS) production and redistribution of FoxO1 transcription factor into nucleus. Nuclear FoxO1 ((n)FoxO1) mediates retrograde communication by inducing the expression of mitochondrial oxidative and stress defensive genes. Collectively, our findings describe an unusual white/beige fat cell response to nutrient availability highlighting another health-promoting mechanism of fasting
First astrophysical results from AMBER/VLTI
The AMBER instrument installed at the Very Large Telescope (VLT) combines
three beams from as many telescopes to produce spectrally dispersed fringes
from milli-arcsecond angular scale in the near infrared. Two years after
installation, first scientific observations have been carried out during the
Science Demonstration Time and the Guaranteed Time mostly on bright sources due
to some VLTI limitations. In this paper, we review these first astrophysical
results and we show which types of completely new information is brought by
AMBER. The first astrophysical results have been mainly focusing on stellar
wind structure, kinematics, and its interaction with dust usually concentrated
in a disk. Because AMBER has dramatically increased the number of measures per
baseline, this instrument brings strong constraints on morphology and models
despite a relatively poor (u, v) coverage for each object.Comment: SPIE 6268 proceeding
Near-infrared interferometric observation of the Herbig Ae star HD144432 with VLTI/AMBER
We study the sub-AU-scale circumstellar environment of the Herbig Ae star
HD144432 with near-infrared (NIR) VLTI/AMBER observations to investigate the
structure of its inner dust disk. The interferometric observations were carried
out with the AMBER instrument in the H and K band. We interpret the measured H-
and K-band visibilities, the near- and mid-infrared visibilities from the
literature, and the SED of HD144432 by using geometric ring models and
ring-shaped temperature-gradient disk models with power-law temperature
distributions. We derived a K-band ring-fit radius of 0.17 \pm 0.01 AU and an
H-band radius of 0.18 \pm 0.01 AU (for a distance of 145 pc). This measured
K-band radius of \sim0.17 AU lies in the range between the dust sublimation
radius of \sim0.13 AU (predicted for a dust sublimation temperature of 1500 K
and gray dust) and the prediction of models including backwarming (\sim0.27
AU). We found that an additional extended halo component is required in both
the geometric and temperature-gradient modeling. In the best temperature-
gradient model, the disk consists of two components. The inner part of the disk
is a thin ring with an inner radius of \sim0.21 AU, a temperature of \sim1600
K, and a ring thickness \sim0.02 AU. The outer part extends from \sim1 AU to
\sim10 AU with an inner temperature of \sim400 K. We find that the disk is
nearly face-on with an inclination angle of < 28 degree. Our
temperature-gradient modeling suggests that the NIR excess is dominated by
emission from a narrow, bright rim located at the dust sublimation radius,
while an extended halo component contributes \sim6% to the total flux at 2
{\mu}m. The MIR model emission has a two-component structure with \sim20% flux
from the inner ring and the rest from the outer part. This two-component
structure suggests a disk gap, which is possibly caused by the shadow of a
puffed-up inner rim.Comment: 7 pages, 5 figures, accepted by A&
Data reduction for the AMBER instrument
We present here the general formalism and data processing steps used in the data reduction pipeline of the AMBER instrument. AMBER is a three-telescope interferometric beam combiner in J, H and K bands installed at ESO\'s Very Large Telescope Interferometer. The fringes obtained on the 3 pairs of telescopes are spatially coded and spectrally dispersed. These are monitored on a 512x512 infrared camera at frame rates up to 100 frames per second, and this paper presents the algorithm used to retrieve the complex coherent visibility of the science target and the subsequent squared visibility, dierential phase and phase closure on the 3 bases and in the 3 spectral bands available in AMBER
Glutathione Decrement Drives Thermogenic Program in Adipose Cells
