2,725 research outputs found

    Microwave Spectroscopy

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    Contains reports on five research projects

    Global Profiling and Inhibition of Protein Lipidation in Vector and Host Stages of the Sleeping Sickness Parasite Trypanosoma brucei

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    The enzyme N-myristoyltransferase (NMT) catalyzes the essential fatty acylation of substrate proteins with myristic acid in eukaryotes and is a validated drug target in the parasite Trypanosoma brucei, the causative agent of African trypanosomiasis (sleeping sickness). N-Myristoylation typically mediates membrane localization of proteins and is essential to the function of many. However, only a handful of proteins are experimentally validated as N-myristoylated in T. brucei. Here, we perform metabolic labeling with an alkyne-tagged myristic acid analogue, enabling the capture of lipidated proteins in insect and host life stages of T. brucei. We further compare this with a longer chain palmitate analogue to explore the chain length-specific incorporation of fatty acids into proteins. Finally, we combine the alkynyl-myristate analogue with NMT inhibitors and quantitative chemical proteomics to globally define N-myristoylated proteins in the clinically relevant bloodstream form parasites. This analysis reveals five ARF family small GTPases, calpain-like proteins, phosphatases, and many uncharacterized proteins as substrates of NMT in the parasite, providing a global view of the scope of this important protein modification and further evidence for the crucial and pleiotropic role of NMT in the cell

    Pressure Induced Hydration Dynamics of Membranes

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    Pressure-jump initiated time-resolved x-ray diffraction studies of dynamics of the hydration of the hexagonal phase in biological membranes show that (i) the relaxation of the unit cell spacing is non-exponential in time; (ii) the Bragg peaks shift smoothly to their final positions without significant broadening or loss in crystalline order. This suggests that the hydration is not diffusion limited but occurs via a rather homogeneous swelling of the whole lattice, described by power law kinetics with an exponent β=1.3±0.2 \beta = 1.3 \pm 0.2.Comment: REVTEX 3, 10 pages,3 figures(available on request),#

    Radio Galaxy Zoo: The Distortion of Radio Galaxies by Galaxy Clusters

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    We study the impact of cluster environment on the morphology of a sample of 4304 extended radio galaxies from Radio Galaxy Zoo. A total of 87% of the sample lies within a projected 15 Mpc of an optically identified cluster. Brightest cluster galaxies (BCGs) are more likely than other cluster members to be radio sources, and are also moderately bent. The surface density as a function of separation from cluster center of non-BCG radio galaxies follows a power law with index 1.10±0.03-1.10\pm 0.03 out to 10 r50010~r_{500} (7 \sim 7~Mpc), which is steeper than the corresponding distribution for optically selected galaxies. Non-BCG radio galaxies are statistically more bent the closer they are to the cluster center. Within the inner 1.5 r5001.5~r_{500} (1 \sim 1~Mpc) of a cluster, non-BCG radio galaxies are statistically more bent in high-mass clusters than in low-mass clusters. Together, we find that non-BCG sources are statistically more bent in environments that exert greater ram pressure. We use the orientation of bent radio galaxies as an indicator of galaxy orbits and find that they are preferentially in radial orbits. Away from clusters, there is a large population of bent radio galaxies, limiting their use as cluster locators; however, they are still located within statistically overdense regions. We investigate the asymmetry in the tail length of sources that have their tails aligned along the radius vector from the cluster center, and find that the length of the inward-pointing tail is weakly suppressed for sources close to the center of the cluster.Comment: 23 pages, 17 figures, 2 tables. Supplemental data files available in The Astronomical Journal or contact autho

    Heating mechanisms in radio frequency driven ultracold plasmas

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    Several mechanisms by which an external electromagnetic field influences the temperature of a plasma are studied analytically and specialized to the system of an ultracold plasma (UCP) driven by a uniform radio frequency (RF) field. Heating through collisional absorption is reviewed and applied to UCPs. Furthermore, it is shown that the RF field modifies the three body recombination process by ionizing electrons from intermediate high-lying Rydberg states and upshifting the continuum threshold, resulting in a suppression of three body recombination. Heating through collisionless absorption associated with the finite plasma size is calculated in detail, revealing a temperature threshold below which collisionless absorption is ineffective.Comment: 14 pages, 7 figure

    Rotating Superconductors and the London Moment: Thermodynamics versus Microscopics

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    Comparing various microscopic theories of rotating superconductors to the conclusions of thermodynamic considerations, we traced their marked difference to the question of how some thermodynamic quantities (the electrostatic and chemical potentials) are related to more microscopic ones: The electron's the work function, mean-field potential and Fermi energy -- certainly a question of general import. After the correct identification is established, the relativistic correction for the London Moment is shown to vanish, with the obvious contribution from the Fermi velocity being compensated by other contributions such as electrostatics and interactions.Comment: 23 pages 4 fi

    Spin foams with timelike surfaces

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    Spin foams of 4d gravity were recently extended from complexes with purely spacelike surfaces to complexes that also contain timelike surfaces. In this article, we express the associated partition function in terms of vertex amplitudes and integrals over coherent states. The coherent states are characterized by unit 3--vectors which represent normals to surfaces and lie either in the 2--sphere or the 2d hyperboloids. In the case of timelike surfaces, a new type of coherent state is used and the associated completeness relation is derived. It is also shown that the quantum simplicity constraints can be deduced by three different methods: by weak imposition of the constraints, by restriction of coherent state bases and by the master constraint.Comment: 22 pages, no figures; v2: remarks on operator formalism added in discussion; correction: the spin 1/2 irrep of the discrete series does not appear in the Plancherel decompositio

    Endomorphisms of abelian varieties, cyclotomic extensions and Lie algebras

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    We prove an analogue of the Tate conjecture on homomorphisms of abelian varieties over infinite cyclotomic extensions of finitely generated fields of characteristic zero.Comment: 9 page

    Microwave Spectroscopy

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    Contains reports on four research projects
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