Impact of large wildfires on PM10 levels and human mortality in Portugal

Uncontrolled wildfires have a substantial impact on the environment, the economy and local populations. According to the European Forest Fire Information System (EFFIS), between 2000 and 2013 wildfires burned up to 740 000 ha of land annually in the south of Europe, Portugal being the country with the highest percentage of burned area per square kilometre. However, there is still a lack of knowledge regarding the impacts of the wildfire-related pollutants on the mortality of the country's population. All wildfires occurring during the fire season (June–July–August–September) from 2001 and 2016 were identified, and those with a burned area above 1000 ha (large fires) were considered for the study. During the studied period (2001–2016), more than 2 million ha of forest (929 766 ha from June to September alone) were burned in mainland Portugal. Although large fires only represent less than 1 % of the number of total fires, in terms of burned area their contribution is 46 % (53 % from June to September). To assess the spatial impact of the wildfires, burned areas in each region of Portugal were correlated with PM10 concentrations measured at nearby background air quality monitoring stations. Associations between PM10 and all-cause (excluding injuries, poisoning and external causes) and cause-specific mortality (circulatory and respiratory) were studied for the affected populations using Poisson regression models. A significant positive correlation between burned area and PM10 was found in some regions of Portugal, as well as a significant association between PM10 concentrations and mortality, these being apparently related to large wildfires in some of the regions. The north, centre and inland of Portugal are the most affected areas. The high temperatures and long episodes of drought expected in the future will increase the probabilities of extreme events and therefore the occurrence of wildfires.This work was financially supported by the European Regional Development Fund–Fondo Europeo de Desarrollo Regional (ERDF-FEDER), Spanish Ministry of Economy and Competitiveness/Agencia Estatal de Investigación (grant no. CGL2017-87921-R (ACEX project)) and Project UIDB/00511/2020 of LEPABE (Portuguese national funds through FCT/MCTES PIDDAC). Sofia Augusto was supported by the Portuguese Foundation for Science and Technology (grant no. SFRH/BPD/109382/2015)

Diagnóstico del clima organizacional desde la perspectiva del docente en la Facultad de Ciencias Agrotecnológicas

La investigación tuvo como objetivo evaluar el clima organizacional en la Facultad de Ciencias Agrotecnológicas de la Universidad Autónoma de Chihuahua y a partir de éste se determina el nombre del proyecto: Diagnóstico del clima organizacional desde la perspectiva de los docentes de la Facultad de Ciencias Agrotecnológicas. Este trabajo pretende refrescar un estudio investigativo sobre el clima organizacional que se realizó anteriormente en la Facultad. La combinación de experiencia, práctica, ideas y conocimientos teóricos brindan el soporte necesario para construir y proponer procesos de intervención que establezcan un panorama real y un mejoramiento continuo al clima organizacional encontrado en la Facultad. La investigación es de tipo descriptivo cuantitativo en la modalidad de campo. El total de encuestas realizadas fue de 32

STATegra, a comprehensive multi-omics dataset of B-cell differentiation in mouse

Multi-omics approaches use a diversity of high-throughput technologies to profile the different molecular layers of living cells. Ideally, the integration of this information should result in comprehensive systems models of cellular physiology and regulation. However, most multi-omics projects still include a limited number of molecular assays and there have been very few multi-omic studies that evaluate dynamic processes such as cellular growth, development and adaptation. Hence, we lack formal analysis methods and comprehensive multi-omics datasets that can be leveraged to develop true multi-layered models for dynamic cellular systems. Here we present the STAT egra multi-omics dataset that combines measurements from up to 10 different omics technologies applied to the same biological system, namely the well-studied mouse pre-B-cell differentiation. STATegra include

Identificacion of transthyretin and β4 thymosin as potential biomarkers in aucte coronary syndrome by two independent methods, 2-DE/DIGE and SELDI/TOF

Comunicaciones a congreso

Age-Associated Metabolic and Morphologic Changes in Mitochondria of Individual Mouse and Hamster Oocytes

Background: In human oocytes, as in other mammalian ova, there is a significant variation in the pregnancy potential, with approximately 20% of oocyte-sperm meetings resulting in pregnancies. This frequency of successful fertilization decreases as the oocytes age. This low proportion of fruitful couplings appears to be influenced by changes in mitochondrial structure and function. In this study, we have examined mitochondrial biogenesis in both hamster (Mesocricetus auratus) and mouse (Mus musculus) ova as models for understanding the effects of aging on mitochondrial structure and energy production within the mammalian oocyte. Methodology/Principal Findings: Individual metaphase II oocytes from a total of 25 young and old mice and hamsters were collected from ovarian follicles after hormone stimulation and prepared for biochemical or structural analysis. Adenosine triphosphate levels and mitochondrial DNA number were determined within individual oocytes from young and old animals. In aged hamsters, oocyte adenosine triphosphate levels and mitochondrial DNA molecules were reduced 35.4% and 51.8%, respectively. Reductions of 38.4% and 44% in adenosine triphosphate and mitochondrial genomes, respectively, were also seen in aged mouse oocytes. Transmission electron microscopic (TEM) analysis showed that aged rodent oocytes had significant alterations in mitochondrial and cytoplasmic lamellae structure. Conclusions/Significance: In both mice and hamsters, decreased adenosine triphosphate in aged oocytes is correlated with a similar decrease in mtDNA molecules and number of mitochondria. Mitochondria in mice and hamsters undergo significant morphological change with aging including mitochondrial vacuolization, cristae alterations, and changes in cytoplasmic lamellae

Oxidative stress in pregnancy and fertility pathologies

Oxidative stress designates the state of imbalance between reactive oxygen species (ROS) production and antioxidant levels. In a healthy placenta, there is an increase in ROS production, due to formation of new tissues and inherent metabolism, but this is balanced by higher levels of antioxidants. However, this balance is lost in some situations, with a consequent increase in oxidative stress levels. Oxidative stress has been implicated in several placental disorders and pregnancy pathologies. The present review intends to summarize what is known about the relationship between oxidative stress and well-known pregnancy disorders