Antimicrobial, antioxidant, anti-inflammatory activities and phytoconstituents of extracts from the roots of Dissotis thollonii Cogn. (Melastomataceae)

Abstract Background Dissotis thollonii Cogn. belonging to the Malastomataceae family is used in the West Region of Cameroon for the treatment of inflammation, kidney diseases, pregnancy control and sinusitis. Despite the traditional use of this plant, no scientific report or information was found in the literature regarding neither its biological activity nor its chemical constituents. Aim of the study The present work was designed to determine the antimicrobial, antioxidant and anti-inflammatory activities of different extracts of the roots of D. thollonii Cogn. as well as the isolation and identification of the chemical constituents of this plant. Materials and methods The tests for antimicrobial, antioxidant and anti-inflammatory activities were performed over the MeOH, EtOAc, n-BuOH and aqueous extracts. Compounds were isolated from EtOAc and n-BuOH extracts of the roots of D. thollonii Cogn. through column chromatography and their structures were determined by means of NMR and MS analysis, and published data. Results According to the antimicrobial and antioxidant assays, the EtOAc and n-BuOH extracts were submitted to further separation and purification. This led to the isolation of twelve compounds identified as 3,3′-di- O -methylellagic acid 4′- O-β -D-xylopyranoside 1 , 3- O -methylellagic acid 4′- O-β -D-arabinopyranoside 2 , casuarinin 3 , betulinic acid 4 , β -sitosterol-3- O -D-glucopyranosyl-6′-mirystate 5 , cellobiosylsterol 6 , β -sitosterol 7 , β -sitosterol-3- O-β -D-glucopyranoside 8, arjunolic acid 9 , 3,3′-di- O -methylellagic acid 10 , ellagic acid 11 , and 3,3′-di- O -methylellagic acid 4′- O - β -D-glucopyranoside 12 . The EtOAc extract was the only antimicrobial active sample [diameter of the zone of inhibition (DZI) of 10 mm against Staphyloccocus aureus ] among all the tested extracts. The analysis of fractions of this extract revealed the presence of bioactive compounds with a described antimicrobial activity such as β -sitosterol, β -sitosterol-3- O-β -D-glucopyranoside and arjunolic acid. By using Trolox as the standard drug, all extracts showed antioxidant activity against DPPH in the following order of scavenging ability: Trolox > nBuOH > EtOAc > MeOH > WE (water extract). The ABTS •+ scavenging ability was similar to that found for the DPPH assay, being Trolox > n-BuOH > MeOH > EtOAc > WE. Along with the DPPH and ABTS assays, the FRAP assay showed the scale n-BuOH > MeOH > WE > EtOAc. The phytochemical study of the EtOAc and n-BuOH extracts revealed the presence of important known antioxidant compounds such as ellagic acid derivatives, arjunolic acid, betulinic acid and β -sitosterol. The anti-inflammatory properties of D. thollonii extracts were investigated using RAW 264.7 murine macrophage cells. The MeOH extract reduced the stimulated NO production in a concentration-dependent manner. 86% reduction was observed at the highest tested concentration of 100 μg/ml (IC 50 = 5.9 μg/ml). The n-BuOH extract showed higher dose dependent reduction of NO formation (IC 50 = 6.5 μg/ml) than the EtOAc extract (IC 50 = 18.1 μg/ml), whereas the water extract had no significant influence on the NO production. All the extracts did not have any influence on the macrophage viability. The phytochemical investigation of the EtOAc and n-BuOH extracts revealed that the main compounds identified do have potent anti-inflammatory properties. Conclusion The biological and phytochemical characterization of the root extracts of D. thollonii validates the use of this plant for the treatment of inflammation and sinusitis, thus providing evidence that this plant extracts, as well as some of the isolated compounds, might be potential sources of antioxidant and anti-inflammatory drugs

