Cutinases fúngicas: propriedades e aplicações industriais

Cutinases (EC 3.1.1.74) are also known as cutin hidrolases. These enzymes share catalytic properties of lipases and esterases, presenting a unique feature of being active regardless the presence of an oil-water interface, making them interesting as biocatalysts in several industrial processes involving hydrolysis, esterification and trans-esterification reactions. They are also active in different reaction media, allowing their applications in different areas such as food industry, cosmetics, fine chemicals, pesticide and insecticide degradation, treatment and laundry of fiber textiles and polymer chemistry. The present review describes the characteristics, potential applications and new perspectives for these enzymes

Fungal cutinases: properties and industrial applications - review

Cutinases (EC 3.1.1.74) are also known as cutin hidrolases. These enzymes share catalytic properties of lipases and esterases, presenting a unique feature of being active regardless the presence of an oil-water interface, making them interesting as biocatalysts in several industrial processes involving hydrolysis, esterification and trans-esterification reactions. They are also active in different reaction media, allowing their applications in different areas such as food industry, cosmetics, fine chemicals, pesticide and insecticide degradation, treatment and laundry of fiber textiles and polymer chemistry. The present review describes the characteristics, potential applications and new perspectives for these enzymes.21182123Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Seleção de microorganismos produtores de cutinase

Both cutinase and lipase belong to the esterase group of enzymes (EC 3.1.1.X), which are capable of catalyzing the hydrolysis of ester bonds. Cutinase catalyzes the hydrolysis of cutin, an insoluble biopolyester which is the structural component of plant cuticles. As cutinase is an efficient catalyst in watery or organic media, it is potentially appropriate for the detergent, food and cosmetic industries. The objective of this work was to isolate microorganisms from plants and perform a pre-selection of molds showing esterase producing ability. The selected strains were then submitted to fermentation in media containing cutin. The lipolytic and cutinolytic activities of the supernatant were determined in order to differentiate lipase producers from cutinase-producing strains. The mold strain selected as the best cutinase producer was identified as being Fusarium oxysporium.A cutinase pertence, como as lípases, ao grupo das esterases (EC 3.1.1.X), que são enzimas capazes de catalisar reações de hidrólise de ligações do tipo éster. A cutinase catalisa a hidrólise da cutina, um biopoliéster insolúvel que constitui o componente estrutural da cutícula das plantas. Esta enzima tem se mostrado um eficiente catalisador tanto em solução aquosa quanto em meios orgânicos, sendo potencialmente apropriada para usos em indústria de detergentes, alimentos e cosméticos. O objetivo deste trabalho foi isolar fungos de diversas fontes e realizar uma pré-seleção destes, quanto à habilidade em produzir esterase. As linhagens fúngicas pré-selecionadas como produtoras de esterase foram inoculadas em meio de cultivo contendo cutina extraída de maçã. As atividades cutinolítica e lipolítica foram medidas no sobrenadante das culturas a fim de diferenciar os microrganismos produtores de lipase dos produtores de cutinase. Uma linhagem isolada mostrou a maior atividade cutinolítica após 12 dias de fermentação em um meio contendo 1% de cutina e foi identificada como sendo o Fusarium oxysporium.38839

AVALIAÇÃO DE CATCHUP LIGHT BRASILEIROS I: CARACTERÍSTICAS TEMPO-INTENSIDADE E ACEITAÇÃO POR CONSUMIDORES

In the present study samples of ketchup available on the Brazilian market (one traditional and three light versions) were evaluated for their sensory profile and consumer acceptance. The methodology used were the Time-Intensity (T-I) analysis and acceptance tests regarding appearance, flavor, aroma, texture and overall impression. Buying attitude and sweetness perception (‘just-right” test) were also evaluated. The results of  both T-I and acceptance tests showed that, among the light ketchups evaluated in this study, that sweetened with aspartame showed the highest sweetness equivalency and the most similar sensory profile compared to the one sweetened with sucrose. These results indicate that this sweetener, from the evaluated ones, is the most appropriate substitute for sucrose in this application.  This was confirmed by the buying attitude and ‘just-right’ tests.Neste estudo, amostras de catchup disponíveis no mercado brasileiro (uma versão tradicional e três lights) foram avaliadas quanto ao perfil sensorial e à aceitação por consumidores. As metodologias utilizadas foram a análise tempo-intensidade (T-I) e testes de aceitação quanto à aparência, aroma, sabor, textura e impressão global. A atitude de compra em relação aos produtos e a percepção de dulçor (escala do ideal) também foram avaliadas. Os resultados da análise T-I e dos testes de aceitação demonstraram que o catchup light adoçado com aspartame apresentou maior equivalência de dulçor e perfil sensorial mais próximo ao do produto adoçado com sacarose. Concluiu-se que dentre os edulcorantes avaliados, o aspartame é o mais apropriado para essa aplicação. Tal observação foi confirmada  pelos testes de atitude de compra e escala do ideal