A practical approach example to measurement uncertainty: Evaluation of 26 immunoassay parameters

IntroductionMeasurement uncertainty is a non-negative parameter that characterizes the distribution of all values appropriate to the measured size and is associated with the measured result. In this study, we aimed to compare the results with various suggestions and produce more qualified results by calculating the measurement uncertainties of the immunoassays like fertility hormones, drug concentration tests, cardiac markers, thyroid function tests and tumour markers. Materials and methodsUncertainty calculation was made in accordance with the top-down approach according to Nordtest guide. The 12-month study of internal and external quality assessment results were used. The parameters of drug concentration tests were performed on the Abbott Architect c8000, other hormones/markers on the i2000 of the same brand. ResultsFactors that increased the measurement uncertainty of a test were due to external quality control data. The calculations showed that 13 of 26 parameters satisfied quality requirements. The highest uncertainty value, with 28% belonged to cancer antigen 19-9 test. The lowest value was calculated for prolactin with 8.3%. Dehydroepiandrosterone sulfate and phenytoin performed poorly in terms of measurement uncertainty, although internal and external quality control assessment results were considered favourable for both. ConclusionIt is recommended that the concept of measurement uncertainty, which plays an important role in the total quality performance of the laboratory, should be followed up by the clinical laboratory experts at certain time intervals and should be increased the awareness of clinicians about the subject

SSR-Based Molecular Identification and Population Structure Analysis for Forage Pea (Pisum sativum var. arvense L.) Landraces

Plant genetic diversity has a significant role in providing traits that can help meet future challenges, such as the need to adapt crops to changing climatic conditions or outbreaks of disease. Our aim in this study was to evaluate the diversity of 61 forage pea specimens (P. sativum ssp. arvense L.) collected from the northeastern Anatolia region of Turkey using 28 simple sequence repeat (SSR) markers. These primers generated a total of 82 polymorphic bands. The number of observed alleles (Na) per primer varied from 2 to 4 with a mean of 2.89 alleles/locus. The mean value of expected heterozygosity (Exp-Het = 0.50) was higher than the mean value of observed heterozygosity (Obs-Het = 0.22). The mean of polymorphic information content (PIC) was 0.41 with a range of 0.03–0.70. The mean number of effective alleles (Ne) was found to be 2.15, Nei’s expected heterozygosity (H) 0.49, and Shannon’s information index (I) 0.81. Cluster analysis through the unweighted pair-group mean average (UPGMA) method revealed that 61 forage pea landraces were divided into three main clusters. Genetic dissimilarity between the genotypes, calculated with the use of NTSYS-pc software, varied between 0.10 (G30 and G34) and 0.66 (G1 and G32). Principal coordinate analysis (PCoA) revealed that three principal coordinates explained 51.54% of the total variation. Moreover, population structure analysis showed that all genotypes formed three sub-populations. Expected heterozygosity values varied between 0.2669 (the first sub-population) and 0.3223 (third sub-population), with an average value of 0.2924. Average population differentiation measurement (Fst) was identified as 0.2351 for the first sub-population, 0.3838 for the second sub-population, and 0.2506 for the third sub-population. In general, current results suggest that SSR markers could be constantly used to illuminate the genetic diversity of forage pea landraces and can potentially be incorporated into future studies that examine the diversity within a larger collection of forage pea genotypes from diverse regions

Incidence of dentinal crack formation during root canal preparation with two NiTi instruments activated by adaptive motion and continuous rotation: An in vitro study

