258 research outputs found
Parallel metatranscriptome analyses of host and symbiont gene expression in the gut of the termite Reticulitermes flavipes
<p>Abstract</p> <p>Background</p> <p>Termite lignocellulose digestion is achieved through a collaboration of host plus prokaryotic and eukaryotic symbionts. In the present work, we took a combined host and symbiont metatranscriptomic approach for investigating the digestive contributions of host and symbiont in the lower termite <it>Reticulitermes flavipes</it>. Our approach consisted of parallel high-throughput sequencing from (i) a host gut cDNA library and (ii) a hindgut symbiont cDNA library. Subsequently, we undertook functional analyses of newly identified phenoloxidases with potential importance as pretreatment enzymes in industrial lignocellulose processing.</p> <p>Results</p> <p>Over 10,000 expressed sequence tags (ESTs) were sequenced from the 2 libraries that aligned into 6,555 putative transcripts, including 171 putative lignocellulase genes. Sequence analyses provided insights in two areas. First, a non-overlapping complement of host and symbiont (prokaryotic plus protist) glycohydrolase gene families known to participate in cellulose, hemicellulose, alpha carbohydrate, and chitin degradation were identified. Of these, cellulases are contributed by host plus symbiont genomes, whereas hemicellulases are contributed exclusively by symbiont genomes. Second, a diverse complement of previously unknown genes that encode proteins with homology to lignase, antioxidant, and detoxification enzymes were identified exclusively from the host library (laccase, catalase, peroxidase, superoxide dismutase, carboxylesterase, cytochrome P450). Subsequently, functional analyses of phenoloxidase activity provided results that were strongly consistent with patterns of laccase gene expression. In particular, phenoloxidase activity and laccase gene expression are mostly restricted to symbiont-free foregut plus salivary gland tissues, and phenoloxidase activity is inducible by lignin feeding.</p> <p>Conclusion</p> <p>To our knowledge, this is the first time that a dual host-symbiont transcriptome sequencing effort has been conducted in a single termite species. This sequence database represents an important new genomic resource for use in further studies of collaborative host-symbiont termite digestion, as well as development of coevolved host and symbiont-derived biocatalysts for use in industrial biomass-to-bioethanol applications. Additionally, this study demonstrates that: (i) phenoloxidase activities are prominent in the <it>R. flavipes </it>gut and are not symbiont derived, (ii) expands the known number of host and symbiont glycosyl hydrolase families in <it>Reticulitermes</it>, and (iii) supports previous models of lignin degradation and host-symbiont collaboration in cellulose/hemicellulose digestion in the termite gut. All sequences in this paper are available publicly with the accession numbers <ext-link ext-link-id="FL634956" ext-link-type="gen">FL634956</ext-link>-<ext-link ext-link-id="FL640828" ext-link-type="gen">FL640828</ext-link> (Termite Gut library) and <ext-link ext-link-id="FL641015" ext-link-type="gen">FL641015</ext-link>-<ext-link ext-link-id="FL645753" ext-link-type="gen">FL645753</ext-link> (Symbiont library).</p
Realizability of metamaterials with prescribed electric permittivity and magnetic permeability tensors
We show that any pair of real symmetric tensors \BGve and \BGm can be
realized as the effective electric permittivity and effective magnetic
permeability of a metamaterial at a given fixed frequency. The construction
starts with two extremely low loss metamaterials, with arbitrarily small
microstructure, whose existence is ensured by the work of Bouchitt{\'e} and
Bourel and Bouchitt\'e and Schweizer, one having at the given frequency a
permittivity tensor with exactly one negative eigenvalue, and a positive
permeability tensor, and the other having a positive permittivity tensor, and a
permeability tensor having exactly one negative eigenvalue. To achieve the
desired effective properties these materials are laminated together in a
hierarchical multiple rank laminate structure, with widely separated length
scales, and varying directions of lamination, but with the largest length scale
still much shorter than the wavelengths and attenuation lengths in the
macroscopic effective medium.Comment: 12 pages, no figure
Weak-strong uniqueness of dissipative measure-valued solutions for polyconvex elastodynamics
For the equations of elastodynamics with polyconvex stored energy, and some
related simpler systems, we define a notion of dissipative measure-valued
solution and show that such a solution agrees with a classical solution with
the same initial data when such a classical solution exists. As an application
of the method we give a short proof of strong convergence in the continuum
limit of a lattice approximation of one dimensional elastodynamics in the
presence of a classical solution. Also, for a system of conservation laws
endowed with a positive and convex entropy, we show that dissipative
measure-valued solutions attain their initial data in a strong sense after time
averaging
Anomalous diffusion for a class of systems with two conserved quantities
We introduce a class of one dimensional deterministic models of energy-volume
conserving interfaces. Numerical simulations show that these dynamics are
genuinely super-diffusive. We then modify the dynamics by adding a conservative
stochastic noise so that it becomes ergodic. System of conservation laws are
derived as hydrodynamic limits of the modified dynamics. Numerical evidence
shows these models are still super-diffusive. This is proven rigorously for
harmonic potentials
Continuous, Semi-discrete, and Fully Discretized Navier-Stokes Equations
The Navier--Stokes equations are commonly used to model and to simulate flow
phenomena. We introduce the basic equations and discuss the standard methods
for the spatial and temporal discretization. We analyse the semi-discrete
equations -- a semi-explicit nonlinear DAE -- in terms of the strangeness index
