Albar-Ramírez, Juan Pablo

Barderas, María G.

Ciordia, Sergio

Cuesta, Fernando de la

Egido, J.

Jiménez-Nacher, J.J.

López-Bescos, L.

Moral, V.

Ríos, Vivian de los

Tarín, N.

Tuñón, J.

Vivanco, Fernando

Repositorio Institucional de la Universidad de Córdoba

Posters S5. Human Proteomics and Biomarkers153P. 84IDENTIFICATION OF TRANSTHYRETIN AND β4-THYMOSIN AS POTENTIAL BIOMARKERS IN ACUTE CORONARY SYNDROME BY TWO INDEPENDENT METHODS, 2-DE/DIGE AND SELDI-TOFCiordia, S1, Moral, V2, De los Ríos, V1, Barderas MG2,3, de la Cuesta F2, Tarín N4, Tuñon J5, Jímenez-Nacher JJ6, López-Bescos L6, Egido J7, *Vivanco, F2 Albar, JP11Unidad de Proteómica, Centro Nacional de Biotecnología/CSIC, Campus de Cantoblanco, E-28049 Madrid. Spain; 2Servicio de Inmunología. Fundación Jiménez Díaz. Madrid; 3Departamento Fisiopatología Vascular, Hospital Nacional de Parapléjicos, SESCAM, Toledo. Spain; 4Departamento de Cardiología, Hospital de Fuenlabrada, Madrid, Spain; 5Departamento de Cardiología, Fundación Jiménez Díaz, Madrid, Spain; 6Departamento de Cardiología, Fundación Hospital de Alcorcón, Madrid, Spain; 7Departamento de Patología Renal, Fundación Jiménez Díaz, Madrid, SpainAcute myocardial infarction (AMI) is one of the leading causes of death in the world and remains a complex pathophysiologic process involving inflammatory, hemostatic and vascular processes. We employed two independent and complementary approaches, SELDI-TOF, and 2-DE/ DIGE in a first phase exploratory biomarker study to analyze modifications in the serum protein map during an acute coronary syndrome (ACS); It disclosed that the levels of two proteins, transthyretin (TTR; 14000 m/z) and acetylated-ß4-thymosin (4970 m/z) were significantly altered in acute coronary syndrome patients in comparison with healthy subjects. TTR was identified by 2-DE/DIGE and SELDI-TOF and confirmed by Western blotting whereas ß4- thymosin was detected only by SELDI-TOF owing to its low molecular mass and confirmed by ELISA and Western blotting. Whereas TTR is involved in the transport of various biologically active compounds ß4- thymosin is essential for cardiomyocyte survival, cardioprotection and repair in the adult heart. Identification of both proteins could help in the understanding of the basis for allowing the diagnosis to be made at an earlier stage of the disease when the treatment is possible.

Identificacion of transthyretin and  β4 thymosin as potential biomarkers in aucte coronary syndrome by two independent methods, 2-DE/DIGE and SELDI/TOF

Identificacion of transthyretin and β4 thymosin as potential biomarkers in aucte coronary syndrome by two independent methods, 2-DE/DIGE and SELDI/TOF

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Repositorio Institucional de la Universidad de Córdoba