Orange maize in Zambia: crop development and delivery experience

Biofortified vitamin A “orange” maize can help address the adverse health effects of vitamin A deficiency. By 2016, HarvestPlus and its partners had developed six orange maize varieties and delivery efforts have reached more than 100,000 farming households in Zambia. HarvestPlus has established the proof of concept, that vitamin A maize varieties can be developed without compromising yield levels and that these varieties can deliver sufficient quantities of vitamin A to improve nutrition. The delivery program has also shown that farmers are willing to grow orange maize varieties and consumers are willing to buy and eat orange maize products. This paper summarizes the country’s nutritional and consumer backgrounds, the crop development and release of orange maize varieties, the delivery efforts in Zambia and impact measurement. It also synthesizes lessons learned and future challenges.Keywords: Biofortification, Vitamin A Deficiency, Orange Maize, Vitamin A Maize, Zambi

Micronutrient (provitamin A and iron/zinc) retention in biofortified crops

For biofortification to be successful, biofortified crops must demonstrate sufficient levels of retention of micronutrients after typical processing, storage, and cooking practices. Expected levels of retention at the breeding stage were verified experimentally. It was proven that the variety of biofortified crop, processing method, and micronutrient influence the level of retention. Provitamin A is best retained when the crops are boiled/steamed in water. Processing methods that are harsher on the food matrix (i.e. drying, frying, roasting) result in higher losses of provitamin A carotenoids. Degradation also occurs during the storage of dried products (e.g. from sweet potato, maize, cassava) at ambient temperature, and a short shelf life is a constraint that should be considered when biofortified foods. Iron and zinc retention were high for common beans (Phaseolus vulgaris) and cowpeas (Vigna unguiculata), indicating that iron and zinc were mostly preserved during cooking (with/without soaking in water)

Growth temperature and genotype both play important roles in sorghum grain phenolic composition.

Polyphenols in sorghum grains are a source of dietary antioxidants. Polyphenols in six diverse sorghum genotypes grown under two day/night temperature regimes of optimal temperature (OT, 32/21 °C and high temperature (HT, 38/21 °C) were investigated. A total of 23 phenolic compounds were positively or tentatively identified by HPLC-DAD-ESIMS. Compared with other pigmented types, the phenolic profile of white sorghum PI563516 was simpler, since fewer polyphenols were detected. Brown sorghum IS 8525 had the highest levels of caffeic and ferulic acid, but apigenin and luteolin were not detected. Free luteolinidin and apigeninidin levels were lower under HT than OT across all genotypes (p ≤ 0.05), suggesting HT could have inhibited 3-deoxyanthocyanidins formation. These results provide new information on the effects of HT on specific polyphenols in various Australian sorghum genotypes, which might be used as a guide to grow high antioxidant sorghum grains under projected high temperature in the future

Carotenoids retention in biofortified yellow cassava processed with traditional African methods.

Biofortified yellow cassava is being cultivated in countries with high cassava consumption to improve its population's vitamin A status. The carotenoid retention in biofortified cassava when processed as boiled, fufu, and chikwangue was evaluated in this study. Commercial biofortified varieties Kindisa and Vuvu and the experimental genotypes MVZ2011B/360 and MVZ2012/044 were used. Fresh cassava roots were processed as boiled, fufu, and chikwangue. Provitamin A carotenoids (pVACs) content of fresh and processed cassava was measured by high‐performance liquid chromatography, and total carotenoids was measured by spectrophotometer

Subnational Prioritization for Biofortification Interventions in Nigeria

Globally, two billion people suffer from micronutrient malnutrition. Biofortification, the process of breeding staple food crops to have higher micronutrient content, has proven to be efficacious and cost-effective in addressing micronutrient malnutrition. To determine where and in which crop-micronutrient combinations to invest, a global Biofortification Prioritization Index (BPI) was developed (Asare-Marfo et al., 2013). While a country s rank in the global context is useful, it is not granular enough to develop strategies within heterogenous countries. Therefore, this paper utilizes methodology to develop a subnational-level BPI for Nigeria, a country which shows promise for biofortified crops. The subnational BPI is based on three sub-indices: production, consumption, and micronutrient deficiency. In addition, targeted areas are classified as areas of: (1) impact and intervention, (2) impact, or (3) intervention. Sensitivity analyses tested the robustness of BPI results on single sub-index parameters. For vitamin A maize s introduction, the North East and North West zones offer the most promise while the southern zones generate the greatest impact for the introduction of vitamin A cassava. Concentrating vitamin A sweet potato investments in the North Central zone is the most effective while focusing in the North West is the most promising strategy for iron pearl millet. Acknowledgement : The authors would sincerely like to thank Dr. Erick Boy, Head of the HarvestPlus Nutrition Research Unit, and Ms. Amarachi Utah for their consultation and support of this research