ANTIMICROBIAL DITERPENOID ALKALOIDS FROM ERYTHROPHLEUM SUAVEOLENS (GUILL. & PERR.) BRENAN

An investigation of the stem bark of Erythrophleum suaveolens (Guill. & Perr.) Brenan yielded the known amide norcassaide (1) and a new diterpenoid alkaloid named norerythrosuaveolide (2) which was characterized as 7β-hydroxy-7-deoxo-6-oxonorcassaide. The structures were established on the basis of one and two-dimensional 1H and 13C NMR spectral data. The compounds showed potent antimicrobial activities against bacteria and yeasts. KEY WORDS: Erythrophleum suaveolens (Guill. & Perr.) Brenan, Norcassaide, Diterpenoid alkaloid, Norerythrosuaveolide, Antimicrobial activities, Bacteria, Yeasts Bull. Chem. Soc. Ethiop. 2005, 19(2), 221-226

<b>Antimicrobial diterpenoid alkaloids from <i>Erythrophleum suaveolens</i> (Guill. & perr.) Brenan</b>

An investigation of the stem bark of Erythrophleum suaveolens (Guill. & Perr.) Brenan yielded the known amide norcassaide (1) and a new diterpenoid alkaloid named norerythrosuaveolide (2) which was characterized as 7&beta;-hydroxy-7-deoxo-6-oxonorcassaide. The structures were established on the basis of one and two-dimensional 1H and 13C NMR spectral data. The compounds showed potent antimicrobial activities against bacteria and yeasts

Toxicidade de óleos essenciais de Piper aduncum e Piper hispidinervum em Sitophilus zeamais Toxicity of essential oils of Piper aduncum and Piper hispidinervum against Sitophilus zeamais

Óleos essenciais de Piper aduncum L. e Piper hispidinervum C.DC. (Piperaceae) foram avaliados quanto ao efeito inseticida em Sitophilus zeamais Motsch. por ação de contato, fumigação e tópica. Na determinação da DL50 e CL50, foi utilizada a análise de Probit. Médias porcentuais de mortalidade dos insetos foram comparadas por meio da análise de regressão linear e superposição das barras do erro padrão. S. zeamais foi mais suscetível ao efeito de contato do óleo de P. hispidinervum em relação ao de P. aduncum, obtendo-se CL50 de 0,51 e 2,87 mL cm- 2 de óleo, respectivamente. Mortalidade próxima a 100% foi obtida nas concentrações de 20 e 30% do óleo de P. hispidinervum. Quanto ao efeito fumigante, a susceptibilidade foi maior no óleo de P. aduncum do que no de P. hispidinervum. Houve diferença significativa entre os óleos somente nas concentrações de 0,1 e 1,0. A DL50 foi semelhante nos dois óleos essenciais por aplicação tópica. No entanto, a mortalidade foi maior com P. aduncum. Óleos essenciais de P. aduncum e P. hispidinervum possuem efeito inseticida em S. zeamais, mas as respostas dependem da concentração e do método de exposição a que o inseto seja submetido.<br>Essential oils of Piper aduncum L. and Piper hispidinervum C. DC. (Piperaceae) were tested against Sitophilus zeamais Motsch. Contact, fumigant toxicity and topical effect were tested. Probit analysis was employed in evaluating the LC50 and LD50 response. Linear regression analysis and superposition of the bars (means error standard) were used for comparison of means percentage mortality. S. zeamais was more susceptible to the contact toxicity of the P. hispidinervum oil than P. aduncum oil with LC50 values of 0.51 and 2.87 mL cm-2 of the oil, respectively. The mortality rate was nearly 100% at P. hispidinervum oil concentrations of 20 and 30%. With respect to the fumigant action, the weevil was more susceptible to the P. aduncum oil than to P. hispidinervum. A significant difference between oils mortality was observed only at 0.1 and 1.0% concentrations. For topical effect, similar values of the LD50 were obtained for essential oils. However, the mortality was higher with P. aduncum. Essential oils of the P. aduncum and P. hispidinervum possess insecticidal effect from S. zeamais, but the responses varied according to concentration of the oils and exposure methods