26 research outputs found
Testicular morphology of adult wistar rats treated with Rudgea viburnoides (Cham.) Benth. leaf infusion
The present study was carried out to investigate the effects of Rudgea viburnoides infusion on the body biometry and testicular morphometry and stereology of adult Wistar rats. Two groups received the infusion daily at the concentration of 3 or 6 mg/mL for 40 days. The control group received only water. Neither the biometrical parameters nor the tubular diameter and the height of the seminiferous epithelium showed any significant alterations in the treated animals. Leydig cells stereology did not show any significant alterations in the treated animals. These results indicated that R. viburnoides did not cause alterations in body biometry and testicular morphometry.10110
Testicular morphology of adult wistar rats treated with Rudgea viburnoides (Cham.) Benth. leaf infusion
Hematology and blood biochemistry in wild hybrid marmosets from the Atlantic Forest, Brazil
Interbular space characterization in adult capybara (Hydrochoerus hydrochaeris) testis
Cinética da espermatogênese de Catetos (Tayassu tajacu)
Objetivou-se, com esta pesquisa caracterizar os estádios do ciclo do epitélio seminífero (CES) pelo método da morfologia tubular, bem como determinar o número de gerações de espermatogônias diferenciadas em catetos adultos. Utilizaram-se oito catetos adultos. Fragmentos de testículo foram incluídos em resina plástica para as análises microscópicas propostas. Foram determinados os diâmetros nucleares das células germinativas, os nucleolares das células de Sertoli e a população de cada um desses tipos celulares. Os estádios do ciclo do epitélio seminíferos foram caracterizados. Concluiu-se que há seis gerações de espermatogônias diferenciadas em catetos (A 1 , A 2 , A 3 , A 4 , In e B) e que a composição celular dos oito estádios do ciclo do epitélio seminífero desses animais é
muito semelhante à de suínos.The aim of this paper was to characterize the stages of the epithelium seminiferous cycle for the tubular morphology method and to determine the differentiated spermatogonia generations number in adult collared peccaries. Eight adult animals were used. Fragments of testis were embedded in plastic resin for microscopic analyses. The germinative cells nuclear diameters and Sertoli cell nucleolar diameters and their respective population were evaluated. Epithelium seminiferous stages were also characterized. In conclusion, this study found six differentiated spermatogonia generations in collared peccaries and that eight epithelium seminiferous cycle stages were very similar to related in swine
Coleta e avaliação do sêmen de catetos (Tayassu tajacu)
Objetivou-se com esta pesquisa testar dois protocolos de eletroejaculação e estudar a motilidade espermática progressiva, vigor, concentração e alterações morfológicas dos espermatozóides de catetos adultos criados em cativeiro. Utilizaram-se seis machos com idade entre 10 e 18 anos. Os animais foram anestesiados com cloridrato de zolazepan e cloridrato de tiletamina e em seguida coletou-se o sêmen com auxílio de um eletroejaculador. O volume médio do ejaculado foi 3,11 + 0,9mL, a motilidade espermática progressiva foi 48,7 ± 31,5%, o vigor, 2,1 + 1,4 e a concentração espermática média, 87,0 + 53,1 x 10(6) sptz/mL. Concluiu-se com esta pesquisa que a utilização de três sessões de 15 estímulos de 12 volts foi suficiente para coleta de sêmen de catetos anestesiados e que os resultados da morfologia espermática da maioria dos catetos trabalhados foram compatíveis com o quadro de degeneração testicular
Capture of a male jaguar using a Minimally Invasive Capture System (MICS) at the Brazilian Atlantic forest
Fieldwork and management of the animals were undertaken under permission nº 54218 of Sistema de Autorização e Informação em Biodiversidade (SISBIO) of Instituto Chico Mendes de Conservação da Biodiversidade (ICMBio) - Ministério do Meio Ambiente (MMA) of Brazil. The capture of large felids was also authorized by the Ethics and Animal Welfare Committee of Universidade Vila Velha (Opinion n° 412-2017 / CEUA-UVV). And the use of live bait was au-thorized by the Ethics and Animal Welfare Committee of Universidade Vila Velha (Opinion n° 435-2017 / CEUA-UVV).The video shows the sequence of the capture of an adult male jaguar at the Vale Natural Reserve (Espíritu Santo, Brazil) on 2nd July 2019. When