Objectives: The aim of this study was to evaluate dentin crack incidence after root canal instrumentation usingProTaper Next (PT Next; Dentsply Maillefer, Ballaigues, Switzerland) and Twisted File Adaptive (TF Adaptive;Sybron Endo, Orange, California, USA) at different kinematics.Methods: Sixty human extracted premolar teeth were selected and divided into 4 main groups. Group 1: PTNext with continuous rotation (n = 15); Group 2: PT Next with adaptive motion (n = 15); Group 3: TF Adaptivewith continuous rotation (n = 15); Group 4: TF Adaptive with adaptive motion (n = 15). Fifteen unpreparedteeth were used as control group. Crowns of the teeth were removed and roots were sectioned at 3, 6, and 9mm from the apex using a diamond saw. Finally root dentin pieces were evaluated under stereomicroscope atx25 magnification. Digital images were evaluated by 2 researchers.Results: No cracks were observed in the control group. In groups 2 and 4 no fracture was observed at the levelof 9 mm and similarly in group 4 at 6 mm level. Group 3 showed a significantly higher dentin crack formationfollowed by Group 1, 2, 4 and control group respectively (p < 0.039). There was no significant differencebetween groups at 6 mm and 9 mm levels (p = 0.497) except for only 3 mm level (p < 0.035).Conclusions: It was concluded, both adaptive motion and continuous rotationpromoted dentinal defect.Adaptive motion produced less dentinal defects all dentin levels but there was no significant difference

Studies on The Adaptation of Quinoa (Chenopodıum quınoa Willd.) to Eastern Anatolia Region of Turkey

Quinoa (Chenopodium quinoa Willd.), an Andean crop, is native food plant of high nutritional value and its cultivation is increasing rapidly in the World. Background studies should be conducted on the determination of appropriate quinoa varieties for different ecologies for its cultivation to become widespread in a healthy way. This study was conducted in 2015 and 2016 in Erzurum and Igdir non-irrigation conditions in the Eastern Anatolia of Turkey. The locations have different ecological characteristics from each other. The field experiments were conducted with 9 varieties in each location in a randomized complete blocks experimental design with four replications. The grain yield and some related characteristics were examined in the study. The grain yield and related characteristics of quinoa varied significantly depending on varieties and locations in the study. According to the two-year results, quinoa cultivation is a risky in Erzurum which has a high altitude and short vegetation period. The grain yields ranged between 110-599 kg ha-1 and was found to be quite low. In the Igdır location, 933-1646 kg ha-1 of seed yield was obtained from the varieties. The earliest cultivar is Q-52, it has matured in Erzurum at 102 days, Igdir at 118 days. The latest maturation (138 days) was determined in the Oro de Valle cultivar in Igdir conditions. According to these results, moqu Arrochilla, Q-52, Oro de Valle, French Vanilla and Rainbow cultivars can be recommended for non-irrigated conditions of these locations provided that it is seeded early in the spring. However, Erzurum and similar locations are not suitable for the agriculture of this plant due to short plant growing period

Bir Türk alt popülasyonunda kalıcı üst azı dişlerinin kök kanal konfigürasyonunun değerlendirilmesi: Konik ışınlı bilgisayarlı tomografi çalışması