and quantify the numerical difficulties in the fully discrete schemes, that are
induced by the strangeness of the system. By analyzing the Kronecker index of
the difference-algebraic equations, that represent commonly and successfully
used time stepping schemes for the Navier--Stokes equations, we show that those
time-integration schemes factually remove the strangeness. The theoretical
considerations are backed and illustrated by numerical examples.Comment: 28 pages, 2 figure, code available under DOI: 10.5281/zenodo.998909,
https://doi.org/10.5281/zenodo.99890
A multiscale analysis of drug transport and response for a multiphase tumor model
In this article we consider the spatial homogenization of a multi- phase model for avascular tumour growth and response to chemother- apeutic treatment. The key contribution of this work is the derivation of a system of homogenized partial differential equations describing macroscopic tumour growth, coupled to transport of drug and nutri- ent, that explicitly incorporates details of the structure and dynamics of the tumour at the microscale. In order to derive these equations we employ an asymptotic homogenization of a microscopic description under the assumption of strong interphase drag, periodic microstructure, and strong separation of scales. The resulting macroscale model com- prises a Darcy flow coupled to a system of reaction-advection partial differential equations. The coupled growth, response, and transport dynamics on the tissue scale are investigated via numerical experi- ments for simple academic test cases of microstructural information and tissue geometry, in which we observe drug- and nutrient-regulated growth and response consistent with the anticipated dynamics of the macroscale system
About curvature, conformal metrics and warped products
We consider the curvature of a family of warped products of two
pseduo-Riemannian manifolds and furnished with metrics of
the form and, in particular, of the type , where are smooth
functions and is a real parameter. We obtain suitable expressions for the
Ricci tensor and scalar curvature of such products that allow us to establish
results about the existence of Einstein or constant scalar curvature structures
in these categories. If is Riemannian, the latter question involves
nonlinear elliptic partial differential equations with concave-convex
nonlinearities and singular partial differential equations of the
Lichnerowicz-York type among others.Comment: 32 pages, 3 figure
Small BGK waves and nonlinear Landau damping
Consider 1D Vlasov-poisson system with a fixed ion background and periodic
condition on the space variable. First, we show that for general homogeneous
equilibria, within any small neighborhood in the Sobolev space W^{s,p}
(p>1,s<1+(1/p)) of the steady distribution function, there exist nontrivial
travelling wave solutions (BGK waves) with arbitrary minimal period and
traveling speed. This implies that nonlinear Landau damping is not true in
W^{s,p}(s<1+(1/p)) space for any homogeneous equilibria and any spatial period.
Indeed, in W^{s,p} (s<1+(1/p)) neighborhood of any homogeneous state, the long
time dynamics is very rich, including travelling BGK waves, unstable
homogeneous states and their possible invariant manifolds. Second, it is shown
that for homogeneous equilibria satisfying Penrose's linear stability
condition, there exist no nontrivial travelling BGK waves and unstable
homogeneous states in some W^{s,p} (p>1,s>1+(1/p)) neighborhood. Furthermore,
when p=2,we prove that there exist no nontrivial invariant structures in the
H^{s} (s>(3/2)) neighborhood of stable homogeneous states. These results
suggest the long time dynamics in the W^{s,p} (s>1+(1/p)) and particularly, in
the H^{s} (s>(3/2)) neighborhoods of a stable homogeneous state might be
relatively simple. We also demonstrate that linear damping holds for initial
perturbations in very rough spaces, for linearly stable homogeneous state. This
suggests that the contrasting dynamics in W^{s,p} spaces with the critical
power s=1+(1/p) is a trully nonlinear phenomena which can not be traced back to
the linear level
Character of Christ: A Proposal for Excellence in Christian Character Education
Moral teaching programs, such as character education, have been implemented nationwide in order to curb the growing trend of violence, abuse, and moral relativism within schools, both public and private. These programs represent a variety of moral training philosophies, and current research is revealing some best practices within the field. However, these programs do little to address the needs of distinctively Christian educators who seek to train their students toward the character of Jesus Christ. The research in this study promotes the development of a curriculum to meet this need. The following research indicates that character education\u27s premise and many of its practices are worthy of consideration when developing a Christian character curriculum. However, the foundation of the character traits promoted by a Christian character curriculum must not be based on the consensus of a pluralistic society. The foundation must be established solely on the person of Christ. Best practices within the field of character education are emerging through current research. These practices and the theories behind them are also examined in light of the development of a Christian character curriculum. Recommendations and implications for a Christian character curriculum are made in both theory and practice
Towards a unified theory of Sobolev inequalities
We discuss our work on pointwise inequalities for the gradient which are
connected with the isoperimetric profile associated to a given geometry. We
show how they can be used to unify certain aspects of the theory of Sobolev
inequalities. In particular, we discuss our recent papers on fractional order
inequalities, Coulhon type inequalities, transference and dimensionless
inequalities and our forthcoming work on sharp higher order Sobolev
inequalities that can be obtained by iteration.Comment: 39 pages, made some changes to section 1
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