the jaguar arrived at the capture point he triggered three foot-snares set surrounding the carcass. A few minutes after we remotely (about 300 m from the carcass) shot a dart and immobilized the jaguar. Soon in the afternoon of the day after, the jaguar returned to the site to continue feeding on the carcass already wearing the GPS collar we tagged him.The research was funded by the project CGL2010-16902 of the Spanish Ministry of Research and Innovation, the project CGL2013-46026-P of MINECO, the excellence project RNM 2300 of the Junta de Andalucía, and the project FAPES 510/2016 of the Fundação de Amparo à Pesquisa e Inovação do Espírito Santo.Peer reviewe
First Capture of a Jaguar Using a Minimally Invasive Capture System for GPS Tracking in an Isolated Patch of Atlantic Forest in Southern Brazil
This study presents the first successful capture using GPS tagging of a jaguar (Panthera onca) using a minimally invasive capture system (MICS). We used snare-foot traps and a MICS during two capture campaigns in a fragment of Atlantic Forest in southeastern Brazil. The specimen disarmed snares on different occasions, and capture was only possible with the MICS. The captured jaguar, an estimated 16-year-old adult male, was monitored using a GPS Vertex Plus Iridium collar with an optimal performance of 86% in expected locations. The jaguar’s home range (659 km2 by MPC and 174 km2 by 95%K) was within the observed range for the species and the animal was primarily maintained in protected areas. The habitat types most frequently used were native grassland (27.2% of 4798 fixes), marsh (24.8%), and dense lowland forest (24.7%). The use of a MICS for trapping jaguars is a promising technique that shows advantages in terms of efficiency, selectivity, portability, reduced potential risk of injury to animals or trappers, and animal stress compared to other capture methods used for the species
Identificação e quantificação de gânglios nervosos, células argentafins, argirófilas e imunorreativas à serotonina no ceco da capivara (Hydrochoerus hydrochaeris)
A distribuição regional e a freqüência de gânglios nervosos, células argirófilas, células argentafins e células imunorreativas à serotonina foram estudadas em cinco regiões do ceco de dez capivaras adultas. Para isso, utilizaram-se técnicas específicas de coloração para cada um dos tipos de células endócrinas pesquisadas; e para a quantificação dos gânglios nervosos, submucosos e mioentéricos, empregou-se a técnica da Hematoxilina- IE Omaior número de ganglios submucosos na parede do ceco foi de 3,6/mm2 de área na região de transição ileocecal. O número médio de gânglios mioentéricoslmm2 de área da túnica muscular foi maior nas regiões de transição ileocecal e na tênia, sendo, respectivamente, de 10 e 9,3. 0 número médio de células argirófilas por mini de área de mucosa variou de 235,7 na região de transição ileocecal a I38,ó no ápice; o de células argentafins apresentou uma variação de 224,3 cêlulas/mm2 na base e menor número no ápice, 165,7 células/mmZ de área de mucosa. As células imunoireativas à serotonina foram detectadas em maior número na região de transição ileocecal, com média de 107,1 células/mmZ, e menor no ápice, de 27,1 céiulas/mm2.The rêgional distribution and frequency of nervous ganglia, argírOphyI-, argentaffin- and serotonin immunoreactive cells were studied in five areas of the cecum of ten adult capybaras, Specific stain techniques were used to detect each endocrine cell investigated and the general technique of Haematoxilin-Eosin was used to quantify the Meissner (Submucous) and Auerbach (myenteric) ganglia. The largest mean number of submucosal ganglia observed in the cecum wall was 3.6 ganglíons/mmº in the ileo-cecal transition area. The average number of ganglia myenterics/rnmz in the muscular tunic was larger in the Hee-cecal transition and the taenia areas, 10, and 9.3, respectively. The mean number of argirophyl cells/mmº of the mucous membrane area ranged from 235.7 in the ileo-cecal transition area to 138.6 in the apex area; the number of argentaffin cells showed variation of 224.3 cells/mmº in the base to a smaller number in the apex,‘ 165.7 cells/mmº of the mucous membrane area. The largest number of serotonin-inununoreactive cells was detected in the flee-cecal transition area, the mean number being lO7.l cells/mmº and the smallest number occurring in the apex, representing 27.1 cells/m2