Objective: Maxillary molars may be challenging for root canal treatment due to their complex canal anatomy and additional root canals, especially in the mesiobuccal root. The current study aimed to investigate the prevalence of root and root canal numbers of maxillary molar in a selected Turkish population. Materials and Methods: A total of 905 first and second maxillary molars were evaluated using cone-beam computed tomography (CBCT) images. The number of roots and canals was recorded and the mesiobuccal canal was further evaluated with the Vertucci classification. Results: A total of 394 teeth had a second mesiobuccal (MB2) canal (43.5%). While 90.4% of all maxillary molars had three roots, 44% had four root canals. The most common root canal anatomy of mesiobuccal root canals was Type II (42.6%) followed by Type IV (31.5%) and Type III (22.1%). Conclusions: It is clear that the second mesial root canal in permanent maxillary molars should be carefully searched for the long-term success of root canal treatments. It is seen that CBCT sections will be beneficial in diagnosis and treatment in better understanding the anatomical structure of the teeth and determining possible anatomical deviations.Amaç: Üst çene azı dişleri, karmaşık kök kanal anatomisi ve özellikle mezial kökteki ek kök kanal sayıları nedeniyle endodontik tedaviyi zorlaştırabilir. Bu nedenle başarılı bir kanal tedavisi için üst çene azı dişlerinin anatomik özelliklerinin analiz edilmesi gerekmektedir. Bu çalışmanın amacı, seçilmiş bir Türk nüfusunda üst çene azı dişlerinin kök ve kök kanal sayılarının prevalansını araştırmaktır. Materyal ve Metot: Konik ışınlı bilgisayarlı tomografi (KIBT) kullanılarak toplam 905 birinci ve ikinci kalıcı üst azı dişi değerlendirildi. Kök ve kök kanal sayıları kaydedilerek mezyobukkal meziobukkal kök kanalı Vertucci sınıflamasına göre değerlendirildi. Bulgular: Toplam 394 üst azıda ikinci bir mezial (MB2) kök kanalı (% 43,5) vardı. Tüm üst büyük azı dişlerinin %90,4' ünde üç adet kök varken, %44'ünde ise 4 kök kanalı vardı. Mezial kök kanallarının en yaygın kök kanal anatomisi Tip II (% 42,6) iken, bunu Tip IV (% 31,5) ve Tip III (% 22,1) takip etti. Sonuç: Üst daimi azı dişlerindeki ikinci mezial kök kanalının, kanal tedavilerinin uzun dönem başarısı için dikkatlice aranmasında büyük fayda olduğu açıktır. Dişlerinin anatomik yapısının daha iyi anlaşılmasında ve olası anatomik sapmaların belirlenmesinde KIBT kesitlerinin teşhis ve tedaviye oldukça yardımcı olacağı görülmektedir

First Collection and Identification of Wild Mushrooms in Western Iraq

Fungi are found everywhere and affect our lives every day from mushroom to industrially important product, plant helpers, plant pathogens and to human diseases. Therefore, we concentrated our attention on fungi that can be grown in our environment, while maintaining an interest in their performance in nature. About fifteen different species were collected and identified from different villages of Heet district, Anbar province, Iraq. Out of the fifteen, two species, naming Armillaria mellea and Coprinus disseminates were identified as species level and others: Pleurotus spp., Agaricus spp., Calvatia sp., Telephora sp., Fomes sp., Lepiota sp. and Morchella sp. as genus level only. This study was aimed to collect and identify wild mushrooms that grow naturally in different orchards and gardens on 2009-2013 in Heet city. A total of 15 species in 9 genera, 9 families and 5 orders belong to woody and fleshy species were identified for the first time in Anbar province. The collected mushrooms were appearing from November to February of each year

Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture

The possibility of using in situ addition of anion-exchange resin for the removal of acetate in the culture aimed at improving growth of E. coli and expression of periplasmic human interferon-α2b (PrIFN-α2b) was studied in shake flask culture and stirred tank bioreactor. Different types of anion-exchange resin were evaluated and the concentration of anion-exchange resin was optimized using response surface methodology. The addition of anion-exchange resins reduced acetate accumulation in the culture, which in turn, improved growth of E. coli and enhanced PrIFN-α2b expression. The presence of anion-exchange resins did not influence the physiology of the cells. The weak base anion-exchange resins, which have higher affinity towards acetate, yielded higher PrIFN-α2b expression as compared to strong anion-exchange resins. High concentrations of anion-exchange resin showed inhibitory effect towards growth of E. coli as well as the expression of PrIFN-α2b. The maximum yield of PrIFN-α2b in shake flask culture (501.8 μg/L) and stirred tank bioreactor (578.8 μg/L) was obtained at ion exchange resin (WA 30) concentration of 12.2 g/L. The production of PrIFN-α2b in stirred tank bioreactor with the addition of ion exchange resin was about 1.8-fold higher than that obtained in fermentation without ion exchange resin (318.4 μg/L)