Adipose tissue metabolically adapts to external stimuli. We demonstrate that the induction of the thermogenic program in white adipocytes, through cold exposure in mice or in vitro adrenergic stimulation, is accompanied by a decrease in the intracellular content of glutathione (GSH). Moreover, the treatment with a GSH depleting agent, buthionine sulfoximine (BSO), recapitulates the effect of cold exposure resulting in the induction of thermogenic program. In particular, BSO treatment leads to enhanced uncoupling respiration as demonstrated by increased expression of thermogenic genes (e.g. Ucp1, Ppargc1a), augmented oxygen consumption and decreased mitochondrial transmembrane potential. Buffering GSH decrement by pre-treatment with GSH ester prevents the up-regulation of typical markers of uncoupling respiration. We demonstrate that FoxO1 activation is responsible for the conversion of white adipocytes into a brown phenotype as the "browning" effects of BSO are completely abrogated in cells down-regulating FoxO1. In mice, the BSO-mediated up-regulation of uncoupling genes results in weight loss that is at least in part ascribed to adipose tissue mass reduction. The induction of thermogenic program has been largely proposed to counteract obesity-related diseases. Based on these findings, we propose GSH as a novel therapeutic target to increase energy expenditure in adipocytes.Adipose tissue metabolically adapts to external stimuli. We demonstrate that the induction of the thermogenic program in white adipocytes, through cold exposure in mice or in vitro adrenergic stimulation, is accompanied by a decrease in the intracellular content of glutathione (GSH). Moreover, the treatment with a GSH depleting agent, buthionine sulfoximine (BSO), recapitulates the effect of cold exposure resulting in the induction of thermogenic program. In particular, BSO treatment leads to enhanced uncoupling respiration as demonstrated by increased expression of thermogenic genes (e.g. Ucp1, Ppargc1a), augmented oxygen consumption and decreased mitochondrial transmembrane potential. Buffering GSH decrement by pre-treatment with GSH ester prevents the up-regulation of typical markers of uncoupling respiration. We demonstrate that FoxO1 activation is responsible for the conversion of white adipocytes into a brown phenotype as the "browning" effects of BSO are completely abrogated in cells down-regulating FoxO1. In mice, the BSO-mediated up-regulation of uncoupling genes results in weight loss that is at least in part ascribed to adipose tissue mass reduction. The induction of thermogenic program has been largely proposed to counteract obesity-related diseases. Based on these findings, we propose GSH as a novel therapeutic target to increase energy expenditure in adipocytes
The 2008 outburst in the young stellar system ZCMa: I. Evidence of an enhanced bipolar wind on the AU-scale
Accretion is a fundamental process in star formation. Although the time
evolution of accretion remains a matter of debate, observations and modelling
studies suggest that episodic outbursts of strong accretion may dominate the
formation of the protostar. Observing young stellar objects during these
elevated accretion states is crucial to understanding the origin of unsteady
accretion. ZCMa is a pre-main-sequence binary system composed of an embedded
Herbig Be star, undergoing photometric outbursts, and a FU Orionis star. The
Herbig Be component recently underwent its largest optical photometric outburst
detected so far. We aim to constrain the origin of this outburst by studying
the emission region of the HI Brackett gamma line, a powerful tracer of
accretion/ejection processes on the AU-scale in young stars. Using the
AMBER/VLTI instrument at spectral resolutions of 1500 and 12 000, we performed
spatially and spectrally resolved interferometric observations of the hot gas
emitting across the Brackett gamma emission line, during and after the
outburst. From the visibilities and differential phases, we derive
characteristic sizes for the Brackett gamma emission and spectro-astrometric
measurements across the line, with respect to the continuum. We find that the
line profile, the astrometric signal, and the visibilities are inconsistent
with the signature of either a Keplerian disk or infall of matter. They are,
instead, evidence of a bipolar wind, maybe partly seen through a disk hole
inside the dust sublimation radius. The disappearance of the Brackett gamma
emission line after the outburst suggests that the outburst is related to a
period of strong mass loss rather than a change of the extinction along the
line of sight. Based on these conclusions, we speculate that the origin of the
outburst is an event of enhanced mass accretion, similar to those occuring in
EX Ors and FU Ors.Comment: Accepted for publication in Astronomy and Astrophysics Letter
Ellipsoidal primary of the RS CVn binary zeta And: Investigation using high-resolution spectroscopy and optical interferometry
We have obtained high-resolution spectroscopy, optical interferometry, and
long-term broad band photometry of the ellipsoidal primary of the RS CVn-type
binary system zeta And. Based on the optical interferometry the apparent limb
darkened diameter of zeta And is 2.55 +/- 0.09 mas using a uniform disk fit.
The Hipparcos distance and the limb-darkened diameter obtained with a uniform
disk fit give stellar radius of 15.9 +/- 0.8 Rsolar, and combined with
bolometric luminosity, it implies an effective temperature of 4665 +/- 140 K.
The temperature maps obtained from high resolution spectra using Doppler
imaging show a strong belt of equatorial spots and hints of a cool polar cap.
The equatorial spots show a concentration around the phase 0.75. This spot
configuration is reminiscent of the one seen in the earlier published
temperature maps of zeta And. Investigation of the Halpha line reveals both
prominences and cool clouds in the chromosphere. Long-term photometry spanning
12 years shows hints of a spot activity cycle, which is also implied by the
Doppler images, but the cycle length cannot be reliably determined from the
current data.Comment: 9 pages, 9 figures, accepted for A&
Grouping of orally ingested silica nanomaterials via use of an integrated approach to testing and assessment to streamline risk assessment
Background: Nanomaterials can exist in different nanoforms (NFs). Their grouping may be supported by the formulation of hypotheses which can be interrogated via integrated approaches to testing and assessment (IATA). IATAs are decision trees that guide the user through tiered testing strategies (TTS) to collect the required evidence needed to accept or reject a grouping hypothesis. In the present paper, we investigated the applicability of IATAs for ingested NFs using a case study that includes different silicon dioxide, SiO2 NFs. Two oral grouping hypotheses addressing local and systemic toxicity were identified relevant for the grouping of these NFs and verified through the application of oral IATAs. Following different Tier 1 and/or Tier 2 in vitro methods of the TTS (i.e., in vitro dissolution, barrier integrity and inflammation assays), we generated the NF datasets. Furthermore, similarity algorithms (e.g., Bayesian method and Cluster analysis) were utilized to identify similarities among the NFs and establish a provisional group(s). The grouping based on Tier 1 and/or Tier 2 testing was analyzed in relation to available Tier 3 in vivo data in order to verify if the read-across was possible and therefore support a grouping decision. Results: The measurement of the dissolution rate of the silica NFs in the oro-gastrointestinal tract and in the lysosome identified them as gradually dissolving and biopersistent NFs. For the local toxicity to intestinal epithelium (e.g. cytotoxicity, membrane integrity and inflammation), the biological results of the gastrointestinal tract models indicate that all of the silica NFs were similar with respect to the lack of local toxicity and, therefore, belong to the same group; in vivo data (although limited) confirmed the lack of local toxicity of NFs. For systemic toxicity, Tier 1 data did not identify similarity across the NFs, with results across different decision nodes being inconsistent in providing homogeneous group(s). Moreover, the available Tier 3 in vivo data were also insufficient to support decisions based upon the obtained in vitro results and relating to the toxicity of the tested NFs. Conclusions: The information generated by the tested oral IATAs can be effectively used for similarity assessment to support a grouping decision upon the application of a hypothesis related to toxicity in the gastrointestinal tract. The IATAs facilitated a structured data analysis and, by means of the expert’s interpretation, supported read-across with the available in vivo data. The IATAs also supported the users in decision making, for example, reducing the testing when the grouping was well supported by the evidence and/or moving forward to advanced testing (e.g., the use of more suitable cellular models or chronic exposure) to improve the confidence level of the data and obtain more focused information
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